FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894140384> ?p ?o ?g. }

• W2894140384 endingPage "187" @default.
• W2894140384 startingPage "178" @default.
• W2894140384 abstract "Loop-mediated isothermal amplification (LAMP) is a promising, simple, rapid and sensitive molecular detection method. In the present study, LAMP assay was developed for detecting Clostridium perfringens in chevon. Primers were designed to detect the cpa gene of C. perfringens. A panel of 19 bacterial strains, including 3 C. perfringens and 16 other strains, were included in this study to standardize and evaluate the LAMP assay. No false positive amplification was observed indicating 100% specificity of the assay. The detection limit of LAMP and conventional PCR in the DNA extracted from pure C. perfringens was 0.34 pg and 3.4 pg, respectively. This revealed that LAMP assay is 10 times more sensitive than conventional PCR. The sensitivity of the LAMP assay for the detection of C. perfringens in raw chevon was found to be 1.2 × 102 CFU/g after 6-h enrichment and 1.2 × 105 CFU/g without enrichment in artificial spiking studies. Improved C. perfringens detection of 12 CFU/g within 12 h was obtained proving that LAMP assay is significantly faster than traditional methods that take >2 d. The developed LAMP assay also detected the targeted organism in clinical and environmental samples with the sensitivity and specificity of 97% and 84%, respectively with Kappa agreement of 0.824 respects to PCR assay. This method shows immense potential for routine diagnosis and monitoring of C. perfringens in food, environment and clinical samples. This is the first report in which the LAMP assay was optimized for the detection of C. perfringens in chevon." @default.
• W2894140384 created "2018-10-05" @default.
• W2894140384 creator A5009643835 @default.
• W2894140384 creator A5048444823 @default.
• W2894140384 creator A5048999261 @default.
• W2894140384 creator A5051951753 @default.
• W2894140384 creator A5052733067 @default.
• W2894140384 creator A5058420371 @default.
• W2894140384 creator A5077327874 @default.
• W2894140384 creator A5078720732 @default.
• W2894140384 creator A5089135192 @default.
• W2894140384 date "2018-12-01" @default.
• W2894140384 modified "2023-09-23" @default.
• W2894140384 title "Development and evaluation of isothermal amplification assay for the rapid and sensitive detection of Clostridium perfringens from chevon" @default.
• W2894140384 cites W1575096162 @default.
• W2894140384 cites W1829008618 @default.
• W2894140384 cites W1968045298 @default.
• W2894140384 cites W1979974453 @default.
• W2894140384 cites W1988682094 @default.
• W2894140384 cites W1996465576 @default.
• W2894140384 cites W1999427888 @default.
• W2894140384 cites W2041848175 @default.
• W2894140384 cites W2044282964 @default.
• W2894140384 cites W2049878313 @default.
• W2894140384 cites W2057876450 @default.
• W2894140384 cites W2058007140 @default.
• W2894140384 cites W2059576607 @default.
• W2894140384 cites W2070409582 @default.
• W2894140384 cites W2078425139 @default.
• W2894140384 cites W2095655760 @default.
• W2894140384 cites W2105275554 @default.
• W2894140384 cites W2170224359 @default.
• W2894140384 cites W2330725595 @default.
• W2894140384 cites W2411854416 @default.
• W2894140384 cites W2569168468 @default.
• W2894140384 cites W2584039034 @default.
• W2894140384 cites W2605554195 @default.
• W2894140384 cites W2743037378 @default.
• W2894140384 cites W2781650718 @default.
• W2894140384 doi "https://doi.org/10.1016/j.anaerobe.2018.09.005" @default.
• W2894140384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30248446" @default.
• W2894140384 hasPublicationYear "2018" @default.
• W2894140384 type Work @default.
• W2894140384 sameAs 2894140384 @default.
• W2894140384 citedByCount "17" @default.
• W2894140384 countsByYear W28941403842019 @default.
• W2894140384 countsByYear W28941403842020 @default.
• W2894140384 countsByYear W28941403842021 @default.
• W2894140384 countsByYear W28941403842022 @default.
• W2894140384 countsByYear W28941403842023 @default.
• W2894140384 crossrefType "journal-article" @default.
• W2894140384 hasAuthorship W2894140384A5009643835 @default.
• W2894140384 hasAuthorship W2894140384A5048444823 @default.
• W2894140384 hasAuthorship W2894140384A5048999261 @default.
• W2894140384 hasAuthorship W2894140384A5051951753 @default.
• W2894140384 hasAuthorship W2894140384A5052733067 @default.
• W2894140384 hasAuthorship W2894140384A5058420371 @default.
• W2894140384 hasAuthorship W2894140384A5077327874 @default.
• W2894140384 hasAuthorship W2894140384A5078720732 @default.
• W2894140384 hasAuthorship W2894140384A5089135192 @default.
• W2894140384 hasConcept C104317684 @default.
• W2894140384 hasConcept C119128265 @default.
• W2894140384 hasConcept C153911025 @default.
• W2894140384 hasConcept C185592680 @default.
• W2894140384 hasConcept C2779116991 @default.
• W2894140384 hasConcept C43617362 @default.
• W2894140384 hasConcept C49105822 @default.
• W2894140384 hasConcept C523546767 @default.
• W2894140384 hasConcept C54355233 @default.
• W2894140384 hasConcept C552990157 @default.
• W2894140384 hasConcept C60635243 @default.
• W2894140384 hasConcept C86803240 @default.
• W2894140384 hasConcept C89423630 @default.
• W2894140384 hasConceptScore W2894140384C104317684 @default.
• W2894140384 hasConceptScore W2894140384C119128265 @default.
• W2894140384 hasConceptScore W2894140384C153911025 @default.
• W2894140384 hasConceptScore W2894140384C185592680 @default.
• W2894140384 hasConceptScore W2894140384C2779116991 @default.
• W2894140384 hasConceptScore W2894140384C43617362 @default.
• W2894140384 hasConceptScore W2894140384C49105822 @default.
• W2894140384 hasConceptScore W2894140384C523546767 @default.
• W2894140384 hasConceptScore W2894140384C54355233 @default.
• W2894140384 hasConceptScore W2894140384C552990157 @default.
• W2894140384 hasConceptScore W2894140384C60635243 @default.
• W2894140384 hasConceptScore W2894140384C86803240 @default.
• W2894140384 hasConceptScore W2894140384C89423630 @default.
• W2894140384 hasLocation W28941403841 @default.
• W2894140384 hasLocation W28941403842 @default.
• W2894140384 hasOpenAccess W2894140384 @default.
• W2894140384 hasPrimaryLocation W28941403841 @default.
• W2894140384 hasRelatedWork W1980714464 @default.
• W2894140384 hasRelatedWork W2043849936 @default.
• W2894140384 hasRelatedWork W2300579724 @default.
• W2894140384 hasRelatedWork W2369057959 @default.
• W2894140384 hasRelatedWork W2377082422 @default.
• W2894140384 hasRelatedWork W2378991221 @default.
• W2894140384 hasRelatedWork W2387800053 @default.
• W2894140384 hasRelatedWork W2411854416 @default.

• next