FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893560272> ?p ?o ?g. }

• W2893560272 endingPage "12" @default.
• W2893560272 startingPage "1" @default.
• W2893560272 abstract "Human papillomavirus (HPV) is the causative agent in genital warts and nearly all cervical, anogenital, and oropharyngeal cancers. Nine HPV types (6, 11, 16, 18, 31, 33, 45, 52, and 58) are associated with about 90% of cervical cancers and 90% of genital warts. HPV neutralization by vaccine-elicited neutralizing antibodies can block viral infection and prevent HPV-associated diseases. However, there is only one commercially available HPV vaccine, Gardasil 9, produced from Saccharomyces cerevisiae that covers all nine types, raising the need for microbial production of broad-spectrum HPV vaccines. Here, we investigated whether N-terminal truncations of the major HPV capsid proteins L1, improve their soluble expression in Escherichia coli. We found that N-terminal truncations promoted the soluble expression of HPV 33 (truncated by 10 amino acids [aa]), 52 (15 aa), and 58 (10 aa). The resultant HPV L1 proteins were purified in pentamer form and extensively characterized with biochemical, biophysical, and immunochemical methods. The pentamers self-assembled into virus-like particles (VLPs) in vitro, and 3D cryo-EM reconstructions revealed that all formed T = 7 icosahedral particles having 50-60-nm diameters. Moreover, we formulated a nine-valent HPV vaccine candidate with aluminum adjuvant and L1 VLPs from four genotypes used in this study and five from previous work. Immunogenicity assays in mice and non-human primates indicated that this HPV nine-valent vaccine candidate elicits neutralizing antibody titers comparable to those induced by Gardasil 9. Our study provides a method for producing a nine-valent HPV vaccine in E. coli and may inform strategies for the soluble expression of other vaccine candidates." @default.
• W2893560272 created "2018-10-05" @default.
• W2893560272 creator A5001993973 @default.
• W2893560272 creator A5010142228 @default.
• W2893560272 creator A5010156467 @default.
• W2893560272 creator A5010200561 @default.
• W2893560272 creator A5010898734 @default.
• W2893560272 creator A5021070038 @default.
• W2893560272 creator A5021892214 @default.
• W2893560272 creator A5024479009 @default.
• W2893560272 creator A5036121527 @default.
• W2893560272 creator A5047497897 @default.
• W2893560272 creator A5051434897 @default.
• W2893560272 creator A5053142224 @default.
• W2893560272 creator A5067654958 @default.
• W2893560272 creator A5068160427 @default.
• W2893560272 creator A5069610843 @default.
• W2893560272 creator A5071672663 @default.
• W2893560272 creator A5078732636 @default.
• W2893560272 date "2018-09-26" @default.
• W2893560272 modified "2023-10-14" @default.
• W2893560272 title "N-terminal truncations on L1 proteins of human papillomaviruses promote their soluble expression in <i>Escherichia coli</i> and self-assembly in vitro" @default.
• W2893560272 cites W1519578291 @default.
• W2893560272 cites W1808115300 @default.
• W2893560272 cites W1957151625 @default.
• W2893560272 cites W1967615961 @default.
• W2893560272 cites W1980193628 @default.
• W2893560272 cites W1981725818 @default.
• W2893560272 cites W1992291112 @default.
• W2893560272 cites W1996266688 @default.
• W2893560272 cites W1997991336 @default.
• W2893560272 cites W2001429203 @default.
• W2893560272 cites W2007816485 @default.
• W2893560272 cites W2008395860 @default.
• W2893560272 cites W2010311675 @default.
• W2893560272 cites W2012868689 @default.
• W2893560272 cites W2016217809 @default.
• W2893560272 cites W2032041326 @default.
• W2893560272 cites W2033105934 @default.
• W2893560272 cites W2033693020 @default.
• W2893560272 cites W2035185947 @default.
• W2893560272 cites W2038315892 @default.
• W2893560272 cites W2040428235 @default.
• W2893560272 cites W2046182783 @default.
• W2893560272 cites W2052933576 @default.
• W2893560272 cites W2056340694 @default.
• W2893560272 cites W2058967072 @default.
• W2893560272 cites W2062871072 @default.
• W2893560272 cites W2068900157 @default.
• W2893560272 cites W2070319921 @default.
• W2893560272 cites W2071976929 @default.
• W2893560272 cites W2074063726 @default.
• W2893560272 cites W2094377797 @default.
• W2893560272 cites W2100455255 @default.
• W2893560272 cites W2104234755 @default.
• W2893560272 cites W2106682089 @default.
• W2893560272 cites W2107391580 @default.
• W2893560272 cites W2113442300 @default.
• W2893560272 cites W2116827928 @default.
• W2893560272 cites W2119947460 @default.
• W2893560272 cites W2121335263 @default.
• W2893560272 cites W2123563358 @default.
• W2893560272 cites W2123853921 @default.
• W2893560272 cites W2126246455 @default.
• W2893560272 cites W2127169166 @default.
• W2893560272 cites W2130283750 @default.
• W2893560272 cites W2133525827 @default.
• W2893560272 cites W2139736159 @default.
• W2893560272 cites W2150088870 @default.
• W2893560272 cites W2154786226 @default.
• W2893560272 cites W2159650706 @default.
• W2893560272 cites W2159831861 @default.
• W2893560272 cites W2164149430 @default.
• W2893560272 cites W2348872694 @default.
• W2893560272 cites W240876023 @default.
• W2893560272 cites W2410044662 @default.
• W2893560272 cites W2570309823 @default.
• W2893560272 cites W2591847218 @default.
• W2893560272 cites W2611725828 @default.
• W2893560272 cites W2738852122 @default.
• W2893560272 cites W46899889 @default.
• W2893560272 doi "https://doi.org/10.1038/s41426-018-0158-2" @default.
• W2893560272 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6156512" @default.
• W2893560272 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30254257" @default.
• W2893560272 hasPublicationYear "2018" @default.
• W2893560272 type Work @default.
• W2893560272 sameAs 2893560272 @default.
• W2893560272 citedByCount "14" @default.
• W2893560272 countsByYear W28935602722020 @default.
• W2893560272 countsByYear W28935602722021 @default.
• W2893560272 countsByYear W28935602722022 @default.
• W2893560272 countsByYear W28935602722023 @default.
• W2893560272 crossrefType "journal-article" @default.
• W2893560272 hasAuthorship W2893560272A5001993973 @default.
• W2893560272 hasAuthorship W2893560272A5010142228 @default.
• W2893560272 hasAuthorship W2893560272A5010156467 @default.
• W2893560272 hasAuthorship W2893560272A5010200561 @default.
• W2893560272 hasAuthorship W2893560272A5010898734 @default.

• next