Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894150418> ?p ?o ?g. }
- W2894150418 endingPage "2957" @default.
- W2894150418 startingPage "2957" @default.
- W2894150418 abstract "Increased growth and proliferation of distal pulmonary artery vascular smooth muscle cells (PAVSMC) is an important pathological component of pulmonary arterial hypertension (PAH). Transforming Growth Factor-β (TGF-β) superfamily plays a critical role in PAH, but relative impacts of self-secreted Activin A, Gremlin1, and TGF-β on PAH PAVSMC growth and proliferation are not studied. Here we report that hyper-proliferative human PAH PAVSMC have elevated secretion of TGF-β1 and, to a lesser extent, Activin A, but not Gremlin 1, and significantly reduced Ser465/467-Smad2 and Ser423/425-Smad3 phosphorylation compared to controls. Media, conditioned by PAH PAVSMC, markedly increased Ser465/467-Smad2, Ser423/425-Smad3, and Ser463/465-Smad1/5 phosphorylation, up-regulated Akt, ERK1/2, and p38 MAPK, and induced significant proliferation of non-diseased PAVSMC. Inhibitory anti-Activin A antibody reduced PAH PAVSMC growth without affecting canonical (Smads) or non-canonical (Akt, ERK1/2, p38 MAPK) effectors. Inhibitory anti-TGF-β antibody significantly reduced P-Smad3, P-ERK1/2 and proliferation of PAH PAVSMC, while anti-Gremlin 1 had no anti-proliferative effect. PDGF-BB diminished inhibitory effects of anti-Activin A and anti-TGF-β antibodies. None of the antibodies affected growth and proliferation of non-diseased PAVSMC induced by PAH PAVSMC-secreted factors. Together, these data demonstrate that human PAH PAVSMC have secretory, proliferative phenotype that could be targeted by anti-Activin A and anti-TGF-β antibodies; potential cross-talk with PDGF-BB should be considered while developing therapeutic interventions." @default.
- W2894150418 created "2018-10-05" @default.
- W2894150418 creator A5008199257 @default.
- W2894150418 creator A5014353933 @default.
- W2894150418 creator A5032767791 @default.
- W2894150418 creator A5033608225 @default.
- W2894150418 creator A5073945824 @default.
- W2894150418 creator A5077983502 @default.
- W2894150418 creator A5082764369 @default.
- W2894150418 date "2018-09-28" @default.
- W2894150418 modified "2023-10-14" @default.
- W2894150418 title "Inhibitory Antibodies against Activin A and TGF-β Reduce Self-Supported, but Not Soluble Factors-Induced Growth of Human Pulmonary Arterial Vascular Smooth Muscle Cells in Pulmonary Arterial Hypertension" @default.
- W2894150418 cites W1574697834 @default.
- W2894150418 cites W1606441445 @default.
- W2894150418 cites W1910386836 @default.
- W2894150418 cites W1929000169 @default.
- W2894150418 cites W1953539082 @default.
- W2894150418 cites W1973804253 @default.
- W2894150418 cites W1980369799 @default.
- W2894150418 cites W1984500131 @default.
- W2894150418 cites W1989343086 @default.
- W2894150418 cites W1993551856 @default.
- W2894150418 cites W1994738588 @default.
- W2894150418 cites W1998123371 @default.
- W2894150418 cites W2009629707 @default.
- W2894150418 cites W2017287650 @default.
- W2894150418 cites W2020864519 @default.
- W2894150418 cites W2021218223 @default.
- W2894150418 cites W2025593289 @default.
- W2894150418 cites W2035992245 @default.
- W2894150418 cites W2038304860 @default.
- W2894150418 cites W2039543809 @default.
- W2894150418 cites W2040599383 @default.
- W2894150418 cites W2044609448 @default.
- W2894150418 cites W2048369516 @default.
- W2894150418 cites W2071863456 @default.
- W2894150418 cites W2081276164 @default.
- W2894150418 cites W2085040665 @default.
- W2894150418 cites W2090878184 @default.
- W2894150418 cites W2096315216 @default.
- W2894150418 cites W2101275731 @default.
- W2894150418 cites W2102344094 @default.
- W2894150418 cites W2112959275 @default.
- W2894150418 cites W2113813396 @default.
- W2894150418 cites W2116057774 @default.
- W2894150418 cites W2117339256 @default.
- W2894150418 cites W2119578597 @default.
- W2894150418 cites W2128512660 @default.
- W2894150418 cites W2134915108 @default.
- W2894150418 cites W2139437349 @default.
- W2894150418 cites W2140319297 @default.
- W2894150418 cites W2146888695 @default.
- W2894150418 cites W2147944062 @default.
- W2894150418 cites W2153638986 @default.
- W2894150418 cites W2165928579 @default.
- W2894150418 cites W2170779936 @default.
- W2894150418 cites W2171273257 @default.
- W2894150418 cites W2172217343 @default.
- W2894150418 cites W2217868403 @default.
- W2894150418 cites W2218524235 @default.
- W2894150418 cites W2271349749 @default.
- W2894150418 cites W2298633694 @default.
- W2894150418 cites W2310088448 @default.
- W2894150418 cites W2337592013 @default.
- W2894150418 cites W2344324308 @default.
- W2894150418 cites W2344643399 @default.
- W2894150418 cites W2508927756 @default.
- W2894150418 cites W2518911298 @default.
- W2894150418 cites W2522325334 @default.
- W2894150418 cites W2592714610 @default.
- W2894150418 cites W2600452401 @default.
- W2894150418 cites W2603492845 @default.
- W2894150418 cites W2616160118 @default.
- W2894150418 cites W2728443108 @default.
- W2894150418 cites W2766130388 @default.
- W2894150418 cites W2789836217 @default.
- W2894150418 cites W2794636105 @default.
- W2894150418 cites W2799601188 @default.
- W2894150418 cites W1567349574 @default.
- W2894150418 doi "https://doi.org/10.3390/ijms19102957" @default.
- W2894150418 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6212879" @default.
- W2894150418 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30274147" @default.
- W2894150418 hasPublicationYear "2018" @default.
- W2894150418 type Work @default.
- W2894150418 sameAs 2894150418 @default.
- W2894150418 citedByCount "18" @default.
- W2894150418 countsByYear W28941504182019 @default.
- W2894150418 countsByYear W28941504182020 @default.
- W2894150418 countsByYear W28941504182021 @default.
- W2894150418 countsByYear W28941504182022 @default.
- W2894150418 countsByYear W28941504182023 @default.
- W2894150418 crossrefType "journal-article" @default.
- W2894150418 hasAuthorship W2894150418A5008199257 @default.
- W2894150418 hasAuthorship W2894150418A5014353933 @default.
- W2894150418 hasAuthorship W2894150418A5032767791 @default.
- W2894150418 hasAuthorship W2894150418A5033608225 @default.
- W2894150418 hasAuthorship W2894150418A5073945824 @default.
- W2894150418 hasAuthorship W2894150418A5077983502 @default.