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- W2893896552 abstract "Urea is the most important precursor of ethyl carbamate (EC), a harmful carcinogenic product, in fermented wines. In this study, the effects of four GATA transcriptional factors (Gln3p, Gat1p, Dal80p ,and Gzf3p) on extracellular urea and EC formation and transcriptional changes in urea degradation related genes (DUR1,2 and DUR3) were examined. Compared to the WT strain, the Δgzf3 mutant showed 18.7% urea reduction and exhibited synergistic effects with overexpressed Gln3p1–653 and Gat1p1–375 on extracellular urea reduction. Moreover, Δgzf3+Gln3p1–653 and Δgzf3+Gat1p1–375 showed significant 38.7% and 43.7% decreases in urea concentration and 41.7% and 48.5% decreases in EC concentration, respectively, in a model rice wine system. These results provide a promising way to reduce urea and EC formation during wine fermentation and raise some cues for the regulations of the four GATA transcriptional factors on the expression of individual nitrogen catabolite repression sensitive genes and their related metabolism pathway." @default.
- W2893896552 created "2018-10-05" @default.
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- W2893896552 date "2018-09-24" @default.
- W2893896552 modified "2023-10-10" @default.
- W2893896552 title "Metabolic Engineering of Four GATA Factors to Reduce Urea and Ethyl Carbamate Formation in a Model Rice Wine System" @default.
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- W2893896552 doi "https://doi.org/10.1021/acs.jafc.8b04370" @default.
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