FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893765475> ?p ?o ?g. }

• W2893765475 endingPage "349" @default.
• W2893765475 startingPage "335" @default.
• W2893765475 abstract "The extent and pattern of glycosylation on therapeutic antibodies can influence their circulatory half-life, engagement of effector functions, and immunogenicity, with direct consequences to efficacy and patient safety. Hence, controlling glycosylation patterns is central to any drug development program, yet poses a formidable challenge to the bio-manufacturing industry. Process changes, which can affect glycosylation patterns, range from manufacturing at different scales or sites, to switching production process mode, all the way to using alternative host cell lines. In the emerging space of biosimilars development, often times all of these aspects apply. Gaining a deep understanding of the direction and extent to which glycosylation quality attributes can be modulated is key for efficient fine-tuning of glycan profiles in a stage appropriate manner, but establishment of such platform knowledge is time consuming and resource intensive. Here we report an inexpensive and highly adaptable screening system for comprehensive modulation of glycans on antibodies expressed in CHO cells. We characterize 10 media additives in univariable studies and in combination, using a design of experiments approach to map the design space for tuning glycosylation profile attributes. We introduce a robust workflow that does not require automation, yet enables rapid process optimization. We demonstrate scalability across deep wells, shake flasks, AMBR-15 cell culture system, and 2 L single-use bioreactors. Further, we show that it is broadly applicable to different molecules and host cell lineages. This universal approach permits fine-tuned modulation of glycan product quality, reduces development costs, and enables agile implementation of process changes throughout the product lifecycle." @default.
• W2893765475 created "2018-10-05" @default.
• W2893765475 creator A5001685069 @default.
• W2893765475 creator A5005576534 @default.
• W2893765475 creator A5022750424 @default.
• W2893765475 creator A5037235488 @default.
• W2893765475 creator A5037875294 @default.
• W2893765475 creator A5047433088 @default.
• W2893765475 creator A5050948603 @default.
• W2893765475 creator A5083421500 @default.
• W2893765475 date "2018-10-22" @default.
• W2893765475 modified "2023-09-27" @default.
• W2893765475 title "Comprehensive manipulation of glycosylation profiles across development scales" @default.
• W2893765475 cites W1522253950 @default.
• W2893765475 cites W1576401981 @default.
• W2893765475 cites W1636483053 @default.
• W2893765475 cites W1686214102 @default.
• W2893765475 cites W1886156337 @default.
• W2893765475 cites W1965185630 @default.
• W2893765475 cites W1982056508 @default.
• W2893765475 cites W2006994535 @default.
• W2893765475 cites W2010385141 @default.
• W2893765475 cites W2018533661 @default.
• W2893765475 cites W2018795884 @default.
• W2893765475 cites W2027894892 @default.
• W2893765475 cites W2034450975 @default.
• W2893765475 cites W2036076292 @default.
• W2893765475 cites W2059157822 @default.
• W2893765475 cites W2065419141 @default.
• W2893765475 cites W2071888779 @default.
• W2893765475 cites W2081854754 @default.
• W2893765475 cites W2083233483 @default.
• W2893765475 cites W2083441961 @default.
• W2893765475 cites W2084136419 @default.
• W2893765475 cites W2089927923 @default.
• W2893765475 cites W2097445454 @default.
• W2893765475 cites W2106325741 @default.
• W2893765475 cites W2110395829 @default.
• W2893765475 cites W2124847934 @default.
• W2893765475 cites W2131231783 @default.
• W2893765475 cites W2134074056 @default.
• W2893765475 cites W2134518608 @default.
• W2893765475 cites W2137354517 @default.
• W2893765475 cites W2146981871 @default.
• W2893765475 cites W2155819450 @default.
• W2893765475 cites W2157200718 @default.
• W2893765475 cites W2161698546 @default.
• W2893765475 cites W2162406947 @default.
• W2893765475 cites W2165567653 @default.
• W2893765475 cites W2306423776 @default.
• W2893765475 cites W2380172638 @default.
• W2893765475 cites W2516808437 @default.
• W2893765475 cites W2588197869 @default.
• W2893765475 doi "https://doi.org/10.1080/19420862.2018.1527665" @default.
• W2893765475 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6380396" @default.
• W2893765475 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30252592" @default.
• W2893765475 hasPublicationYear "2018" @default.
• W2893765475 type Work @default.
• W2893765475 sameAs 2893765475 @default.
• W2893765475 citedByCount "12" @default.
• W2893765475 countsByYear W28937654752019 @default.
• W2893765475 countsByYear W28937654752020 @default.
• W2893765475 countsByYear W28937654752021 @default.
• W2893765475 countsByYear W28937654752022 @default.
• W2893765475 countsByYear W28937654752023 @default.
• W2893765475 crossrefType "journal-article" @default.
• W2893765475 hasAuthorship W2893765475A5001685069 @default.
• W2893765475 hasAuthorship W2893765475A5005576534 @default.
• W2893765475 hasAuthorship W2893765475A5022750424 @default.
• W2893765475 hasAuthorship W2893765475A5037235488 @default.
• W2893765475 hasAuthorship W2893765475A5037875294 @default.
• W2893765475 hasAuthorship W2893765475A5047433088 @default.
• W2893765475 hasAuthorship W2893765475A5050948603 @default.
• W2893765475 hasAuthorship W2893765475A5083421500 @default.
• W2893765475 hasBestOaLocation W28937654751 @default.
• W2893765475 hasConcept C108625454 @default.
• W2893765475 hasConcept C111919701 @default.
• W2893765475 hasConcept C127413603 @default.
• W2893765475 hasConcept C139807058 @default.
• W2893765475 hasConcept C150903083 @default.
• W2893765475 hasConcept C153911025 @default.
• W2893765475 hasConcept C169760540 @default.
• W2893765475 hasConcept C183696295 @default.
• W2893765475 hasConcept C204320433 @default.
• W2893765475 hasConcept C206212055 @default.
• W2893765475 hasConcept C206345919 @default.
• W2893765475 hasConcept C21547014 @default.
• W2893765475 hasConcept C2776207758 @default.
• W2893765475 hasConcept C2777313579 @default.
• W2893765475 hasConcept C2780449318 @default.
• W2893765475 hasConcept C31258907 @default.
• W2893765475 hasConcept C41008148 @default.
• W2893765475 hasConcept C48044578 @default.
• W2893765475 hasConcept C55493867 @default.
• W2893765475 hasConcept C59491497 @default.
• W2893765475 hasConcept C70721500 @default.
• W2893765475 hasConcept C77088390 @default.
• W2893765475 hasConcept C86803240 @default.

• next