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- W2893777927 abstract "Introduction Placenta-specific miRNAs play important role in the regulation of pregnancy, and altered miRNA expression is associated with preeclampsia (PE). In PE, the placental dysfunction causes the abnormal release of extracellular miRNAs to the maternal circulation. These are highly stable, being encapsulated inside vesicles (e.g. exosomes) or bound to Argonaute (Ago) proteins. Previously we showed that the hypoxia-sensitive hsa-mir-210 is elevated in exosome fractions in case of PE. Objective Our aim was to analyze the expression of miR-210 in placenta, exosome and Ago-bound fractions comparing normal (N) and PE pregnancies. We investigated the Ago-bound/exosomal miRNA ratio and compared the expression profiles of the two fractions to the placental miRNA expression. Methods PE and N placenta samples were collected from C-sections and blood samples were drawn from each pregnant in the third trimester. Plasma was separated, and exosomes were isolated by membrane affinity spin column method. Short RNAs were extracted from exosomes and vesicle-free fractions, and total-RNA was isolated from the placenta samples. Expression analysis was carried out by qPCR with specific primers to target and reference miRNAs. Results The level of placental miR-210 was significantly higher in affected samples versus the control group. We found that miR-210 was overrepresented in the Ago-bound fractions compared to exosomal fractions in both groups. The ratio of Ago-bound/exosomal miR-210 was relatively higher in PE compared to N group. The placental miR-210 excess in PE samples could be associated with higher levels of miR-210 in the Ago-fraction. Discussion We investigated placental, exosomal and Ago-bound miR-210 expression profile in PE vs N groups. The level of miR-210 was significantly higher in PE placentas, which could be related to the increase of circulating Ago-bound miR-210. In PE, apart from active exosomal secretion, there is also a large-scale passive release of Ago-bound miRNAs." @default.
- W2893777927 created "2018-10-05" @default.
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- W2893777927 date "2018-10-01" @default.
- W2893777927 modified "2023-10-18" @default.
- W2893777927 title "194. Connections between circulating argonaute-bound, exosomal and placental miR-210 expression patterns in preeclampsia" @default.
- W2893777927 doi "https://doi.org/10.1016/j.preghy.2018.08.090" @default.
- W2893777927 hasPublicationYear "2018" @default.
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