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- W2894014039 startingPage "2997" @default.
- W2894014039 abstract "Astrocytes respond to neuronal activity by generating calcium signals which are implicated in the regulation of astroglial housekeeping functions and/or in modulation of synaptic transmission. We hypothesized that activity-induced calcium signals in astrocytes may activate calcineurin (CaN), a calcium/calmodulin-regulated protein phosphatase, implicated in neuropathology, but whose role in astroglial physiology remains unclear. We used a lentiviral vector expressing NFAT-EYFP (NY) fluorescent calcineurin sensor and a chemical protocol of LTP induction (cLTP) to show that, in mixed neuron-astrocytic hippocampal cultures, cLTP induced robust NY translocation into astrocyte nuclei and, hence, CaN activation. NY translocation was abolished by the CaN inhibitor FK506, and was not observed in pure astroglial cultures. Using Fura-2 single cell calcium imaging, we found sustained Ca2+ elevations in juxtaneuronal, but not distal, astrocytes. Pharmacological analysis revealed that both the Ca2+ signals and the nuclear NY translocation in astrocytes required NMDA and mGluR5 receptors and depended on extracellular Ca2+ entry via a store-operated mechanism. Our results provide a proof of principle that calcineurin in astrocytes may be activated in response to neuronal activity, thereby delineating a framework for investigating the role of astroglial CaN in the physiology of central nervous system." @default.
- W2894014039 created "2018-10-05" @default.
- W2894014039 creator A5024096995 @default.
- W2894014039 creator A5026120374 @default.
- W2894014039 creator A5043265559 @default.
- W2894014039 creator A5079962232 @default.
- W2894014039 creator A5091093273 @default.
- W2894014039 date "2018-09-30" @default.
- W2894014039 modified "2023-10-14" @default.
- W2894014039 title "Neuronal Activity-Dependent Activation of Astroglial Calcineurin in Mouse Primary Hippocampal Cultures" @default.
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- W2894014039 doi "https://doi.org/10.3390/ijms19102997" @default.
- W2894014039 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6213389" @default.
- W2894014039 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30274399" @default.
- W2894014039 hasPublicationYear "2018" @default.
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