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• W2893896755 abstract "B cells in fish are proven to be endocytic and have a great contribution to innate immunity like phagocytosis. In this study, the endocytic capacity and the corresponding internalization pathways of IgM+ B cells in Japanese flounder (Paralichthys olivaceus) were investigated. The results showed that IgM+ B cells in peripheral blood leukocytes (PBL) and splenic leukocytes (SL) exhibited different abilities to ingest 0.5 μm and 1 μm latex beads through macropinocytosis-dependent endocytic pathway. Japanese flounder CD22 (PoCD22) co-stimulatory signals were identified to be essential for the innate immune responses in B cells. Most of IgM+ B cells and some IgM− cells were demonstrated to be PoCD22 positive. When PoCD22 was blocked by antibody, the endocytic activities and reactive oxygen species (ROS) activities of SL IgM+ B cells were significantly increased, while the endocytic and ROS activities of PBL IgM+ B cells were significant decreased. These results collectively suggest that Japanese flounder IgM+ B cells are able to employ macropinocytosis-dependent endocytic pathway, which is under the regulation of CD22." @default.
• W2893896755 created "2018-10-05" @default.
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• W2893896755 date "2019-01-01" @default.
• W2893896755 modified "2023-10-14" @default.
• W2893896755 title "Macropinocytosis-dependent endocytosis of Japanese flounder IgM+ B cells and its regulation by CD22" @default.
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• W2893896755 doi "https://doi.org/10.1016/j.fsi.2018.09.068" @default.
• W2893896755 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30266605" @default.
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