FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893880518> ?p ?o ?g. }

• W2893880518 endingPage "1112" @default.
• W2893880518 startingPage "1100" @default.
• W2893880518 abstract "Abstract OBJECTIVE To evaluate effects of various concentrations of collagenase and dimethyl sulfoxide (DMSO) on yield of equine adipose-derived multipotent stromal cells (ASCs) before and after cryopreservation. SAMPLE Supragluteal subcutaneous adipose tissue from 7 Thoroughbreds. PROCEDURES Tissues were incubated with digests containing 0.1%, 0.05%, or 0.025% type I collagenase. Part of each resulting stromal vascular fraction was cryopreserved in 80% fetal bovine serum (FBS), 10% DMSO, and 10% Dulbecco modified Eagle medium F-12 and in 95% FBS and 5% DMSO. Half of each fresh and cryopreserved heterogeneous cell population was not immunophenotyped (unsorted) or was immunophenotyped for CD44 + , CD105 + , and major histocompatability complex class II (MHCII; CD44 + -CD105 + -MHCII + cells and CD44 + -CD105 + -MHCII − cells). Cell proliferation (cell viability assay), plasticity (CFU frequency), and lineage-specific target gene and oncogene expression (reverse transcriptase PCR assays) were determined in passage 1 cells before and after culture in induction media. RESULTS Digestion with 0.1% collagenase yielded the highest number of nucleated cells. Cell surface marker expression and proliferation rate were not affected by collagenase concentration. Cryopreservation reduced cell expansion rate and CD44 + -CD105 + -MHCII − CFUs; it also reduced osteogenic plasticity of unsorted cells. However, effects appeared to be unrelated to DMSO concentrations. There were also variable effects on primordial gene expression among cell isolates. CONCLUSIONS AND CLINICAL RELEVANCE Results supported the use of 0.1% collagenase in an adipose tissue digest and 5% DMSO in cryopreservation medium for isolation and cryopreservation, respectively, of equine ASCs. These results may be used as guidelines for standardization of isolation and cryopreservation procedures for equine ASCs." @default.
• W2893880518 created "2018-10-05" @default.
• W2893880518 creator A5030271967 @default.
• W2893880518 creator A5088285841 @default.
• W2893880518 date "2018-10-01" @default.
• W2893880518 modified "2023-10-16" @default.
• W2893880518 title "Effects of enzyme and cryoprotectant concentrations on yield of equine adipose-derived multipotent stromal cells" @default.
• W2893880518 cites W1478378344 @default.
• W2893880518 cites W1496192093 @default.
• W2893880518 cites W1497462806 @default.
• W2893880518 cites W1748891225 @default.
• W2893880518 cites W1947261266 @default.
• W2893880518 cites W1973268693 @default.
• W2893880518 cites W1976906682 @default.
• W2893880518 cites W1977865779 @default.
• W2893880518 cites W1978029855 @default.
• W2893880518 cites W1981359573 @default.
• W2893880518 cites W1981649139 @default.
• W2893880518 cites W1998566647 @default.
• W2893880518 cites W2000547590 @default.
• W2893880518 cites W2001705654 @default.
• W2893880518 cites W2009551466 @default.
• W2893880518 cites W2010608530 @default.
• W2893880518 cites W2021231315 @default.
• W2893880518 cites W2032560191 @default.
• W2893880518 cites W2035750370 @default.
• W2893880518 cites W2036491313 @default.
• W2893880518 cites W2039533211 @default.
• W2893880518 cites W2040511639 @default.
• W2893880518 cites W2047539360 @default.
• W2893880518 cites W2048342038 @default.
• W2893880518 cites W2049745633 @default.
• W2893880518 cites W2051051060 @default.
• W2893880518 cites W2056992430 @default.
• W2893880518 cites W2058033615 @default.
• W2893880518 cites W2060341044 @default.
• W2893880518 cites W2075876775 @default.
• W2893880518 cites W2081395034 @default.
• W2893880518 cites W2086015762 @default.
• W2893880518 cites W2089609786 @default.
• W2893880518 cites W2102085111 @default.
• W2893880518 cites W2114099446 @default.
• W2893880518 cites W2114434551 @default.
• W2893880518 cites W2115552126 @default.
• W2893880518 cites W2125582326 @default.
• W2893880518 cites W2135647805 @default.
• W2893880518 cites W2144796426 @default.
• W2893880518 cites W2147673404 @default.
• W2893880518 cites W2152752097 @default.
• W2893880518 cites W2154219172 @default.
• W2893880518 cites W2155012110 @default.
• W2893880518 cites W2167992899 @default.
• W2893880518 cites W2172234231 @default.
• W2893880518 cites W2173497140 @default.
• W2893880518 cites W2321103757 @default.
• W2893880518 cites W2593447272 @default.
• W2893880518 cites W2752813764 @default.
• W2893880518 cites W2762731973 @default.
• W2893880518 doi "https://doi.org/10.2460/ajvr.79.10.1100" @default.
• W2893880518 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30256145" @default.
• W2893880518 hasPublicationYear "2018" @default.
• W2893880518 type Work @default.
• W2893880518 sameAs 2893880518 @default.
• W2893880518 citedByCount "3" @default.
• W2893880518 countsByYear W28938805182019 @default.
• W2893880518 countsByYear W28938805182020 @default.
• W2893880518 countsByYear W28938805182022 @default.
• W2893880518 crossrefType "journal-article" @default.
• W2893880518 hasAuthorship W2893880518A5030271967 @default.
• W2893880518 hasAuthorship W2893880518A5088285841 @default.
• W2893880518 hasConcept C1491633281 @default.
• W2893880518 hasConcept C153911025 @default.
• W2893880518 hasConcept C16685009 @default.
• W2893880518 hasConcept C16930146 @default.
• W2893880518 hasConcept C171089720 @default.
• W2893880518 hasConcept C179933525 @default.
• W2893880518 hasConcept C181199279 @default.
• W2893880518 hasConcept C185592680 @default.
• W2893880518 hasConcept C196843134 @default.
• W2893880518 hasConcept C198826908 @default.
• W2893880518 hasConcept C203014093 @default.
• W2893880518 hasConcept C2778394429 @default.
• W2893880518 hasConcept C2779404806 @default.
• W2893880518 hasConcept C2780745355 @default.
• W2893880518 hasConcept C2780932548 @default.
• W2893880518 hasConcept C2908647359 @default.
• W2893880518 hasConcept C502942594 @default.
• W2893880518 hasConcept C55493867 @default.
• W2893880518 hasConcept C71924100 @default.
• W2893880518 hasConcept C86803240 @default.
• W2893880518 hasConcept C95444343 @default.
• W2893880518 hasConcept C99454951 @default.
• W2893880518 hasConceptScore W2893880518C1491633281 @default.
• W2893880518 hasConceptScore W2893880518C153911025 @default.
• W2893880518 hasConceptScore W2893880518C16685009 @default.
• W2893880518 hasConceptScore W2893880518C16930146 @default.
• W2893880518 hasConceptScore W2893880518C171089720 @default.
• W2893880518 hasConceptScore W2893880518C179933525 @default.

• next