FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893800126> ?p ?o ?g. }

• W2893800126 endingPage "86" @default.
• W2893800126 startingPage "72" @default.
• W2893800126 abstract "Elevation of Ca2+i and AMP-activated protein kinase (AMPK) are considered as major signals triggering transcriptomic changes in exercising skeletal muscle. Electrical pulse stimulation (EPS) of cultured myotubes is widely employed as an in vitro model of muscle contraction. This study examines the impact of Ca2+i-mediated and Ca2+i-independent signaling in transcriptomic changes in EPS-treated C2C12 myotubes. Electrical pulse stimulation (40 V, 1 Hz, 10 ms, 2 h) resulted in [Ca2+]i oscillations, gain of Na+i, loss of K+i, and differential expression of 3215 transcripts. Additions of 10 μM nicardipine abolished [Ca2+]i oscillations but did not affect elevation of the [Na+]i/[K+]i ratio seen in EPS-treated myotubes. Differential expression of 1018 transcripts was preserved in the presence of nicardipine, indicating a Ca2+i-independent mechanism of excitation–transcription coupling. Among nicardipine-resistant transcripts, we noted 113 transcripts whose expression was also affected by partial Na+,K+-ATPase inhibition with 30 μM ouabain providing the same elevation of the [Na+]i/[K+]i ratio as in EPS-treated cells. Electrical pulse stimulation increased phosphorylation of CREB, ATF-1, Akt, ERK, and p38 MAPK without any impact on phosphorylation of acetyl-CoA carboxylase and Unc-51 like autophagy activating kinase-1, i.e. downstream markers of AMPK activation. Unlike CREB, ATF-1, and MAPKs, an increment in Akt phosphorylation was abolished by nicardipine. Thus, our results show that Ca2+i-independent signaling plays a key role in altered expression of 30% of studied genes in EPS-treated myotubes. This signaling pathway is at least partially triggered by dissipation of transmembrane gradients of monovalent cations." @default.
• W2893800126 created "2018-10-05" @default.
• W2893800126 creator A5019780697 @default.
• W2893800126 creator A5031351161 @default.
• W2893800126 creator A5053115271 @default.
• W2893800126 creator A5058775249 @default.
• W2893800126 creator A5068046329 @default.
• W2893800126 creator A5077948097 @default.
• W2893800126 creator A5081304319 @default.
• W2893800126 date "2018-12-01" @default.
• W2893800126 modified "2023-10-01" @default.
• W2893800126 title "Transcriptomic changes in C2C12 myotubes triggered by electrical stimulation: Role of Ca2+i-mediated and Ca2+i-independent signaling and elevated [Na+]i/[K+]i ratio" @default.
• W2893800126 cites W1515599953 @default.
• W2893800126 cites W1532212199 @default.
• W2893800126 cites W1775749144 @default.
• W2893800126 cites W1899877571 @default.
• W2893800126 cites W1964287631 @default.
• W2893800126 cites W1970205234 @default.
• W2893800126 cites W1970706920 @default.
• W2893800126 cites W1970757672 @default.
• W2893800126 cites W1971075258 @default.
• W2893800126 cites W1981932957 @default.
• W2893800126 cites W1983762553 @default.
• W2893800126 cites W1984582730 @default.
• W2893800126 cites W1985534077 @default.
• W2893800126 cites W1987938334 @default.
• W2893800126 cites W1989069558 @default.
• W2893800126 cites W1990374554 @default.
• W2893800126 cites W1998789906 @default.
• W2893800126 cites W1999175221 @default.
• W2893800126 cites W2000200743 @default.
• W2893800126 cites W2001096716 @default.
• W2893800126 cites W2001380129 @default.
• W2893800126 cites W2002456666 @default.
• W2893800126 cites W2006792851 @default.
• W2893800126 cites W2010394923 @default.
• W2893800126 cites W2017363474 @default.
• W2893800126 cites W2023772348 @default.
• W2893800126 cites W2035188247 @default.
• W2893800126 cites W2035768382 @default.
• W2893800126 cites W2036709958 @default.
• W2893800126 cites W2042791996 @default.
• W2893800126 cites W2044172090 @default.
• W2893800126 cites W2061075485 @default.
• W2893800126 cites W2062221551 @default.
• W2893800126 cites W2066461638 @default.
• W2893800126 cites W2066806510 @default.
• W2893800126 cites W2067406229 @default.
• W2893800126 cites W2069295886 @default.
• W2893800126 cites W2070962873 @default.
• W2893800126 cites W2071655334 @default.
• W2893800126 cites W2072586692 @default.
• W2893800126 cites W2079580613 @default.
• W2893800126 cites W2082584115 @default.
• W2893800126 cites W2084107123 @default.
• W2893800126 cites W2084868341 @default.
• W2893800126 cites W2088924661 @default.
• W2893800126 cites W2090386198 @default.
• W2893800126 cites W2091561553 @default.
• W2893800126 cites W2099699826 @default.
• W2893800126 cites W2100837269 @default.
• W2893800126 cites W2101438761 @default.
• W2893800126 cites W2102776209 @default.
• W2893800126 cites W2103488083 @default.
• W2893800126 cites W2104504959 @default.
• W2893800126 cites W2113056652 @default.
• W2893800126 cites W2115357041 @default.
• W2893800126 cites W2124102100 @default.
• W2893800126 cites W2129787631 @default.
• W2893800126 cites W2135931556 @default.
• W2893800126 cites W2139848693 @default.
• W2893800126 cites W2140700842 @default.
• W2893800126 cites W2147253226 @default.
• W2893800126 cites W2151319079 @default.
• W2893800126 cites W2155243614 @default.
• W2893800126 cites W2158794894 @default.
• W2893800126 cites W2161440135 @default.
• W2893800126 cites W2162019453 @default.
• W2893800126 cites W2168299580 @default.
• W2893800126 cites W2169857330 @default.
• W2893800126 cites W2171619592 @default.
• W2893800126 cites W2186127765 @default.
• W2893800126 cites W2218142692 @default.
• W2893800126 cites W2262649717 @default.
• W2893800126 cites W2264908888 @default.
• W2893800126 cites W227933217 @default.
• W2893800126 cites W2328108810 @default.
• W2893800126 cites W2529158368 @default.
• W2893800126 cites W2597912532 @default.
• W2893800126 cites W2606513998 @default.
• W2893800126 cites W2610112373 @default.
• W2893800126 cites W2760197529 @default.
• W2893800126 cites W2763401926 @default.
• W2893800126 cites W765287395 @default.
• W2893800126 doi "https://doi.org/10.1016/j.ceca.2018.09.007" @default.
• W2893800126 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30300758" @default.
• W2893800126 hasPublicationYear "2018" @default.
• W2893800126 type Work @default.

• next