FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893792076> ?p ?o ?g. }

• W2893792076 abstract "TGF-β promotes tumor invasion and metastasis through inducing epithelial-mesenchymal transition (EMT) in non-small cell lung cancer (NSCLC). Circular RNAs (circRNAs) are recognized as functional non-coding RNAs involved in human cancers. However, whether and how circRNAs contribute to TGF-β-induced EMT and metastasis in NSCLC remain vague. Here, we investigated the regulation and function of Circular RNA hsa_circ_0008305 (circPTK2) in TGF-β-induced EMT and tumor metastasis, as well as a link between circPTK2 and transcriptional intermediary factor 1 γ (TIF1γ) in NSCLC.Circular RNAs were determined by human circRNA Array analysis, real-time quantitative reverse transcriptase PCR and northern blot. Luciferase reporter, RNA-binding protein immunoprecipitation (RIP), RNA pull-down and fluorescence in situ hybridization (FISH) assays were employed to test the interaction between circPTK2 and miR-429/miR-200b-3p. Ectopic overexpression and siRNA-mediated knockdown of circPTK2, TGF-β-induced EMT, Transwell migration and invasion in vitro, and in vivo experiment of metastasis were used to evaluate the function of circPTK2. Transcription and prognosis analyses were done in public databases.CircPTK2 and TIF1γ were significantly down-regulated in NSCLC cells undergoing EMT induced by TGF-β. CircPTK2 overexpression augmented TIF1γ expression, inhibited TGF-β-induced EMT and NSCLC cell invasion, whereas circPTK2 knockdown had the opposite effects. CircPTK2 functions as a sponge of miR-429/miR-200b-3p, and miR-429/miR-200b-3p promote TGF-β-induced EMT and NSCLC cell invasion by targeting TIF1γ. CircPTK2 overexpression inhibited the invasion-promoting phenotype of endogenous miR-429/miR-200b-3p in NSCLC cells in response to TGF-β. CircPTK2 overexpression significantly decreased the expression of Snail, an important downstream transcriptional activator of TGF-β/Smad signaling. In an in vivo experiment of metastasis, circPTK2 overexpression suppressed NSCLC cell metastasis. Moreover, circPTK2 expression was dramatically down-regulated and positively correlated with TIF1γ expression in human NSCLC tissues. Especially, circPTK2 was significantly lower in metastatic NSCLC tissues than non-metastatic counterparts.Our findings show that circPTK2 (hsa_circ_0008305) inhibits TGF-β-induced EMT and metastasis by controlling TIF1γ in NSCLC, revealing a novel mechanism by which circRNA regulates TGF-β-induced EMT and tumor metastasis, and suggesting that circPTK2 overexpression could provide a therapeutic strategy for advanced NSCLC." @default.
• W2893792076 created "2018-10-05" @default.
• W2893792076 creator A5023826377 @default.
• W2893792076 creator A5028401090 @default.
• W2893792076 creator A5030635953 @default.
• W2893792076 creator A5031355441 @default.
• W2893792076 creator A5033113336 @default.
• W2893792076 creator A5034266315 @default.
• W2893792076 creator A5045912055 @default.
• W2893792076 creator A5047427786 @default.
• W2893792076 creator A5051624923 @default.
• W2893792076 creator A5054656489 @default.
• W2893792076 creator A5062293752 @default.
• W2893792076 creator A5069334991 @default.
• W2893792076 creator A5084024908 @default.
• W2893792076 creator A5084539249 @default.
• W2893792076 creator A5091553972 @default.
• W2893792076 date "2018-09-27" @default.
• W2893792076 modified "2023-10-14" @default.
• W2893792076 title "Circular RNA hsa_circ_0008305 (circPTK2) inhibits TGF-β-induced epithelial-mesenchymal transition and metastasis by controlling TIF1γ in non-small cell lung cancer" @default.
• W2893792076 cites W1543571147 @default.
• W2893792076 cites W1966557314 @default.
• W2893792076 cites W1966701340 @default.
• W2893792076 cites W1970370172 @default.
• W2893792076 cites W1974606154 @default.
