FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893487872> ?p ?o ?g. }

• W2893487872 abstract "The Cry11 family belongs to a large group of -endotoxins that share three distinct structural domains. Among the dipteran-active toxins referred to as three-domain Cry11 toxins, the Cry11Aa protein from Bacillus thuringiensis subsp. israelensis (Bti) has been the most extensively studied. Despite the potential of Bti as an effective biological control agent, the understanding of Cry11 toxins remains incomplete. In this study, five Cry11 variants obtained via DNA shuffling displayed toxic activity against Aedes aegypti and Culex quinquefasciatus. Three of these Cry11 variants (8, 23 and 79) were characterized via 3D modeling and analysis of docking with ALP1. The relevant mutations in these variants, such as deletions, insertions and point mutations, are discussed in relation to their structural domains, toxic activities and toxin-receptor interactions. Importantly, deletion of the N-terminal segment in domain I was not associated with any change in toxic activity, and domain III exhibited higher sequence variability than domains I and II. Variant 8 exhibited up to 3.78- and 6.09-fold higher toxicity to A. aegypti than Cry11Bb and Cry11Aa, respectively. Importantly, variant 79 showed an -helix conformation at the C-terminus and formed crystals retaining toxic activity. These findings indicate that five Cry11 variants were preferentially reassembled from the cry11Aa gene during DNA shuffling. The mutations described in loop 2 and loop 3 of domain II provide valuable information regarding the activity of Cry11 toxins against A. aegypti and C. quinquefasciatus larvae and reveal new insights into the application of directed evolution strategies to study the genetic variability of specific domains in cry11 family genes." @default.
• W2893487872 created "2018-10-05" @default.
• W2893487872 creator A5007168438 @default.
• W2893487872 creator A5012654851 @default.
• W2893487872 creator A5030395757 @default.
• W2893487872 creator A5036314011 @default.
• W2893487872 creator A5041873604 @default.
• W2893487872 creator A5049347038 @default.
• W2893487872 creator A5053070610 @default.
• W2893487872 creator A5068465028 @default.
• W2893487872 date "2018-10-17" @default.
• W2893487872 modified "2023-10-15" @default.
• W2893487872 title "Toxic Activity, Molecular Modeling and Docking Simulations of Bacillus thuringiensis Cry11 Toxin Variants Obtained via DNA Shuffling" @default.
• W2893487872 cites W1561598078 @default.
• W2893487872 cites W1982445623 @default.
• W2893487872 cites W1989373372 @default.
• W2893487872 cites W1989443493 @default.
• W2893487872 cites W1990700714 @default.
• W2893487872 cites W1996708925 @default.
• W2893487872 cites W1998415584 @default.
• W2893487872 cites W2002910225 @default.
• W2893487872 cites W2004913131 @default.
• W2893487872 cites W2013202953 @default.
• W2893487872 cites W2014005919 @default.
• W2893487872 cites W2022207223 @default.
• W2893487872 cites W2023015092 @default.
• W2893487872 cites W2023093573 @default.
• W2893487872 cites W2023887952 @default.
• W2893487872 cites W2041951834 @default.
• W2893487872 cites W2044321722 @default.
• W2893487872 cites W2046889205 @default.
• W2893487872 cites W2063227014 @default.
• W2893487872 cites W2064494048 @default.
• W2893487872 cites W2065100367 @default.
• W2893487872 cites W2065585101 @default.
• W2893487872 cites W2065622780 @default.
• W2893487872 cites W2066600296 @default.
• W2893487872 cites W2071086763 @default.
• W2893487872 cites W2071223520 @default.
• W2893487872 cites W2078675196 @default.
• W2893487872 cites W2081335753 @default.
• W2893487872 cites W2088463274 @default.
• W2893487872 cites W2091144460 @default.
• W2893487872 cites W2094245216 @default.
• W2893487872 cites W2096847727 @default.
• W2893487872 cites W2097459266 @default.
• W2893487872 cites W2102361259 @default.
• W2893487872 cites W2106882534 @default.
• W2893487872 cites W2106928585 @default.
• W2893487872 cites W2125045813 @default.
• W2893487872 cites W2128434050 @default.
• W2893487872 cites W2128635872 @default.
• W2893487872 cites W2168055071 @default.
• W2893487872 cites W2295378318 @default.
• W2893487872 cites W2299890380 @default.
• W2893487872 cites W2302327476 @default.
• W2893487872 cites W2340576491 @default.
• W2893487872 cites W2345218814 @default.
• W2893487872 cites W2509902212 @default.
• W2893487872 cites W2528192716 @default.
• W2893487872 cites W2621282535 @default.
• W2893487872 cites W2771829128 @default.
• W2893487872 cites W4293247451 @default.
• W2893487872 doi "https://doi.org/10.3389/fmicb.2018.02461" @default.
• W2893487872 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6199390" @default.
• W2893487872 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30386315" @default.
• W2893487872 hasPublicationYear "2018" @default.
• W2893487872 type Work @default.
• W2893487872 sameAs 2893487872 @default.
• W2893487872 citedByCount "15" @default.
• W2893487872 countsByYear W28934878722020 @default.
• W2893487872 countsByYear W28934878722021 @default.
• W2893487872 countsByYear W28934878722022 @default.
• W2893487872 countsByYear W28934878722023 @default.
• W2893487872 crossrefType "journal-article" @default.
• W2893487872 hasAuthorship W2893487872A5007168438 @default.
• W2893487872 hasAuthorship W2893487872A5012654851 @default.
• W2893487872 hasAuthorship W2893487872A5030395757 @default.
• W2893487872 hasAuthorship W2893487872A5036314011 @default.
• W2893487872 hasAuthorship W2893487872A5041873604 @default.
• W2893487872 hasAuthorship W2893487872A5049347038 @default.
• W2893487872 hasAuthorship W2893487872A5053070610 @default.
• W2893487872 hasAuthorship W2893487872A5068465028 @default.
• W2893487872 hasBestOaLocation W28934878721 @default.
• W2893487872 hasConcept C104317684 @default.
• W2893487872 hasConcept C143065580 @default.
• W2893487872 hasConcept C159110408 @default.
• W2893487872 hasConcept C169627665 @default.
• W2893487872 hasConcept C173758957 @default.
• W2893487872 hasConcept C176944494 @default.
• W2893487872 hasConcept C181199279 @default.
• W2893487872 hasConcept C2776217558 @default.
• W2893487872 hasConcept C2777367657 @default.
• W2893487872 hasConcept C2777775583 @default.
• W2893487872 hasConcept C2779035793 @default.
• W2893487872 hasConcept C2779454882 @default.
• W2893487872 hasConcept C41685203 @default.
• W2893487872 hasConcept C501734568 @default.
• W2893487872 hasConcept C523546767 @default.
• W2893487872 hasConcept C54355233 @default.

• next