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- W2893787568 abstract "人类表皮生长因子受体2（Human Epidermal Growth Factor Receptor 2，HER-2）是乳腺癌最重要的致病基因。本文探讨131Ⅰ标记的HER-2特异结合小分子模拟肽B2-S22-AFA对乳腺癌细胞的特异性杀伤作用。采用溴代琥珀酰亚胺法（N-Bromide Succinimide，NBS）进行131Ⅰ标记B2-S22-AFA，测定标记率、放化纯度、稳定性及免疫活性。用Western Blot法和免疫组化法测定人乳腺癌SKBR3及MDA-MB-231Her-2细胞HER-2表达，观察不同浓度表皮生长因子（Epidermal Growth Factor，EGF）条件下B2-S22-AFA对细胞生长的抑制率。设B2-S22-AFA组（B2-S22-AFA终浓度1 μg·mL-1）、131Ⅰ-B2-S22-AFA组（131Ⅰ-B2-S22-AFA终浓度144 kBq·μg-1·mL）、131Ⅰ组（131Ⅰ终浓度144 kBq·mL-1）、阴性对照组及试剂空白组，每组EGF终浓度均5.0 pg·mL-1，采用四唑盐比色法（Methyl Thiazolyl Tetrazolium，MTT）测定SKBR3和MDA-MB-231细胞增殖活性，观察131Ⅰ-B2-S22-AFA、B2-S22-AFA及131Ⅰ作用不同时间对两种细胞生长的抑制率。结果显示，HER-2在SKBR3细胞呈强阳性表达，在MDA-MB-231细胞呈弱（或低）表达。131Ⅰ-B2-S22-AFA标记率、放化纯度及比活度分别为64.8%-79.6%、95.0%-97.6%、151.7 MBq·mg-1，在37℃血清中放置4 h、12 h、24 h、48 h、72 h的放化纯度分别95.4%、93.5%、91.4%、88.2%、77.4%，131Ⅰ-B2-S22-AFA与SKBR3细胞及MDA-MB-231细胞的最大结合率分别（66.47±3.24）%和（3.89±0.81）%（3次）。当EGF存在时，B2-S22-AFA可明显抑制SKBR3细胞生长；无EGF时，B2-S22-AFA无此作用。131Ⅰ-B2-S22-AFA对SKBR3细胞的杀伤作用较B2-S22-AFA和131Ⅰ明显增强（均p < 0.05），B2-S22-AFA对SKBR3细胞杀伤作用显著高于131Ⅰ（p < 0.05），131Ⅰ-B2-S22-AFA和B2-S22-AFA对MDA-MB-231细胞均无杀伤作用。综上所述，131Ⅰ-B2-S22-AFA可显著加强、加速B2-S22-AFA对HER-2高表达乳腺癌细胞的特异性杀伤作用。" @default.
- W2893787568 created "2018-10-05" @default.
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- W2893787568 date "2017-12-08" @default.
- W2893787568 modified "2023-09-23" @default.
- W2893787568 title "131^I-B2-S22-AFA对HER-2高表达乳腺癌细胞的杀伤作用" @default.
- W2893787568 doi "https://doi.org/10.11889/j.0253-3219.2017.hjs.40.120301" @default.
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