FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893838068> ?p ?o ?g. }

• W2893838068 endingPage "98" @default.
• W2893838068 startingPage "88" @default.
• W2893838068 abstract "Targeting the anti-apoptotic protein Mcl-1 holds a promise to improve therapy of multiple types of Mcl-1 dependent cancers but raises concerns of on-target cardiotoxicity due to the presence and reported role of Mcl-1 in heart. Herein, we investigated the importance of Mcl-1 in the survival and contractile function of human pluripotent stem cell-derived cardiomyocytes in culture. Effective knockdown of Mcl-1 with siRNAs reproducibly resulted in early (measured at Day 3) marginal alterations in caspase 3/7 activity, LDH leakage, ATP content and cellular impedance. After 14 days of Mcl-1 knockdown, loss of mitochondrial membrane potential, deteriorating effects on mitochondrial ultrastructure, and alterations in beat rate and amplitude were revealed. Inhibition of Bcl-xL by siRNA-mediated knockdown or selective inhibitors did not cause any overt cellular responses except for a minimal increase in caspase 3/7 activity; however, loss of Mcl-1 concomitant with down-regulated Bcl-xL activated apoptosis and caused extensive cell death as reflected by an 80% loss in cell index, activation of caspase-3 with associated PARP cleavage, and a decrease in beat amplitude and mitochondrial membrane potential after 3 days of Mcl-1/Bcl-xL knockdown., Together, these findings suggest that Mcl-1 and Bcl-xL provide duplicate safeguard measures in maintaining structural and functional integrity of cardiomyocytes. Hence, BH3-mimetic drugs targeting Mcl-1 may be well tolerated in the presence of intact Bcl-xL." @default.
• W2893838068 created "2018-10-05" @default.
• W2893838068 creator A5003200865 @default.
• W2893838068 creator A5015228968 @default.
• W2893838068 creator A5060855413 @default.
• W2893838068 creator A5079536020 @default.
• W2893838068 creator A5080876963 @default.
• W2893838068 date "2018-12-01" @default.
• W2893838068 modified "2023-09-27" @default.
• W2893838068 title "Role of Mcl-1 in regulation of cell death in human induced pluripotent stem cell-derived cardiomyocytes in vitro" @default.
• W2893838068 cites W1970124907 @default.
• W2893838068 cites W1979248048 @default.
• W2893838068 cites W1992954695 @default.
• W2893838068 cites W2048217074 @default.
• W2893838068 cites W2055194057 @default.
• W2893838068 cites W2070110113 @default.
• W2893838068 cites W2080059014 @default.
• W2893838068 cites W2088802752 @default.
• W2893838068 cites W2088883749 @default.
• W2893838068 cites W2096592089 @default.
• W2893838068 cites W2120308617 @default.
• W2893838068 cites W2136910701 @default.
• W2893838068 cites W2137240394 @default.
• W2893838068 cites W2139347878 @default.
• W2893838068 cites W2160650811 @default.
• W2893838068 cites W2167781187 @default.
• W2893838068 cites W2171224722 @default.
• W2893838068 cites W2534349779 @default.
• W2893838068 cites W2536818565 @default.
• W2893838068 cites W2755166564 @default.
• W2893838068 doi "https://doi.org/10.1016/j.taap.2018.09.041" @default.
• W2893838068 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30268579" @default.
• W2893838068 hasPublicationYear "2018" @default.
• W2893838068 type Work @default.
• W2893838068 sameAs 2893838068 @default.
• W2893838068 citedByCount "9" @default.
• W2893838068 countsByYear W28938380682019 @default.
• W2893838068 countsByYear W28938380682020 @default.
• W2893838068 countsByYear W28938380682021 @default.
• W2893838068 countsByYear W28938380682022 @default.
• W2893838068 countsByYear W28938380682023 @default.
• W2893838068 crossrefType "journal-article" @default.
• W2893838068 hasAuthorship W2893838068A5003200865 @default.
• W2893838068 hasAuthorship W2893838068A5015228968 @default.
• W2893838068 hasAuthorship W2893838068A5060855413 @default.
• W2893838068 hasAuthorship W2893838068A5079536020 @default.
• W2893838068 hasAuthorship W2893838068A5080876963 @default.
• W2893838068 hasConcept C104317684 @default.
• W2893838068 hasConcept C107459253 @default.
• W2893838068 hasConcept C12519072 @default.
• W2893838068 hasConcept C145103041 @default.
• W2893838068 hasConcept C173396325 @default.
• W2893838068 hasConcept C182979987 @default.
• W2893838068 hasConcept C190283241 @default.
• W2893838068 hasConcept C22615655 @default.
• W2893838068 hasConcept C2776789625 @default.
• W2893838068 hasConcept C28328180 @default.
• W2893838068 hasConcept C28859421 @default.
• W2893838068 hasConcept C31573885 @default.
• W2893838068 hasConcept C502942594 @default.
• W2893838068 hasConcept C54009773 @default.
• W2893838068 hasConcept C54355233 @default.
• W2893838068 hasConcept C55493867 @default.
• W2893838068 hasConcept C75385678 @default.
• W2893838068 hasConcept C81885089 @default.
• W2893838068 hasConcept C82381507 @default.
• W2893838068 hasConcept C86803240 @default.
• W2893838068 hasConcept C95444343 @default.
• W2893838068 hasConceptScore W2893838068C104317684 @default.
• W2893838068 hasConceptScore W2893838068C107459253 @default.
• W2893838068 hasConceptScore W2893838068C12519072 @default.
• W2893838068 hasConceptScore W2893838068C145103041 @default.
• W2893838068 hasConceptScore W2893838068C173396325 @default.
• W2893838068 hasConceptScore W2893838068C182979987 @default.
• W2893838068 hasConceptScore W2893838068C190283241 @default.
• W2893838068 hasConceptScore W2893838068C22615655 @default.
• W2893838068 hasConceptScore W2893838068C2776789625 @default.
• W2893838068 hasConceptScore W2893838068C28328180 @default.
• W2893838068 hasConceptScore W2893838068C28859421 @default.
• W2893838068 hasConceptScore W2893838068C31573885 @default.
• W2893838068 hasConceptScore W2893838068C502942594 @default.
• W2893838068 hasConceptScore W2893838068C54009773 @default.
• W2893838068 hasConceptScore W2893838068C54355233 @default.
• W2893838068 hasConceptScore W2893838068C55493867 @default.
• W2893838068 hasConceptScore W2893838068C75385678 @default.
• W2893838068 hasConceptScore W2893838068C81885089 @default.
• W2893838068 hasConceptScore W2893838068C82381507 @default.
• W2893838068 hasConceptScore W2893838068C86803240 @default.
• W2893838068 hasConceptScore W2893838068C95444343 @default.
• W2893838068 hasFunder F4320332161 @default.
• W2893838068 hasFunder F4320337351 @default.
• W2893838068 hasLocation W28938380681 @default.
• W2893838068 hasLocation W28938380682 @default.
• W2893838068 hasOpenAccess W2893838068 @default.
• W2893838068 hasPrimaryLocation W28938380681 @default.
• W2893838068 hasRelatedWork W1519566744 @default.
• W2893838068 hasRelatedWork W2019372185 @default.
• W2893838068 hasRelatedWork W2067565464 @default.

• next