FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893971796> ?p ?o ?g. }

• W2893971796 endingPage "213" @default.
• W2893971796 startingPage "205" @default.
• W2893971796 abstract "The interaction of Escherichia coli DNA-dependent RNA polymerase (EC 2.7.7.6) with the replicative form of the DNA from the filamentous coliphage fd cleaved by the restriction endonuclease HindII has been studied by electron microscopy at low and high ionic strength. In the presence of ATP or GTP, and heparin, RNA polymerase binds to fd replicative-form DNA at a few specific sites which have been mapped. The map was oriented so that transcription is from right to left. Three main GTP initiator sites are found at 15%, 82% and 94% of the genome length. One main ATP initiator site is found which cannot be mapped with the same accuracy, and which is localized between 38% and 50%. In the absence of initiator triphosphates and heparin, the binding of the enzyme to fd DNA is much more heterogeneous and therefore the mapping is more difficult. Nevertheless it seems that the preferential binding regions correspond to the specific sites mapped in the presence of GTP or ATP. The mean number of polymerase molecules bound to DNA as a function of the molecular ratio enzyme to DNA present in the mixture has been determined. From these results a binding isotherm can be obtained. The apparent equilibrium constant (K∼ 109 M−1) which is derived certainly represents an underestimated value, as discussed." @default.
• W2893971796 created "2018-10-05" @default.
• W2893971796 creator A5009481799 @default.
• W2893971796 creator A5062790722 @default.
• W2893971796 creator A5084555639 @default.
• W2893971796 date "1977-08-01" @default.
• W2893971796 modified "2023-09-23" @default.
• W2893971796 title "Electron Microscopy Analysis of the Interaction between Escherichia coli DNA-Dependent RNA Polymerase and the Replicative Form of Phage fd DNA. 1. Mapping of the Binding Sites" @default.
• W2893971796 cites W1486538157 @default.
• W2893971796 cites W1538472156 @default.
• W2893971796 cites W1540661436 @default.
• W2893971796 cites W1550973861 @default.
• W2893971796 cites W1569045429 @default.
• W2893971796 cites W1775749144 @default.
• W2893971796 cites W1866284050 @default.
• W2893971796 cites W1970072335 @default.
• W2893971796 cites W1971315567 @default.
• W2893971796 cites W1973108797 @default.
• W2893971796 cites W1975975928 @default.
• W2893971796 cites W1977623218 @default.
• W2893971796 cites W1984938906 @default.
• W2893971796 cites W1995192799 @default.
• W2893971796 cites W2000112820 @default.
• W2893971796 cites W2004455399 @default.
• W2893971796 cites W2004837297 @default.
• W2893971796 cites W2004904794 @default.
• W2893971796 cites W2008566545 @default.
• W2893971796 cites W2014731564 @default.
• W2893971796 cites W2024391369 @default.
• W2893971796 cites W2029963910 @default.
• W2893971796 cites W2047722476 @default.
• W2893971796 cites W2053992401 @default.
• W2893971796 cites W2063937509 @default.
• W2893971796 cites W2067660191 @default.
• W2893971796 cites W2067796661 @default.
• W2893971796 cites W2098132471 @default.
• W2893971796 cites W2132481027 @default.
• W2893971796 cites W2147082747 @default.
• W2893971796 cites W2161869756 @default.
• W2893971796 doi "https://doi.org/10.1111/j.1432-1033.1977.tb11731.x" @default.
• W2893971796 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/334531" @default.
• W2893971796 hasPublicationYear "1977" @default.
• W2893971796 type Work @default.
• W2893971796 sameAs 2893971796 @default.
• W2893971796 citedByCount "32" @default.
• W2893971796 crossrefType "journal-article" @default.
• W2893971796 hasAuthorship W2893971796A5009481799 @default.
• W2893971796 hasAuthorship W2893971796A5062790722 @default.
• W2893971796 hasAuthorship W2893971796A5084555639 @default.
• W2893971796 hasBestOaLocation W28939717961 @default.
• W2893971796 hasConcept C104317684 @default.
• W2893971796 hasConcept C107824862 @default.
• W2893971796 hasConcept C118716 @default.
• W2893971796 hasConcept C138885662 @default.
• W2893971796 hasConcept C153911025 @default.
• W2893971796 hasConcept C156719811 @default.
• W2893971796 hasConcept C164965036 @default.
• W2893971796 hasConcept C179926584 @default.
• W2893971796 hasConcept C181199279 @default.
• W2893971796 hasConcept C185581394 @default.
• W2893971796 hasConcept C185592680 @default.
• W2893971796 hasConcept C194830204 @default.
• W2893971796 hasConcept C2756471 @default.
• W2893971796 hasConcept C2776449523 @default.
• W2893971796 hasConcept C31254050 @default.
• W2893971796 hasConcept C41895202 @default.
• W2893971796 hasConcept C547475151 @default.
• W2893971796 hasConcept C552990157 @default.
• W2893971796 hasConcept C55493867 @default.
• W2893971796 hasConcept C67705224 @default.
• W2893971796 hasConcept C82381507 @default.
• W2893971796 hasConcept C86803240 @default.
• W2893971796 hasConcept C88354546 @default.
• W2893971796 hasConceptScore W2893971796C104317684 @default.
• W2893971796 hasConceptScore W2893971796C107824862 @default.
• W2893971796 hasConceptScore W2893971796C118716 @default.
• W2893971796 hasConceptScore W2893971796C138885662 @default.
• W2893971796 hasConceptScore W2893971796C153911025 @default.
• W2893971796 hasConceptScore W2893971796C156719811 @default.
• W2893971796 hasConceptScore W2893971796C164965036 @default.
• W2893971796 hasConceptScore W2893971796C179926584 @default.
• W2893971796 hasConceptScore W2893971796C181199279 @default.
• W2893971796 hasConceptScore W2893971796C185581394 @default.
• W2893971796 hasConceptScore W2893971796C185592680 @default.
• W2893971796 hasConceptScore W2893971796C194830204 @default.
• W2893971796 hasConceptScore W2893971796C2756471 @default.
• W2893971796 hasConceptScore W2893971796C2776449523 @default.
• W2893971796 hasConceptScore W2893971796C31254050 @default.
• W2893971796 hasConceptScore W2893971796C41895202 @default.
• W2893971796 hasConceptScore W2893971796C547475151 @default.
• W2893971796 hasConceptScore W2893971796C552990157 @default.
• W2893971796 hasConceptScore W2893971796C55493867 @default.
• W2893971796 hasConceptScore W2893971796C67705224 @default.
• W2893971796 hasConceptScore W2893971796C82381507 @default.
• W2893971796 hasConceptScore W2893971796C86803240 @default.
• W2893971796 hasConceptScore W2893971796C88354546 @default.
• W2893971796 hasIssue "1" @default.
• W2893971796 hasLocation W28939717961 @default.

• next