FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894072337> ?p ?o ?g. }

• W2894072337 abstract "Abstract The adherens junction couples the actin cytoskeletons of neighboring cells to provide the foundation for multicellular organization. The core of the adherens junction is the cadherin-catenin complex that arose early in the evolution of multicellularity to link cortical actin to intercellular adhesions. Over time, evolutionary pressures have shaped the signaling and mechanical functions of the adherens junction to meet specific developmental and physiological demands. Evolutionary rate covariation (ERC) identifies genes with correlated fluctuations in evolutionary rate that can reflect shared selective pressures and functions. Here we use ERC to identify genes with evolutionary histories similar to shotgun (shg) , which encodes the Drosophila E-cadherin (DE-Cad) ortholog. Core adherens junction components α-catenin and p120-catenin displayed strong ERC correlations with shg, indicating that they evolved under similar selective pressures during evolution between Drosophila species. Further analysis of the shg ERC profile revealed a collection of genes not previously associated with shg function or cadherin-mediated adhesion. We then analyzed the function of a subset of ERC-identified candidate genes by RNAi during border cell (BC) migration and identified novel genes that function to regulate DE-Cad. Among these, we found that the gene CG42684 , which encodes a putative GTPase activating protein (GAP), regulates BC migration and adhesion. We named CG42684 raskol (“to split” in Russian) and show that it regulates DE-Cad levels and actin protrusions in BCs. We propose that Raskol functions with DE-Cad to restrict Ras/Rho signaling and help guide BC migration. Our results demonstrate that a coordinated selective pressure has shaped the adherens junction and this can be leveraged to identify novel components of the complexes and signaling pathways that regulate cadherin-mediated adhesion. Author Summary The establishment of intercellular adhesions facilitated the genesis of multicellular organisms. The adherens junction, which links the actin cytoskeletons of neighboring cells, arose early in the evolution of multicellularity and selective pressures have shaped its function and molecular composition over time. In this study, we used evolutionary rate covariation (ERC) analysis to examine the evolutionary history of the adherens junction and to identify genes that coevolved with the adherens junction gene shotgun, which encodes the Drosophila E-cadherin (DE-Cad). ERC analysis of shotgun revealed a collection of genes with similar evolutionary histories. We then tested the role of these genes in border cell migration in the fly egg chamber, a process that requires the coordinated regulation of cell-cell adhesion and cell motility. Among these, we found that a previously uncharacterized gene CG42684 , which encodes a putative GTPase activating protein (GAP), regulates the collective cell migration of border cells, stabilizes cell-cell adhesions and regulates the actin dynamics. Our results demonstrate that components of the adherens junction share an evolutionary history and that ERC analysis is a powerful method to identify novel components of cell adhesion complexes in Drosophila ." @default.
• W2894072337 created "2018-10-05" @default.
• W2894072337 creator A5002850706 @default.
• W2894072337 creator A5015859389 @default.
• W2894072337 creator A5034261660 @default.
• W2894072337 creator A5042515616 @default.
• W2894072337 creator A5064931312 @default.
• W2894072337 creator A5072066245 @default.
• W2894072337 creator A5075446824 @default.
• W2894072337 creator A5083973246 @default.
• W2894072337 date "2018-09-27" @default.
• W2894072337 modified "2023-09-27" @default.
• W2894072337 title "Evolutionary rate covariation analysis of E-cadherin identifies Raskol as regulator of cell adhesion and actin dynamics in<i>Drosophila</i>" @default.
• W2894072337 cites W12760659 @default.
• W2894072337 cites W1500746243 @default.
• W2894072337 cites W1605984840 @default.
• W2894072337 cites W1634818393 @default.
• W2894072337 cites W1841412170 @default.
• W2894072337 cites W1900937478 @default.
• W2894072337 cites W1930457684 @default.
• W2894072337 cites W1967803088 @default.
• W2894072337 cites W1968650783 @default.
• W2894072337 cites W1975611299 @default.
