FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893530958> ?p ?o ?g. }

• W2893530958 abstract "In colorectal cancer, CDX2 expression is lost in approximately 20% of cases and associated with poor outcome. Here, we aim to validate the clinical impact of CDX2 and investigate the role of promoter methylation and histone deacetylation in CDX2 repression and restoration.CDX2 immunohistochemistry was performed on multi-punch tissue microarrays (n = 637 patients). Promoter methylation and protein expression investigated on 11 colorectal cancer cell lines identified two CDX2 low expressors (SW620, COLO205) for treatment with decitabine (DNA methyltransferase inhibitor), trichostatin A (TSA) (general HDAC inhibitor), and LMK-235 (specific HDAC4 and HDAC5 inhibitor). RNA and protein levels were assessed. HDAC5 recruitment to the CDX2 gene promoter region was tested by chromatin immunoprecipitation.Sixty percent of tumors showed focal CDX2 loss; 5% were negative. Reduced CDX2 was associated with lymph node metastasis (p = 0.0167), distant metastasis (p = 0.0123), and unfavorable survival (multivariate analysis: p = 0.0008; HR (95%CI) 0.922 (0.988-0.997)) as well as BRAFV600E, mismatch repair deficiency, and CpG island methylator phenotype. Decitabine treatment alone induced CDX2 RNA and protein with values from 2- to 25-fold. TSA treatment ± decitabine also led to successful restoration of RNA and/or protein. Treatment with LMK-235 alone had marked effects on RNA and protein levels, mainly in COLO205 cells that responded less to decitabine. Lastly, decitabine co-treatment was more effective than LMK-235 alone at restoring CDX2.CDX2 loss is an adverse prognostic factor and linked to molecular features of the serrated pathway. RNA/protein expression is restored in CDX2 low-expressing CRC cell lines by demethylation and HDAC inhibition. Importantly, our data underline HDAC4 and HDAC5 as new epigenetic CDX2 regulators that warrant further investigation." @default.
• W2893530958 created "2018-10-05" @default.
• W2893530958 creator A5009057979 @default.
• W2893530958 creator A5009846567 @default.
• W2893530958 creator A5015962203 @default.
• W2893530958 creator A5020867690 @default.
• W2893530958 creator A5024672280 @default.
• W2893530958 creator A5026430348 @default.
• W2893530958 creator A5029942157 @default.
• W2893530958 creator A5032823288 @default.
• W2893530958 creator A5042466665 @default.
• W2893530958 creator A5060747879 @default.
• W2893530958 creator A5067301442 @default.
• W2893530958 creator A5069558924 @default.
• W2893530958 creator A5072927786 @default.
• W2893530958 creator A5075066220 @default.
• W2893530958 creator A5076003067 @default.
• W2893530958 creator A5077572047 @default.
• W2893530958 creator A5078295184 @default.
• W2893530958 creator A5088384165 @default.
• W2893530958 creator A5091797746 @default.
• W2893530958 date "2018-09-26" @default.
• W2893530958 modified "2023-10-17" @default.
• W2893530958 title "CDX2 in colorectal cancer is an independent prognostic factor and regulated by promoter methylation and histone deacetylation in tumors of the serrated pathway" @default.
• W2893530958 cites W1778923464 @default.
• W2893530958 cites W1889149373 @default.
• W2893530958 cites W1934094702 @default.
• W2893530958 cites W1964979387 @default.
• W2893530958 cites W1967371432 @default.
• W2893530958 cites W1982533604 @default.
• W2893530958 cites W1989264010 @default.
• W2893530958 cites W1990204500 @default.
• W2893530958 cites W1998340711 @default.
• W2893530958 cites W2006734059 @default.
• W2893530958 cites W2015174770 @default.
• W2893530958 cites W2022792299 @default.
• W2893530958 cites W2034873700 @default.
• W2893530958 cites W2035126508 @default.
• W2893530958 cites W2038188490 @default.
• W2893530958 cites W2041434532 @default.
• W2893530958 cites W2060591619 @default.
• W2893530958 cites W2093524469 @default.
• W2893530958 cites W2097018007 @default.
• W2893530958 cites W2102346197 @default.
• W2893530958 cites W2137195081 @default.
• W2893530958 cites W2159118517 @default.
• W2893530958 cites W2164353972 @default.
• W2893530958 cites W2301536592 @default.
• W2893530958 cites W2519941373 @default.
• W2893530958 cites W2535009445 @default.
• W2893530958 cites W2570447124 @default.
• W2893530958 cites W2586745507 @default.
• W2893530958 cites W2606203776 @default.
• W2893530958 cites W2952481429 @default.
• W2893530958 cites W3149222187 @default.
• W2893530958 cites W4231545474 @default.
• W2893530958 doi "https://doi.org/10.1186/s13148-018-0548-2" @default.
• W2893530958 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6158822" @default.
• W2893530958 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30257705" @default.
• W2893530958 hasPublicationYear "2018" @default.
• W2893530958 type Work @default.
• W2893530958 sameAs 2893530958 @default.
• W2893530958 citedByCount "36" @default.
• W2893530958 countsByYear W28935309582019 @default.
• W2893530958 countsByYear W28935309582020 @default.
• W2893530958 countsByYear W28935309582021 @default.
• W2893530958 countsByYear W28935309582022 @default.
• W2893530958 countsByYear W28935309582023 @default.
• W2893530958 crossrefType "journal-article" @default.
• W2893530958 hasAuthorship W2893530958A5009057979 @default.
• W2893530958 hasAuthorship W2893530958A5009846567 @default.
• W2893530958 hasAuthorship W2893530958A5015962203 @default.
• W2893530958 hasAuthorship W2893530958A5020867690 @default.
• W2893530958 hasAuthorship W2893530958A5024672280 @default.
• W2893530958 hasAuthorship W2893530958A5026430348 @default.
• W2893530958 hasAuthorship W2893530958A5029942157 @default.
• W2893530958 hasAuthorship W2893530958A5032823288 @default.
• W2893530958 hasAuthorship W2893530958A5042466665 @default.
• W2893530958 hasAuthorship W2893530958A5060747879 @default.
• W2893530958 hasAuthorship W2893530958A5067301442 @default.
• W2893530958 hasAuthorship W2893530958A5069558924 @default.
• W2893530958 hasAuthorship W2893530958A5072927786 @default.
• W2893530958 hasAuthorship W2893530958A5075066220 @default.
• W2893530958 hasAuthorship W2893530958A5076003067 @default.
• W2893530958 hasAuthorship W2893530958A5077572047 @default.
• W2893530958 hasAuthorship W2893530958A5078295184 @default.
• W2893530958 hasAuthorship W2893530958A5088384165 @default.
• W2893530958 hasAuthorship W2893530958A5091797746 @default.
• W2893530958 hasBestOaLocation W28935309581 @default.
• W2893530958 hasConcept C101762097 @default.
• W2893530958 hasConcept C104317684 @default.
• W2893530958 hasConcept C121587040 @default.
• W2893530958 hasConcept C131364181 @default.
• W2893530958 hasConcept C134320426 @default.
• W2893530958 hasConcept C150194340 @default.
• W2893530958 hasConcept C153911025 @default.
• W2893530958 hasConcept C190727270 @default.
• W2893530958 hasConcept C2777865847 @default.
• W2893530958 hasConcept C2778305200 @default.
• W2893530958 hasConcept C2780235182 @default.

• next