FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893693492> ?p ?o ?g. }

• W2893693492 endingPage "6165" @default.
• W2893693492 startingPage "6153" @default.
• W2893693492 abstract "Single and double tubulin rings were studied under a range of conditions and during microtubule (MT) assembly and disassembly. Here, tubulin was purified from porcine brain and used without any further modifications or additives that promote ring assembly. The structure of single GDP-rich tubulin rings was determined by cryo-transmission electron microscopy and synchrotron solution X-ray scattering. The scattering curves were fitted to atomic models, using our state-of-the-art analysis software, D+ . We found that there is a critical concentration for ring formation, which increased with GTP concentration with temperature. MT assembly or disassembly, induced by changes in temperature, was analyzed by time-resolved small-angle X-ray scattering. During MT assembly, the fraction of rings and unassembled dimers simultaneously decreased. During MT disassembly, the mass fraction of dimers increased. The increase in the concentration of rings was delayed until the fraction of dimers was sufficiently high. We verified that pure dimers, eluted via size-exclusion chromatography, could also form rings. Interestingly, X-ray radiation triggered tubulin ring disassembly. The concentration of disassembled rings versus exposure time followed a first-order kinetics. The disassembly rate constant and initial concentration were determined. X-ray radiation-triggered disassembly was used to determine the concentration of rings. We confirmed that following a temperature jump, the mass fraction of rings decreased and then stabilized at a constant value during the first stage of the MT assembly kinetics. This study sheds light on the most basic assembly and disassembly conditions for in vitro single GDP-rich tubulin rings and their relation to MT kinetics." @default.
• W2893693492 created "2018-10-05" @default.
• W2893693492 creator A5004675795 @default.
• W2893693492 creator A5004710745 @default.
• W2893693492 creator A5010603554 @default.
• W2893693492 creator A5032736688 @default.
• W2893693492 creator A5044585345 @default.
• W2893693492 date "2018-09-24" @default.
• W2893693492 modified "2023-10-03" @default.
• W2893693492 title "Structure, Assembly, and Disassembly of Tubulin Single Rings" @default.
• W2893693492 cites W1520923182 @default.
• W2893693492 cites W1749825239 @default.
• W2893693492 cites W1965196417 @default.
• W2893693492 cites W1965404941 @default.
• W2893693492 cites W1967664991 @default.
• W2893693492 cites W1970894830 @default.
• W2893693492 cites W1971913380 @default.
• W2893693492 cites W1975198943 @default.
• W2893693492 cites W1979783832 @default.
• W2893693492 cites W1981338907 @default.
• W2893693492 cites W1982531014 @default.
• W2893693492 cites W1983776866 @default.
• W2893693492 cites W1983874564 @default.
• W2893693492 cites W1997161926 @default.
• W2893693492 cites W2001719955 @default.
• W2893693492 cites W2004286543 @default.
• W2893693492 cites W2005845714 @default.
• W2893693492 cites W2006624308 @default.
• W2893693492 cites W2010246590 @default.
• W2893693492 cites W2012778514 @default.
• W2893693492 cites W2014528904 @default.
• W2893693492 cites W2014960196 @default.
• W2893693492 cites W2017986987 @default.
• W2893693492 cites W2019984193 @default.
• W2893693492 cites W2022993744 @default.
• W2893693492 cites W2027888342 @default.
• W2893693492 cites W2028127893 @default.
• W2893693492 cites W2033241989 @default.
• W2893693492 cites W2033790849 @default.
• W2893693492 cites W2036218826 @default.
• W2893693492 cites W2036982477 @default.
• W2893693492 cites W2037415133 @default.
• W2893693492 cites W2046975888 @default.
• W2893693492 cites W2047691221 @default.
• W2893693492 cites W2050690464 @default.
• W2893693492 cites W2057337811 @default.
• W2893693492 cites W2061637739 @default.
• W2893693492 cites W2062194766 @default.
• W2893693492 cites W2064571598 @default.
• W2893693492 cites W2066088601 @default.
• W2893693492 cites W2070395446 @default.
• W2893693492 cites W2071451843 @default.
• W2893693492 cites W2072986748 @default.
• W2893693492 cites W2087130779 @default.
• W2893693492 cites W2087738720 @default.
• W2893693492 cites W2089645884 @default.
• W2893693492 cites W2092281100 @default.
• W2893693492 cites W2093014273 @default.
• W2893693492 cites W2107301897 @default.
• W2893693492 cites W2112742525 @default.
• W2893693492 cites W2131837878 @default.
• W2893693492 cites W2147307619 @default.
• W2893693492 cites W2148557238 @default.
• W2893693492 cites W2149865591 @default.
• W2893693492 cites W2150576297 @default.
• W2893693492 cites W2162785195 @default.
• W2893693492 cites W2170344660 @default.
• W2893693492 cites W2300323866 @default.
• W2893693492 cites W2467701067 @default.
• W2893693492 cites W2487355739 @default.
• W2893693492 cites W2495353297 @default.
• W2893693492 cites W2496400485 @default.
• W2893693492 cites W2538434707 @default.
• W2893693492 cites W2615650441 @default.
• W2893693492 cites W2617612657 @default.
• W2893693492 cites W2747750550 @default.
• W2893693492 cites W2797907849 @default.
• W2893693492 cites W4293247451 @default.
• W2893693492 cites W2042910598 @default.
• W2893693492 doi "https://doi.org/10.1021/acs.biochem.8b00560" @default.
• W2893693492 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30247898" @default.
• W2893693492 hasPublicationYear "2018" @default.
• W2893693492 type Work @default.
• W2893693492 sameAs 2893693492 @default.
• W2893693492 citedByCount "16" @default.
• W2893693492 countsByYear W28936934922019 @default.
• W2893693492 countsByYear W28936934922020 @default.
• W2893693492 countsByYear W28936934922021 @default.
• W2893693492 countsByYear W28936934922022 @default.
• W2893693492 countsByYear W28936934922023 @default.
• W2893693492 crossrefType "journal-article" @default.
• W2893693492 hasAuthorship W2893693492A5004675795 @default.
• W2893693492 hasAuthorship W2893693492A5004710745 @default.
• W2893693492 hasAuthorship W2893693492A5010603554 @default.
• W2893693492 hasAuthorship W2893693492A5032736688 @default.
• W2893693492 hasAuthorship W2893693492A5044585345 @default.
• W2893693492 hasConcept C120665830 @default.
• W2893693492 hasConcept C121332964 @default.

• next