Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893497242> ?p ?o ?g. }
- W2893497242 abstract "Oxidative stress plays an important role in the pathology of liver disorders. Total C‑21 steroidal glycosides (TCSGs), isolated from the root tuber of Cynanchum auriculatum Royle ex Wight, have been reported to exert numerous effects, including liver protective and antioxidant effects. In order to investigate the potential mechanisms underlying the protective effects of TCSGs on liver function, the present study used the human normal liver cell line, L02, to evaluate the effects of TCSGs on hydrogen peroxide (H2O2)‑induced oxidative injury and inflammatory responses. The L02 cells were pretreated with various concentrations of TCSGs, followed by exposure to 1.5 mM H2O2. Cell viability was determined by a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and nitric oxide (NO) were measured using colorimetric assays. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH‑Px) and the production of malondialdehyde (MDA) were also determined. Intracellular reactive oxygen species (ROS) levels were detected using a fluorescent probe. H2O2‑induced oxidative toxicity was attenuated following treatment with TCSGs, as indicated by the increase in cell viability, the decreased levels of ALT, AST, LDH, NO, MDA and ROS, and the increased activities of SOD, CAT and GSH‑Px. To further explore the possible mechanisms of action of TCSGs, the nuclear factor erythroid 2‑related factor 2 (Nrf2) and nuclear factor‑κB (NF)‑κB pathways were examined. The results revealed that treatment with TCSGs markedly induced Nrf2 nuclear translocation and upregulated the expression of heme oxygenase‑1 (HO‑1) in the L02 cells damaged by H2O2. In addition, pretreatment with TCSGs inhibited the NF‑κB signaling pathway by blocking the degradation of the inhibitor of nuclear factor κBα (IκBα), thereby reducing the expression and nuclear translocation of NF‑κB, as well as reducing the expression of tumor necrosis factor‑α (TNF‑α), interleukin-6 (IL‑6), inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX‑2). On the whole, the findings of this study demonstrate that TCSGs can protect L02 cells against H2O2‑induced oxidative toxicity and inflammatory injury by increasing the expression of Nrf2 and HO‑1, mediated by the NF‑κB signaling pathway." @default.
- W2893497242 created "2018-10-05" @default.
- W2893497242 creator A5008637608 @default.
- W2893497242 creator A5013539466 @default.
- W2893497242 creator A5014902770 @default.
- W2893497242 creator A5026853598 @default.
- W2893497242 creator A5052226015 @default.
- W2893497242 creator A5052281339 @default.
- W2893497242 creator A5052291030 @default.
- W2893497242 creator A5061980749 @default.
- W2893497242 creator A5079497974 @default.
- W2893497242 creator A5080885895 @default.
- W2893497242 creator A5090273839 @default.
- W2893497242 date "2018-09-25" @default.
- W2893497242 modified "2023-09-25" @default.
- W2893497242 title "Total C-21 steroidal glycosides, isolated from the root tuber of Cynanchum�auriculatum Royle ex Wight, attenuate hydrogen peroxide-induced oxidative injury and inflammation in L02 cells" @default.
- W2893497242 cites W1247569718 @default.
- W2893497242 cites W1518992915 @default.
- W2893497242 cites W1537971445 @default.
- W2893497242 cites W1968755349 @default.
- W2893497242 cites W1979990467 @default.
- W2893497242 cites W1989309487 @default.
- W2893497242 cites W1992314135 @default.
- W2893497242 cites W1993617402 @default.
- W2893497242 cites W1996219280 @default.
- W2893497242 cites W1999820331 @default.
- W2893497242 cites W2004256319 @default.
- W2893497242 cites W2007867750 @default.
- W2893497242 cites W2008329490 @default.
- W2893497242 cites W2013103361 @default.
- W2893497242 cites W2014109189 @default.
- W2893497242 cites W2020053268 @default.
- W2893497242 cites W2023427279 @default.
- W2893497242 cites W2033347574 @default.
- W2893497242 cites W2033847143 @default.
- W2893497242 cites W2049048694 @default.
- W2893497242 cites W2056848799 @default.
- W2893497242 cites W2067917830 @default.
- W2893497242 cites W2070647828 @default.
- W2893497242 cites W2077884098 @default.
- W2893497242 cites W2086810590 @default.
- W2893497242 cites W2090126018 @default.
- W2893497242 cites W2092204583 @default.
- W2893497242 cites W2095002839 @default.
- W2893497242 cites W2096000363 @default.
- W2893497242 cites W2100012786 @default.
- W2893497242 cites W2107277218 @default.
- W2893497242 cites W2117800935 @default.
- W2893497242 cites W2126146739 @default.
- W2893497242 cites W2132925127 @default.
- W2893497242 cites W2139956257 @default.
- W2893497242 cites W2142089445 @default.
- W2893497242 cites W2144638234 @default.
- W2893497242 cites W2146558971 @default.
- W2893497242 cites W2146719839 @default.
- W2893497242 cites W2213439097 @default.
- W2893497242 cites W2259960193 @default.
- W2893497242 cites W227176005 @default.
- W2893497242 cites W2315146917 @default.
- W2893497242 cites W2332599102 @default.
- W2893497242 cites W2354687276 @default.
- W2893497242 cites W2374272717 @default.
- W2893497242 cites W2409480575 @default.
- W2893497242 cites W2431012553 @default.
- W2893497242 cites W2467527170 @default.
- W2893497242 cites W2511997169 @default.
- W2893497242 cites W2514499016 @default.
- W2893497242 cites W2533320009 @default.
- W2893497242 cites W2546744325 @default.
- W2893497242 cites W2568510753 @default.
- W2893497242 cites W2583402497 @default.
- W2893497242 cites W2588442940 @default.
- W2893497242 cites W2590053833 @default.
- W2893497242 cites W2626648993 @default.
- W2893497242 cites W2626912469 @default.
- W2893497242 cites W2740635793 @default.
- W2893497242 cites W2795118526 @default.
- W2893497242 cites W4248078302 @default.
- W2893497242 cites W1964184380 @default.
- W2893497242 doi "https://doi.org/10.3892/ijmm.2018.3896" @default.
- W2893497242 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6202073" @default.
- W2893497242 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30272289" @default.
- W2893497242 hasPublicationYear "2018" @default.
- W2893497242 type Work @default.
- W2893497242 sameAs 2893497242 @default.
- W2893497242 citedByCount "6" @default.
- W2893497242 countsByYear W28934972422019 @default.
- W2893497242 countsByYear W28934972422020 @default.
- W2893497242 countsByYear W28934972422021 @default.
- W2893497242 countsByYear W28934972422022 @default.
- W2893497242 countsByYear W28934972422023 @default.
- W2893497242 crossrefType "journal-article" @default.
- W2893497242 hasAuthorship W2893497242A5008637608 @default.
- W2893497242 hasAuthorship W2893497242A5013539466 @default.
- W2893497242 hasAuthorship W2893497242A5014902770 @default.
- W2893497242 hasAuthorship W2893497242A5026853598 @default.
- W2893497242 hasAuthorship W2893497242A5052226015 @default.
- W2893497242 hasAuthorship W2893497242A5052281339 @default.
- W2893497242 hasAuthorship W2893497242A5052291030 @default.
- W2893497242 hasAuthorship W2893497242A5061980749 @default.