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• W2893759183 abstract "Abstract The kinetics of metabolic processes can be assessed, in real time by means of MR hyperpolarized (HP) metabolites. [1‐ 13 C]pyruvate, hyperpolarized by means of d‐DNP, is, by far, the substrate most widely applied to the investigation of several pathologies characterized by deregulated glycolytic metabolic networks, including cancer. Hyperpolarization of [1‐ 13 C]pyruvate by means of the cost effective, fast and easy to handle PHIP‐SAH (para‐hydrogen induced polarization‐side arm hydrogenation) method opens‐up a pathway for the application of HP metabolites to a wide range of cancer‐related studies. Herein, we report the first application of PHIP‐SAH hyperpolarized [1‐ 13 C]pyruvate in the investigation of upregulated glycolysis in two murine breast cancer cell lines (168FARN and 4T1). The results obtained using HP pyruvate have been validated with a conventional biochemical assay and are coherent with previously‐reported lactate dehydrogenase activity measured in those cells." @default.
• W2893759183 created "2018-10-05" @default.
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• W2893759183 date "2018-10-24" @default.
• W2893759183 modified "2023-10-10" @default.
• W2893759183 title "Metabolic Studies of Tumor Cells Using [1‐¹³C] Pyruvate Hyperpolarized by Means of PHIP‐Side Arm Hydrogenation" @default.
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• W2893759183 doi "https://doi.org/10.1002/cphc.201800652" @default.
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