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• W2893582642 abstract "Abstract Explorative visualization techniques provide a first summary of microbiome read count datasets through dimension reduction. A plethora of dimension reduction methods exists, but many of them focus primarily on sample ordination, failing to elucidate the role of the bacterial species. Moreover, implicit but often unrealistic assumptions underlying these methods fail to account for overdispersion and differences in sequencing depth, which are two typical characteristics of sequencing data. We combine log-linear models with a dispersion estimation algorithm and flexible response function modelling into a framework for unconstrained and constrained ordination. The method allows easy filtering of technical confounders. As opposed to most existing ordination methods, the assumptions underlying the method are stated explicitly and can be verified using simple diagnostics. The combination of unconstrained and constrained ordination in the same framework is unique in the field and greatly facilitates microbiome data exploration. We illustrate the advantages of our method on simulated and real datasets, while pointing out flaws in existing methods. The algorithms for fitting and plotting are available in the R-package RCM ." @default.
• W2893582642 created "2018-10-05" @default.
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• W2893582642 date "2018-09-27" @default.
• W2893582642 modified "2023-10-18" @default.
• W2893582642 title "A unified framework for unconstrained and constrained ordination of microbiome read count data" @default.
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• W2893582642 doi "https://doi.org/10.1101/429340" @default.
• W2893582642 hasPublicationYear "2018" @default.
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