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- W2894224245 abstract "Conventional propagation of Anthurium (Anthurium andraeanum [Lind.]) plant through seeds and suckers is a tedious and very slow process and not practical when carried out commercially on large scale. Hence, biotechnological approach such as micropropagation is considered as a novel method for production of pathogen-free true-to-type plantlets at a faster rate. In the present study, a proficient surface sterilization procedure was standardized for aseptic culture from leaf explants of Anthurium cv. Xavia, under in-vitro conditions. The leaf explants were exposed to 0.1% HgCl2 for 2, 3, 4, 5, 6, 7 and 8 min) and control (distilled water) were cultured on MS media. The leaf explants exposed for 5 minutes resulted in minimum fungal (0.00%) and bacterial (0.18%) contamination with maximum survival percentage (97.66%) at 60 days after inoculation. The most effective surface sterilization time was taken into consideration for further sterilization explants and in-vitro culture in the MS basal media supplemented with of bio-regulators along with control for production of pathogen-free callus development of anthurium." @default.
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- W2894224245 date "2018-09-10" @default.
- W2894224245 modified "2023-09-26" @default.
- W2894224245 title "Impact of Sterilization Exposure Time on Leaf Explants of Anthurium cv. Xavia for Establishing an Aseptic Culture" @default.
- W2894224245 doi "https://doi.org/10.20546/ijcmas.2018.709.354" @default.
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