FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893841223> ?p ?o ?g. }

• W2893841223 endingPage "17660" @default.
• W2893841223 startingPage "17646" @default.
• W2893841223 abstract "MicroRNAs (miRs) and Hox transcription factors have decisive roles in postnatal bone formation and homeostasis. In silico analysis identified extensive interaction between HOXA cluster mRNA and microRNAs from the miR-23a cluster. However, Hox regulation by the miR-23a cluster during osteoblast differentiation remains undefined. We examined this regulation in preosteoblasts and in a novel miR-23a cluster knockdown mouse model. Overexpression and knockdown of the miR-23a cluster in preosteoblasts decreased and increased, respectively, the expression of the proteins HOXA5, HOXA10, and HOXA11; these proteins’ mRNAs exhibited significant binding with the miR-23a cluster miRNAs, and miRNA 3′-UTR reporter assays confirmed repression. Importantly, during periods correlating with development and differentiation of bone cells, we found an inverse pattern of expression between HoxA factors and members of the miR-23a cluster. HOXA5 and HOXA11 bound to bone-specific promoters, physically interacted with transcription factor RUNX2, and regulated bone-specific genes. Depletion of HOXA5 or HOXA11 in preosteoblasts also decreased cellular differentiation. Additionally, stable overexpression of the miR-23a cluster in osteoblasts decreased the recruitment of HOXA5 and HOXA11 to osteoblast gene promoters, significantly inhibiting histone H3 acetylation. Heterozygous miR-23a cluster knockdown female mice (miR-23a ClWT/ZIP) had significantly increased trabecular bone mass when compared with WT mice. Furthermore, miR-23a cluster knockdown in calvarial osteoblasts of these mice increased the recruitment of HOXA5 and HOXA11, with a substantial enrichment of promoter histone H3 acetylation. Taken together, these findings demonstrate that the miR-23a cluster is required for maintaining stage-specific HoxA factor expression during osteogenesis. MicroRNAs (miRs) and Hox transcription factors have decisive roles in postnatal bone formation and homeostasis. In silico analysis identified extensive interaction between HOXA cluster mRNA and microRNAs from the miR-23a cluster. However, Hox regulation by the miR-23a cluster during osteoblast differentiation remains undefined. We examined this regulation in preosteoblasts and in a novel miR-23a cluster knockdown mouse model. Overexpression and knockdown of the miR-23a cluster in preosteoblasts decreased and increased, respectively, the expression of the proteins HOXA5, HOXA10, and HOXA11; these proteins’ mRNAs exhibited significant binding with the miR-23a cluster miRNAs, and miRNA 3′-UTR reporter assays confirmed repression. Importantly, during periods correlating with development and differentiation of bone cells, we found an inverse pattern of expression between HoxA factors and members of the miR-23a cluster. HOXA5 and HOXA11 bound to bone-specific promoters, physically interacted with transcription factor RUNX2, and regulated bone-specific genes. Depletion of HOXA5 or HOXA11 in preosteoblasts also decreased cellular differentiation. Additionally, stable overexpression of the miR-23a cluster in osteoblasts decreased the recruitment of HOXA5 and HOXA11 to osteoblast gene promoters, significantly inhibiting histone H3 acetylation. Heterozygous miR-23a cluster knockdown female mice (miR-23a ClWT/ZIP) had significantly increased trabecular bone mass when compared with WT mice. Furthermore, miR-23a cluster knockdown in calvarial osteoblasts of these mice increased the recruitment of HOXA5 and HOXA11, with a substantial enrichment of promoter histone H3 acetylation. Taken together, these findings demonstrate that the miR-23a cluster is required for maintaining stage-specific HoxA factor expression during osteogenesis." @default.
• W2893841223 created "2018-10-05" @default.
• W2893841223 creator A5003098266 @default.
• W2893841223 creator A5040238092 @default.
• W2893841223 creator A5049267438 @default.
• W2893841223 creator A5052785230 @default.
• W2893841223 creator A5053931778 @default.
• W2893841223 creator A5054432013 @default.
• W2893841223 creator A5063494061 @default.
• W2893841223 creator A5073326828 @default.
• W2893841223 creator A5079286294 @default.
• W2893841223 creator A5084167943 @default.
• W2893841223 creator A5086522266 @default.