• W2893792076 cites W1977957483 @default.
• W2893792076 cites W1995778320 @default.
• W2893792076 cites W1998561093 @default.
• W2893792076 cites W2020848957 @default.
• W2893792076 cites W2028642248 @default.
• W2893792076 cites W2032114357 @default.
• W2893792076 cites W2034305079 @default.
• W2893792076 cites W2050471794 @default.
• W2893792076 cites W2053791813 @default.
• W2893792076 cites W2058835062 @default.
• W2893792076 cites W2060940710 @default.
• W2893792076 cites W2064165694 @default.
• W2893792076 cites W2068517905 @default.
• W2893792076 cites W2075231419 @default.
• W2893792076 cites W2083891812 @default.
• W2893792076 cites W2086750497 @default.
• W2893792076 cites W2093921289 @default.
• W2893792076 cites W2101136328 @default.
• W2893792076 cites W2107148940 @default.
• W2893792076 cites W2110272715 @default.
• W2893792076 cites W2116822103 @default.
• W2893792076 cites W2127658932 @default.
• W2893792076 cites W2131648467 @default.
• W2893792076 cites W2132260682 @default.
• W2893792076 cites W2136280424 @default.
• W2893792076 cites W2136741329 @default.
• W2893792076 cites W2137373909 @default.
• W2893792076 cites W2140108163 @default.
• W2893792076 cites W2156629568 @default.
• W2893792076 cites W2235523093 @default.
• W2893792076 cites W2266356262 @default.
• W2893792076 cites W2272984102 @default.
• W2893792076 cites W2344312120 @default.
• W2893792076 cites W2364988192 @default.
• W2893792076 cites W2408306952 @default.
• W2893792076 cites W2413429676 @default.
• W2893792076 cites W2476707374 @default.
• W2893792076 cites W2510965737 @default.
• W2893792076 cites W2517943968 @default.
• W2893792076 cites W2519661094 @default.
• W2893792076 cites W2521514890 @default.
• W2893792076 cites W2573032256 @default.
• W2893792076 cites W2591328939 @default.
• W2893792076 cites W2593315117 @default.
• W2893792076 cites W2602342670 @default.
• W2893792076 cites W2616072553 @default.
• W2893792076 cites W2767999015 @default.
• W2893792076 cites W365311518 @default.
• W2893792076 doi "https://doi.org/10.1186/s12943-018-0889-7" @default.
• W2893792076 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6161470" @default.
• W2893792076 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30261900" @default.
• W2893792076 hasPublicationYear "2018" @default.
• W2893792076 type Work @default.
• W2893792076 sameAs 2893792076 @default.
• W2893792076 citedByCount "242" @default.
• W2893792076 countsByYear W28937920762019 @default.
• W2893792076 countsByYear W28937920762020 @default.
• W2893792076 countsByYear W28937920762021 @default.
• W2893792076 countsByYear W28937920762022 @default.
• W2893792076 countsByYear W28937920762023 @default.
• W2893792076 crossrefType "journal-article" @default.
• W2893792076 hasAuthorship W2893792076A5023826377 @default.
• W2893792076 hasAuthorship W2893792076A5028401090 @default.
• W2893792076 hasAuthorship W2893792076A5030635953 @default.
• W2893792076 hasAuthorship W2893792076A5031355441 @default.
• W2893792076 hasAuthorship W2893792076A5033113336 @default.
• W2893792076 hasAuthorship W2893792076A5034266315 @default.
• W2893792076 hasAuthorship W2893792076A5045912055 @default.
• W2893792076 hasAuthorship W2893792076A5047427786 @default.
• W2893792076 hasAuthorship W2893792076A5051624923 @default.
• W2893792076 hasAuthorship W2893792076A5054656489 @default.
• W2893792076 hasAuthorship W2893792076A5062293752 @default.
• W2893792076 hasAuthorship W2893792076A5069334991 @default.
• W2893792076 hasAuthorship W2893792076A5084024908 @default.
• W2893792076 hasAuthorship W2893792076A5084539249 @default.

• next