• W2894072337 cites W1975971309 @default.
• W2894072337 cites W1976314018 @default.
• W2894072337 cites W1979600479 @default.
• W2894072337 cites W1981566818 @default.
• W2894072337 cites W1983096415 @default.
• W2894072337 cites W1987938686 @default.
• W2894072337 cites W2006419043 @default.
• W2894072337 cites W2008297562 @default.
• W2894072337 cites W2013375714 @default.
• W2894072337 cites W2017777683 @default.
• W2894072337 cites W2019109537 @default.
• W2894072337 cites W2019398764 @default.
• W2894072337 cites W2020685225 @default.
• W2894072337 cites W2022090845 @default.
• W2894072337 cites W2027174548 @default.
• W2894072337 cites W2029071420 @default.
• W2894072337 cites W2039942560 @default.
• W2894072337 cites W2041677910 @default.
• W2894072337 cites W2047531031 @default.
• W2894072337 cites W2069202441 @default.
• W2894072337 cites W2069878364 @default.
• W2894072337 cites W2080870494 @default.
• W2894072337 cites W2085659118 @default.
• W2894072337 cites W2086972041 @default.
• W2894072337 cites W2093392157 @default.
• W2894072337 cites W2109472768 @default.
• W2894072337 cites W2109920103 @default.
• W2894072337 cites W2110335151 @default.
• W2894072337 cites W2111950378 @default.
• W2894072337 cites W2112731704 @default.
• W2894072337 cites W2121053101 @default.
• W2894072337 cites W2124593926 @default.
• W2894072337 cites W2141052558 @default.
• W2894072337 cites W2142742974 @default.
• W2894072337 cites W2145013849 @default.
• W2894072337 cites W2148860972 @default.
• W2894072337 cites W2156299846 @default.
• W2894072337 cites W2160300536 @default.
• W2894072337 cites W2161007406 @default.
• W2894072337 cites W2172290094 @default.
• W2894072337 cites W2174430314 @default.
• W2894072337 cites W2192249410 @default.
• W2894072337 cites W2195399807 @default.
• W2894072337 cites W2232779221 @default.
• W2894072337 cites W2258289096 @default.
• W2894072337 cites W2328192806 @default.
• W2894072337 cites W2556267806 @default.
• W2894072337 cites W2567824251 @default.
• W2894072337 cites W2593969344 @default.
• W2894072337 cites W2609731748 @default.
• W2894072337 cites W2790556376 @default.
• W2894072337 cites W2793152198 @default.
• W2894072337 cites W2807591256 @default.
• W2894072337 cites W40021010 @default.
• W2894072337 doi "https://doi.org/10.1101/429472" @default.
• W2894072337 hasPublicationYear "2018" @default.
• W2894072337 type Work @default.
• W2894072337 sameAs 2894072337 @default.
• W2894072337 citedByCount "0" @default.
• W2894072337 crossrefType "posted-content" @default.
• W2894072337 hasAuthorship W2894072337A5002850706 @default.
• W2894072337 hasAuthorship W2894072337A5015859389 @default.
• W2894072337 hasAuthorship W2894072337A5034261660 @default.
• W2894072337 hasAuthorship W2894072337A5042515616 @default.
• W2894072337 hasAuthorship W2894072337A5064931312 @default.
• W2894072337 hasAuthorship W2894072337A5072066245 @default.
• W2894072337 hasAuthorship W2894072337A5075446824 @default.
• W2894072337 hasAuthorship W2894072337A5083973246 @default.
• W2894072337 hasBestOaLocation W28940723371 @default.
• W2894072337 hasConcept C104317684 @default.
• W2894072337 hasConcept C125705527 @default.
• W2894072337 hasConcept C14036430 @default.
• W2894072337 hasConcept C1491633281 @default.
• W2894072337 hasConcept C149402561 @default.
• W2894072337 hasConcept C201508349 @default.
• W2894072337 hasConcept C54355233 @default.
• W2894072337 hasConcept C74318829 @default.

• next