• W2893841223 date "2018-11-01" @default.
• W2893841223 modified "2023-10-15" @default.
• W2893841223 title "The microRNA-23a cluster regulates the developmental HoxA cluster function during osteoblast differentiation" @default.
• W2893841223 cites W1509760969 @default.
• W2893841223 cites W1534759809 @default.
• W2893841223 cites W1768274470 @default.
• W2893841223 cites W1868418021 @default.
• W2893841223 cites W1879322767 @default.
• W2893841223 cites W1949769016 @default.
• W2893841223 cites W1966725512 @default.
• W2893841223 cites W1968414953 @default.
• W2893841223 cites W1968737620 @default.
• W2893841223 cites W1972879541 @default.
• W2893841223 cites W1974595624 @default.
• W2893841223 cites W1975072114 @default.
• W2893841223 cites W1975998449 @default.
• W2893841223 cites W1976612131 @default.
• W2893841223 cites W1977455818 @default.
• W2893841223 cites W1984762468 @default.
• W2893841223 cites W1985547648 @default.
• W2893841223 cites W1986623296 @default.
• W2893841223 cites W1986938057 @default.
• W2893841223 cites W1991090476 @default.
• W2893841223 cites W1999210109 @default.
• W2893841223 cites W1999382756 @default.
• W2893841223 cites W1999942858 @default.
• W2893841223 cites W2006055704 @default.
• W2893841223 cites W2009046969 @default.
• W2893841223 cites W2012330620 @default.
• W2893841223 cites W2016547417 @default.
• W2893841223 cites W2017435031 @default.
• W2893841223 cites W2019623019 @default.
• W2893841223 cites W2022689175 @default.
• W2893841223 cites W2026570758 @default.
• W2893841223 cites W2027318416 @default.
• W2893841223 cites W2028928108 @default.
• W2893841223 cites W2028990252 @default.
• W2893841223 cites W2030564922 @default.
• W2893841223 cites W2034803929 @default.
• W2893841223 cites W2037417726 @default.
• W2893841223 cites W2039191702 @default.
• W2893841223 cites W2042673698 @default.
• W2893841223 cites W2048249977 @default.
• W2893841223 cites W2049469517 @default.
• W2893841223 cites W2052790535 @default.
• W2893841223 cites W2058859268 @default.
• W2893841223 cites W2072269334 @default.
• W2893841223 cites W2074216622 @default.
• W2893841223 cites W2081134904 @default.
• W2893841223 cites W2087472570 @default.
• W2893841223 cites W2089125941 @default.
• W2893841223 cites W2093281361 @default.
• W2893841223 cites W2112255331 @default.
• W2893841223 cites W2118390503 @default.
• W2893841223 cites W2119962312 @default.
• W2893841223 cites W2120307929 @default.
• W2893841223 cites W2134078674 @default.
• W2893841223 cites W2134629862 @default.
• W2893841223 cites W2135271831 @default.
• W2893841223 cites W2154274801 @default.
• W2893841223 cites W2155756637 @default.
• W2893841223 cites W2156186506 @default.
• W2893841223 cites W2166972310 @default.
• W2893841223 cites W2170185022 @default.
• W2893841223 cites W2171166306 @default.
• W2893841223 cites W2216105743 @default.
• W2893841223 cites W2232740985 @default.
• W2893841223 cites W2474601281 @default.
• W2893841223 cites W2567004306 @default.
• W2893841223 cites W2606397279 @default.
• W2893841223 cites W2610445061 @default.
• W2893841223 cites W2610851752 @default.
• W2893841223 cites W609404933 @default.
• W2893841223 doi "https://doi.org/10.1074/jbc.ra118.003052" @default.
• W2893841223 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6231122" @default.
• W2893841223 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30242124" @default.
• W2893841223 hasPublicationYear "2018" @default.
• W2893841223 type Work @default.
• W2893841223 sameAs 2893841223 @default.
• W2893841223 citedByCount "31" @default.
• W2893841223 countsByYear W28938412232018 @default.
• W2893841223 countsByYear W28938412232019 @default.
• W2893841223 countsByYear W28938412232020 @default.
• W2893841223 countsByYear W28938412232021 @default.
• W2893841223 countsByYear W28938412232022 @default.

• next