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• W2893505954 abstract "We used DNA that was covalently labeled with fluorescent nucleotides to investigate the transformation process of Bacillus subtilis at the molecular level. We show that the labeled DNA colocalizes with components of the competence machinery, the chromosome, and the recombination protein RecA. Using time-lapse microscopy and microfluidics, we visualized, in real-time, the uptake of fluorescently labeled DNA. We found that under these conditions, cell division is not required for the expression of integrated DNA. Because the competence machinery is conserved in naturally competent bacteria, this method can also be used to investigate the transformation process in many other bacterial species." @default.
• W2893505954 created "2018-10-05" @default.
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• W2893505954 date "2018-11-07" @default.
• W2893505954 modified "2023-09-30" @default.
• W2893505954 title "Fluorescently Labeled DNA Interacts with Competence and Recombination Proteins and Is Integrated and Expressed Following Natural Transformation of Bacillus subtilis" @default.
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• W2893505954 doi "https://doi.org/10.1128/mbio.01161-18" @default.
• W2893505954 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6156202" @default.
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• W2893505954 hasPublicationYear "2018" @default.
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