Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893903477> ?p ?o ?g. }
- W2893903477 endingPage "965" @default.
- W2893903477 startingPage "957" @default.
- W2893903477 abstract "In this study, it was aimed to investigate the resistance rates of gentamicin, penicillin, methicillin, vancomycin, linezolid and tetracycline by phenotypic and genotypic methods in Staphylococcus aureus isolates and to determine plasmid content. Between the months of January and September in 2015, 100 clinical isolates of S. aureus were obtained from different samples such as wound, blood, urine. The automated bacteria identification and antibiotic susceptibility system (BD PhoenixTM, Sparks, MD, USA) was used to determine of antibiotic sensitivities. The resistance to methicillin was also investigated by Kirby-Bauer disc diffusion method using a 30 μg cefoxitin disc. The presence of aac(6’)/aph(2’’), blaZ, mecA, femA, vanA, vanB, cfr, tetK and tetM genes related to antibiotic resistance was investigated by PCR amplification in all isolates. Plasmid DNAs were isolated by using a Thermo Scientific GeneJET Plasmid Miniprep Kit. The cefoxitin resistance of S.aureus isolates, identified according to the results of disk diffusion and automated system, was calculated as 19%. Vancomycin and linezolid resistance were not observed in isolates while gentamicin 2%, penicillin 100%, methicillin 19%, tetracycline 18% resistance were identified using the automated system. According to the results of molecular analysis aac(6’)/aph(2’’), blaZ, mecA, femA, tetK and tetM genes frequencies were determined as 2%, 100%, 19%, 100%, 17% and 3% respectively, but vanA, vanB and cfr genes were not amplified by PCR. In order to determine the relationship between antibiotic resistance and plasmid presence, plasmids were isolated from identified bacterial isolates. It is found that most of bacterial isolates (79%) contain different numbers plasmids. Rapid and reliable method for antibiotic susceptibility is important to determine the appropriate therapy decision. PCR can be used for confirmation of the results obtained by automated system or could be used as an alternative diagnostic method in the routine diagnosis for rapid, sensitive, and specific detection of MRSA associated antibiotic resistance genes." @default.
- W2893903477 created "2018-10-05" @default.
- W2893903477 creator A5047763614 @default.
- W2893903477 creator A5052247566 @default.
- W2893903477 creator A5064502600 @default.
- W2893903477 creator A5070726636 @default.
- W2893903477 date "2018-11-30" @default.
- W2893903477 modified "2023-10-18" @default.
- W2893903477 title "Phenotypic and Genotypic Analysis of Gentamicin, Penicillin, Methicillin, Vancomycin, Linezolid and Tetracycline Resistance in Clinical Isolates of Staphylococcus aureus" @default.
- W2893903477 cites W1925363657 @default.
- W2893903477 cites W1968360092 @default.
- W2893903477 cites W1983784984 @default.
- W2893903477 cites W2000415512 @default.
- W2893903477 cites W2019041420 @default.
- W2893903477 cites W2021599157 @default.
- W2893903477 cites W2027621451 @default.
- W2893903477 cites W2034546252 @default.
- W2893903477 cites W2034629372 @default.
- W2893903477 cites W2047363759 @default.
- W2893903477 cites W2059901223 @default.
- W2893903477 cites W2062599976 @default.
- W2893903477 cites W2067168800 @default.
- W2893903477 cites W2088371041 @default.
- W2893903477 cites W2091404988 @default.
- W2893903477 cites W2102419473 @default.
- W2893903477 cites W2104074973 @default.
- W2893903477 cites W2104683386 @default.
- W2893903477 cites W2106861841 @default.
- W2893903477 cites W2109402884 @default.
- W2893903477 cites W2110348594 @default.
- W2893903477 cites W2111662072 @default.
- W2893903477 cites W2111691760 @default.
- W2893903477 cites W2117605828 @default.
- W2893903477 cites W2118676042 @default.
- W2893903477 cites W2118707751 @default.
- W2893903477 cites W2125066910 @default.
- W2893903477 cites W2125464975 @default.
- W2893903477 cites W2128213617 @default.
- W2893903477 cites W2134328505 @default.
- W2893903477 cites W2137152929 @default.
- W2893903477 cites W2142705881 @default.
- W2893903477 cites W2144234849 @default.
- W2893903477 cites W2147122404 @default.
- W2893903477 cites W2149234599 @default.
- W2893903477 cites W2153182750 @default.
- W2893903477 cites W2158830887 @default.
- W2893903477 cites W2169704607 @default.
- W2893903477 cites W2170786573 @default.
- W2893903477 cites W2590781933 @default.
- W2893903477 cites W2605699627 @default.
- W2893903477 cites W2782489043 @default.
- W2893903477 cites W2791244272 @default.
- W2893903477 cites W2791609775 @default.
- W2893903477 doi "https://doi.org/10.18016/ksutarimdoga.vi.452783" @default.
- W2893903477 hasPublicationYear "2018" @default.
- W2893903477 type Work @default.
- W2893903477 sameAs 2893903477 @default.
- W2893903477 citedByCount "0" @default.
- W2893903477 crossrefType "journal-article" @default.
- W2893903477 hasAuthorship W2893903477A5047763614 @default.
- W2893903477 hasAuthorship W2893903477A5052247566 @default.
- W2893903477 hasAuthorship W2893903477A5064502600 @default.
- W2893903477 hasAuthorship W2893903477A5070726636 @default.
- W2893903477 hasBestOaLocation W28939034771 @default.
- W2893903477 hasConcept C104317684 @default.
- W2893903477 hasConcept C174802600 @default.
- W2893903477 hasConcept C22744801 @default.
- W2893903477 hasConcept C2775832221 @default.
- W2893903477 hasConcept C2776124114 @default.
- W2893903477 hasConcept C2777052132 @default.
- W2893903477 hasConcept C2778980435 @default.
- W2893903477 hasConcept C2779489039 @default.
- W2893903477 hasConcept C2780923616 @default.
- W2893903477 hasConcept C2781414143 @default.
- W2893903477 hasConcept C501593827 @default.
- W2893903477 hasConcept C523546767 @default.
- W2893903477 hasConcept C54355233 @default.
- W2893903477 hasConcept C86803240 @default.
- W2893903477 hasConcept C89423630 @default.
- W2893903477 hasConcept C94665300 @default.
- W2893903477 hasConceptScore W2893903477C104317684 @default.
- W2893903477 hasConceptScore W2893903477C174802600 @default.
- W2893903477 hasConceptScore W2893903477C22744801 @default.
- W2893903477 hasConceptScore W2893903477C2775832221 @default.
- W2893903477 hasConceptScore W2893903477C2776124114 @default.
- W2893903477 hasConceptScore W2893903477C2777052132 @default.
- W2893903477 hasConceptScore W2893903477C2778980435 @default.
- W2893903477 hasConceptScore W2893903477C2779489039 @default.
- W2893903477 hasConceptScore W2893903477C2780923616 @default.
- W2893903477 hasConceptScore W2893903477C2781414143 @default.
- W2893903477 hasConceptScore W2893903477C501593827 @default.
- W2893903477 hasConceptScore W2893903477C523546767 @default.
- W2893903477 hasConceptScore W2893903477C54355233 @default.
- W2893903477 hasConceptScore W2893903477C86803240 @default.
- W2893903477 hasConceptScore W2893903477C89423630 @default.
- W2893903477 hasConceptScore W2893903477C94665300 @default.
- W2893903477 hasIssue "6" @default.
- W2893903477 hasLocation W28939034771 @default.