FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893792887> ?p ?o ?g. }

• W2893792887 endingPage "2175" @default.
• W2893792887 startingPage "2149" @default.
• W2893792887 abstract "Protein depletion is a key approach to understanding the functions of a protein in a biological system. We recently developed the Trim-Away approach in order to rapidly degrade endogenous proteins without prior modification. Trim-Away is based on the ubiquitin ligase and Fc receptor TRIM21, which recognizes antibody-bound proteins and targets them for degradation by the proteasome. In a typical Trim-Away experiment, protein degradation is achieved in three steps: first, introduction of an antibody against the target protein; second, recruitment of endogenous or exogenous/overexpressed TRIM21 to the antibody–bound target protein; and third, proteasome-mediated degradation of the target protein, antibody and TRIM21 complex. Protein degradation by Trim-Away is acute and rapid, with half-lives of ~10–20 min. The major advantages of Trim-Away over other protein degradation methods are that it can be applied to any endogenous protein without prior modification; that it uses conventional antibodies that are widely available; and that it can be applied to a wide range of cell types, including nondividing primary human cells, for which other loss-of-function assays are challenging. In this protocol, we describe the detailed procedures for antibody preparation and delivery in mouse oocytes and cultured cells via microinjection and electroporation. In addition, we provide recommendations for antibody selection and validation, and for the generation of TRIM21-overexpressing cell lines for cases in which endogenous TRIM21 is limited. A typical Trim-Away experiment takes just a few hours. This protocol describes Trim-Away, an approach for rapid protein depletion in different cell types. TRIM21–mediated proteasomal degradation is induced by microinjection or electroporation of an antibody into the protein of interest." @default.
• W2893792887 created "2018-10-05" @default.
• W2893792887 creator A5029489083 @default.
• W2893792887 creator A5060600351 @default.
• W2893792887 creator A5065552925 @default.
• W2893792887 creator A5075832070 @default.
• W2893792887 creator A5089787029 @default.
• W2893792887 date "2018-09-24" @default.
• W2893792887 modified "2023-10-16" @default.
• W2893792887 title "Acute and rapid degradation of endogenous proteins by Trim-Away" @default.
• W2893792887 cites W1486227004 @default.
• W2893792887 cites W1563836657 @default.
• W2893792887 cites W1912867168 @default.
• W2893792887 cites W1950598818 @default.
• W2893792887 cites W1963722760 @default.
• W2893792887 cites W1965813899 @default.
• W2893792887 cites W1967761542 @default.
• W2893792887 cites W1971889411 @default.
• W2893792887 cites W1975035163 @default.
• W2893792887 cites W1979500993 @default.
• W2893792887 cites W1982166691 @default.
• W2893792887 cites W1982281628 @default.
• W2893792887 cites W1995701587 @default.
• W2893792887 cites W2009635392 @default.
• W2893792887 cites W2014557438 @default.
• W2893792887 cites W2018983969 @default.
• W2893792887 cites W2022754521 @default.
• W2893792887 cites W2023482962 @default.
• W2893792887 cites W2052172534 @default.
• W2893792887 cites W2052225170 @default.
• W2893792887 cites W2079411392 @default.
• W2893792887 cites W2079921190 @default.
• W2893792887 cites W2112444085 @default.
• W2893792887 cites W2120439612 @default.
• W2893792887 cites W2124833370 @default.
• W2893792887 cites W2132613389 @default.
• W2893792887 cites W2151683271 @default.
• W2893792887 cites W2294015155 @default.
• W2893792887 cites W2327864955 @default.
• W2893792887 cites W2415768383 @default.
• W2893792887 cites W2508113088 @default.
• W2893792887 cites W2525306762 @default.
• W2893792887 cites W2556650563 @default.
• W2893792887 cites W2563169901 @default.
• W2893792887 cites W2704517448 @default.
• W2893792887 cites W2735720719 @default.
• W2893792887 cites W2769995426 @default.
• W2893792887 cites W2790814744 @default.
• W2893792887 cites W805741100 @default.
• W2893792887 doi "https://doi.org/10.1038/s41596-018-0028-3" @default.
• W2893792887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30250286" @default.
• W2893792887 hasPublicationYear "2018" @default.
• W2893792887 type Work @default.
• W2893792887 sameAs 2893792887 @default.
• W2893792887 citedByCount "93" @default.
• W2893792887 countsByYear W28937928872019 @default.
• W2893792887 countsByYear W28937928872020 @default.
• W2893792887 countsByYear W28937928872021 @default.
• W2893792887 countsByYear W28937928872022 @default.
• W2893792887 countsByYear W28937928872023 @default.
• W2893792887 crossrefType "journal-article" @default.
• W2893792887 hasAuthorship W2893792887A5029489083 @default.
• W2893792887 hasAuthorship W2893792887A5060600351 @default.
• W2893792887 hasAuthorship W2893792887A5065552925 @default.
• W2893792887 hasAuthorship W2893792887A5075832070 @default.
• W2893792887 hasAuthorship W2893792887A5089787029 @default.
• W2893792887 hasBestOaLocation W28937928872 @default.
• W2893792887 hasConcept C104317684 @default.
• W2893792887 hasConcept C111919701 @default.
• W2893792887 hasConcept C134459356 @default.
• W2893792887 hasConcept C159654299 @default.
• W2893792887 hasConcept C16613235 @default.
• W2893792887 hasConcept C203014093 @default.
• W2893792887 hasConcept C25602115 @default.
• W2893792887 hasConcept C27740335 @default.
• W2893792887 hasConcept C2992195973 @default.
• W2893792887 hasConcept C41008148 @default.
• W2893792887 hasConcept C55493867 @default.
• W2893792887 hasConcept C86803240 @default.
• W2893792887 hasConcept C88611116 @default.
• W2893792887 hasConcept C95444343 @default.
• W2893792887 hasConceptScore W2893792887C104317684 @default.
• W2893792887 hasConceptScore W2893792887C111919701 @default.
• W2893792887 hasConceptScore W2893792887C134459356 @default.
• W2893792887 hasConceptScore W2893792887C159654299 @default.
• W2893792887 hasConceptScore W2893792887C16613235 @default.
• W2893792887 hasConceptScore W2893792887C203014093 @default.
• W2893792887 hasConceptScore W2893792887C25602115 @default.
• W2893792887 hasConceptScore W2893792887C27740335 @default.
• W2893792887 hasConceptScore W2893792887C2992195973 @default.
• W2893792887 hasConceptScore W2893792887C41008148 @default.
• W2893792887 hasConceptScore W2893792887C55493867 @default.
• W2893792887 hasConceptScore W2893792887C86803240 @default.
• W2893792887 hasConceptScore W2893792887C88611116 @default.
• W2893792887 hasConceptScore W2893792887C95444343 @default.
• W2893792887 hasIssue "10" @default.
• W2893792887 hasLocation W28937928871 @default.
• W2893792887 hasLocation W28937928872 @default.

• next