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- W2893835395 abstract "Complexins play a critical role in the regulation of neurotransmission by regulating SNARE-mediated exocytosis of synaptic vesicles. Complexins can exert either a facilitatory or an inhibitory effect on neurotransmitter release, depending on the context, and different complexin domains contribute differently to these opposing roles. Structural characterization of the central helix domain of complexin bound to the assembled SNARE bundle provided key insights into the functional mechanism of this domain of complexin, which is critical for both complexin activities, but many questions remain, particularly regarding the roles and mechanisms of other complexin domains. Recent progress has clarified the structural properties of these additional domains, and has led to various proposals regarding how they contribute to complexin function. This chapter describes spectroscopic approaches used in our laboratory and others, primarily involving circular dichroism and solution-state NMR spectroscopy, to characterize structure within complexins when isolated or when bound to interaction partners. The ability to characterize complexin structure enables structure/function studies employing in vitro or in vivo assays of complexin function. More generally, these types of approaches can be used to study the binding of other intrinsically disordered proteins or protein regions to membrane surfaces or for that matter to other large physiological binding partners." @default.
- W2893835395 created "2018-10-05" @default.
- W2893835395 creator A5021363544 @default.
- W2893835395 creator A5023661358 @default.
- W2893835395 date "2018-01-01" @default.
- W2893835395 modified "2023-09-30" @default.
- W2893835395 title "Spectroscopic Characterization of Structure–Function Relationships in the Intrinsically Disordered Protein Complexin" @default.
- W2893835395 cites W1067583755 @default.
- W2893835395 cites W1498088837 @default.
- W2893835395 cites W1617965928 @default.
- W2893835395 cites W1916164999 @default.
- W2893835395 cites W1926834468 @default.
- W2893835395 cites W1965345344 @default.
- W2893835395 cites W1965659132 @default.
- W2893835395 cites W1970100121 @default.
- W2893835395 cites W1971509671 @default.
- W2893835395 cites W1972788996 @default.
- W2893835395 cites W1973361899 @default.
- W2893835395 cites W1973598302 @default.
- W2893835395 cites W1973980288 @default.
- W2893835395 cites W1975542945 @default.
- W2893835395 cites W1975759605 @default.
- W2893835395 cites W1976479871 @default.
- W2893835395 cites W1979535959 @default.
- W2893835395 cites W1980955669 @default.
- W2893835395 cites W1981063800 @default.
- W2893835395 cites W1983544779 @default.
- W2893835395 cites W1984960998 @default.
- W2893835395 cites W1985321331 @default.
- W2893835395 cites W1986469135 @default.
- W2893835395 cites W1986777984 @default.
- W2893835395 cites W1988903945 @default.
- W2893835395 cites W1991546697 @default.
- W2893835395 cites W1993018794 @default.
- W2893835395 cites W1993795908 @default.
- W2893835395 cites W1993958798 @default.
- W2893835395 cites W1997980735 @default.
- W2893835395 cites W1999702498 @default.
- W2893835395 cites W2001842463 @default.
- W2893835395 cites W2002768329 @default.
- W2893835395 cites W2008612498 @default.
- W2893835395 cites W2009639277 @default.
- W2893835395 cites W2013341912 @default.
- W2893835395 cites W2014299620 @default.
- W2893835395 cites W2014550271 @default.
- W2893835395 cites W2017924208 @default.
- W2893835395 cites W2017984279 @default.
- W2893835395 cites W2017999215 @default.
- W2893835395 cites W2026727321 @default.
- W2893835395 cites W2027663702 @default.
- W2893835395 cites W2027760729 @default.
- W2893835395 cites W2029790605 @default.
- W2893835395 cites W2030564316 @default.
- W2893835395 cites W2031292508 @default.
- W2893835395 cites W2034942377 @default.
- W2893835395 cites W2038286309 @default.
- W2893835395 cites W2039765580 @default.
- W2893835395 cites W2043846354 @default.
- W2893835395 cites W2045950270 @default.
- W2893835395 cites W2047705116 @default.
- W2893835395 cites W2049431608 @default.
- W2893835395 cites W2051882271 @default.
- W2893835395 cites W2053462801 @default.
- W2893835395 cites W2056446815 @default.
- W2893835395 cites W2057668415 @default.
- W2893835395 cites W2058394391 @default.
- W2893835395 cites W2060384627 @default.
- W2893835395 cites W2061172155 @default.
- W2893835395 cites W2063253418 @default.
- W2893835395 cites W2073745970 @default.
- W2893835395 cites W2073850595 @default.
- W2893835395 cites W2074263866 @default.
- W2893835395 cites W2074952395 @default.
- W2893835395 cites W2078933468 @default.
- W2893835395 cites W2082176639 @default.
- W2893835395 cites W2087774804 @default.
- W2893835395 cites W2091051573 @default.
- W2893835395 cites W2097026016 @default.
- W2893835395 cites W2104188396 @default.
- W2893835395 cites W2109939279 @default.
- W2893835395 cites W2109957799 @default.
- W2893835395 cites W2114185596 @default.
- W2893835395 cites W2115132771 @default.
- W2893835395 cites W2124047990 @default.
- W2893835395 cites W2124906886 @default.
- W2893835395 cites W2128246609 @default.
- W2893835395 cites W2128354083 @default.
- W2893835395 cites W2130323165 @default.
- W2893835395 cites W2134982128 @default.
- W2893835395 cites W2136956472 @default.
- W2893835395 cites W2138375082 @default.
- W2893835395 cites W2139420483 @default.
- W2893835395 cites W2140791749 @default.
- W2893835395 cites W2143300213 @default.
- W2893835395 cites W2150670276 @default.
- W2893835395 cites W2154080115 @default.
- W2893835395 cites W2157583738 @default.
- W2893835395 cites W2158490078 @default.