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- W2893995112 abstract "In Japan, Livestock Improvement Association of Japan started commercially producing sexed bovine semen 10 years ago, and sexed bovine semen is currently used for the artificial insemination (AI) in the farms. In this review, the authors introduce the technology for sperm sexing by flow cytometry, the efforts at commercializing sexed semen in Japan, and recent field data on artificial insemination of the cattle with sexed semen.In the procedures of the flow cytometric method, X-chromosome-bearing sperm and Y-chromosome-bearing sperm were fluorescently stained, separated from each other by analyzing the difference in the DNA content, and cryopreserved. The authors surveyed the conception rates after AI with these sperm and sex ratios of the offspring with the cooperation from livestock associations, AI technicians, and farmers.Although AI with sexed semen was associated with lower conception rates in comparison with AI with conventional semen, the accuracy of sex selection using AI with sexed semen was beyond >90%.Sexed semen produced by flow cytometry has the potential to produce offspring of the preferred sex with high accuracy and reliability. Thus, it is expected that sexed semen is used for AI more frequently in the farms." @default.
- W2893995112 created "2018-10-05" @default.
- W2893995112 creator A5023974568 @default.
- W2893995112 creator A5050615254 @default.
- W2893995112 creator A5056517079 @default.
- W2893995112 creator A5076630162 @default.
- W2893995112 date "2018-09-27" @default.
- W2893995112 modified "2023-09-26" @default.
- W2893995112 title "Bovine sperm sex-selection technology in Japan" @default.
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