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- W2893962045 abstract "We studied the structure, function and thermodynamic properties for the unfolding of the Triosephosphate isomerase (TIM) from Zea mays (ZmTIM). ZmTIM shows a catalytic efficiency close to the diffusion limit. Native ZmTIM is a dimer that dissociates upon dilution into inactive and unfolded monomers. Its thermal unfolding is irreversible with a Tm of 61.6 ± 1.4 °C and an activation energy of 383.4 ± 11.5 kJ mol−1. The urea-induced unfolding of ZmTIM is reversible. Transitions followed by catalytic activity and spectroscopic properties are monophasic and superimposable, indicating that ZmTIM unfolds/refolds in a two-state behavior with an unfolding ΔG°(H20) = 99.8 ± 5.3 kJ mol−1. This contrasts with most other studied TIMs, where folding intermediates are common. The three-dimensional structure of ZmTIM was solved at 1.8 Å. A structural comparison with other eukaryotic TIMs shows a similar number of intramolecular and intermolecular interactions. Interestingly the number of interfacial water molecules found in ZmTIM is lower than those observed in most TIMs that show folding intermediates. Although with the available data, there is no clear correlation between structural properties and the number of equilibrium intermediates in the unfolding of TIM, the identification of such structural properties should increase our understanding of folding mechanisms." @default.
- W2893962045 created "2018-10-05" @default.
- W2893962045 creator A5008853600 @default.
- W2893962045 creator A5021796088 @default.
- W2893962045 creator A5023164009 @default.
- W2893962045 creator A5062338185 @default.
- W2893962045 creator A5090818077 @default.
- W2893962045 date "2018-11-01" @default.
- W2893962045 modified "2023-10-17" @default.
- W2893962045 title "Structure and conformational stability of the triosephosphate isomerase from Zea mays. Comparison with the chemical unfolding pathways of other eukaryotic TIMs" @default.
- W2893962045 cites W1494859323 @default.
- W2893962045 cites W1500290338 @default.
- W2893962045 cites W1505216348 @default.
- W2893962045 cites W1505900198 @default.
- W2893962045 cites W1526967281 @default.
- W2893962045 cites W1888176084 @default.
- W2893962045 cites W1926915036 @default.
- W2893962045 cites W194987105 @default.
- W2893962045 cites W1965151258 @default.
- W2893962045 cites W1966105237 @default.
- W2893962045 cites W1966946837 @default.
- W2893962045 cites W1972461753 @default.
- W2893962045 cites W1973706355 @default.
- W2893962045 cites W1974579447 @default.
- W2893962045 cites W1974913827 @default.
- W2893962045 cites W1974970687 @default.
- W2893962045 cites W1975460133 @default.
- W2893962045 cites W1975653215 @default.
- W2893962045 cites W1976762077 @default.
- W2893962045 cites W1986898368 @default.
- W2893962045 cites W1988671180 @default.
- W2893962045 cites W1989904058 @default.
- W2893962045 cites W1990428289 @default.
- W2893962045 cites W1991890586 @default.
- W2893962045 cites W1994267130 @default.
- W2893962045 cites W1997703477 @default.
- W2893962045 cites W1998140022 @default.
- W2893962045 cites W1998355071 @default.
- W2893962045 cites W1998858758 @default.
- W2893962045 cites W2009610308 @default.
- W2893962045 cites W2013509150 @default.
- W2893962045 cites W2014162883 @default.
- W2893962045 cites W2022987595 @default.
- W2893962045 cites W2023633717 @default.
- W2893962045 cites W2025854072 @default.
- W2893962045 cites W2025938842 @default.
- W2893962045 cites W2026188828 @default.
- W2893962045 cites W2026333941 @default.
- W2893962045 cites W2030061336 @default.
- W2893962045 cites W2031545478 @default.
- W2893962045 cites W2035467972 @default.
- W2893962045 cites W2035503835 @default.
- W2893962045 cites W2039215531 @default.
- W2893962045 cites W2039423289 @default.
- W2893962045 cites W2043925446 @default.
- W2893962045 cites W2044672456 @default.
- W2893962045 cites W2048087151 @default.
- W2893962045 cites W2051628563 @default.
- W2893962045 cites W2054932982 @default.
- W2893962045 cites W2055022749 @default.
- W2893962045 cites W2056618405 @default.
- W2893962045 cites W2064288341 @default.
- W2893962045 cites W2064589158 @default.
- W2893962045 cites W2068353165 @default.
- W2893962045 cites W2069309953 @default.
- W2893962045 cites W2073086002 @default.
- W2893962045 cites W2075215946 @default.
- W2893962045 cites W2082672971 @default.
- W2893962045 cites W2082846543 @default.
- W2893962045 cites W2084091052 @default.
- W2893962045 cites W2084418790 @default.
- W2893962045 cites W2085232944 @default.
- W2893962045 cites W2088288669 @default.
- W2893962045 cites W2089827651 @default.
- W2893962045 cites W2101168968 @default.
- W2893962045 cites W2102033493 @default.
- W2893962045 cites W2103098023 @default.
- W2893962045 cites W2104925184 @default.
- W2893962045 cites W2106315897 @default.
- W2893962045 cites W2107715579 @default.
- W2893962045 cites W2108921801 @default.
- W2893962045 cites W2109279623 @default.
- W2893962045 cites W2119588189 @default.
- W2893962045 cites W2121704271 @default.
- W2893962045 cites W2122134175 @default.
- W2893962045 cites W2122339645 @default.
- W2893962045 cites W2125864238 @default.
- W2893962045 cites W2130479394 @default.
- W2893962045 cites W2134623266 @default.
- W2893962045 cites W2135945776 @default.
- W2893962045 cites W2136789143 @default.
- W2893962045 cites W2140768489 @default.
- W2893962045 cites W2141131756 @default.
- W2893962045 cites W2144081223 @default.
- W2893962045 cites W2144369707 @default.
- W2893962045 cites W2144513255 @default.
- W2893962045 cites W2155242779 @default.
- W2893962045 cites W2160378127 @default.