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- W2893685282 abstract "The purposes of this study were to analyze the atypical hemolysin gene of V. campbellii isolates, and to evaluate the effects of cultivation salinity (0.5%, 1.5%, and 3.0% NaCl) and pH (5.0, 7.3, and 8.6) on the hemolytic activity and virulence of V. campbellii . Phylogenetic analysis of atypical hemolysin gene sequences obtained from V. campbellii demonstrated 84%–85% identity with the hlyA of V. cholerae . V. campbellii grown at 1.5% or 3.0% NaCl exhibited significant higher hemolytic activity compared to those previously grown at 0.5% NaCl. Maximum hemolytic activity was observed among acid-adapted V. campbellii which was previously grown at pH 5.0 for 5 h, and its virulence against Galleria mellonella was enhanced (~20 times) in comparison to that of non-adapted V. campbellii . Based on our results, it seems that V. campbellii might have acquired hemolysin gene from V. cholerae . Moreover, cultivation salinity and pH is important for the hemolytic activity and virulence of V. campbellii . This will be useful for environmental control of this pathogen in aquaculture." @default.
- W2893685282 created "2018-10-05" @default.
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- W2893685282 date "2018-05-08" @default.
- W2893685282 modified "2023-09-27" @default.
- W2893685282 title "Phylogenetic analysis of atypical hemolysin gene in Vibrio campbellii and effects of cultivation salinity and pH on hemolytic activity and virulence" @default.
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