FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894420996> ?p ?o ?g. }

• W2894420996 abstract "Cleavage and polyadenylation (C/P) of mRNA is an important cellular process that promotes increased diversity of mRNA isoforms and could change their stability in different cell types. The cleavage stimulation factor (CstF) complex, part of the C/P machinery, binds to U- and GU-rich sequences located downstream from the cleavage site through its RNA-binding subunit, CstF-64. Less is known about the function of the other two subunits of CstF, CstF-77 and CstF-50. Here, we show that the carboxy-terminus of CstF-77 plays a previously unrecognized role in enhancing C/P by altering how the RNA recognition motif (RRM) of CstF-64 binds RNA. In support of this finding, we also show that CstF-64 relies on CstF-77 to be transported to the nucleus; excess CstF-64 localizes to the cytoplasm, possibly via interaction with cytoplasmic RNAs. Reverse genetics and nuclear magnetic resonance studies of recombinant CstF-64 (RRM-Hinge) and CstF-77 (monkeytail-carboxy-terminal domain) indicate that the last 30 amino acids of CstF-77 increases the stability of the RRM, thus altering the affinity of the complex for RNA. These results provide new insights into the mechanism by which CstF regulates the location of the RNA cleavage site during C/P." @default.
• W2894420996 created "2018-10-05" @default.
• W2894420996 creator A5020647459 @default.
• W2894420996 creator A5062624248 @default.
• W2894420996 creator A5068683652 @default.
• W2894420996 creator A5090774479 @default.
• W2894420996 date "2018-09-26" @default.
• W2894420996 modified "2023-09-26" @default.
• W2894420996 title "The structural basis of CstF-77 modulation of cleavage and polyadenylation through stimulation of CstF-64 activity" @default.
• W2894420996 cites W1488258440 @default.
• W2894420996 cites W1562998360 @default.
• W2894420996 cites W1749659233 @default.
• W2894420996 cites W1791309331 @default.
• W2894420996 cites W1882743565 @default.
• W2894420996 cites W1967109444 @default.
• W2894420996 cites W1972208485 @default.
• W2894420996 cites W1990401457 @default.
• W2894420996 cites W1993719557 @default.
• W2894420996 cites W1998953110 @default.
• W2894420996 cites W2000666866 @default.
• W2894420996 cites W2005764753 @default.
• W2894420996 cites W2005964874 @default.
• W2894420996 cites W2009240297 @default.
• W2894420996 cites W2010088308 @default.
• W2894420996 cites W2010907792 @default.
• W2894420996 cites W2015562197 @default.
• W2894420996 cites W2017392124 @default.
• W2894420996 cites W2025079047 @default.
• W2894420996 cites W2027897447 @default.
• W2894420996 cites W2030608812 @default.
• W2894420996 cites W2032009534 @default.
• W2894420996 cites W2032749894 @default.
• W2894420996 cites W2035282527 @default.
• W2894420996 cites W2036065307 @default.
• W2894420996 cites W2038256325 @default.
• W2894420996 cites W2043076293 @default.
• W2894420996 cites W2043664938 @default.
• W2894420996 cites W2045709965 @default.
• W2894420996 cites W2047854594 @default.
• W2894420996 cites W2047935781 @default.
• W2894420996 cites W2053998703 @default.
• W2894420996 cites W2054084236 @default.
• W2894420996 cites W2061163695 @default.
• W2894420996 cites W2068227670 @default.
• W2894420996 cites W2069922747 @default.
• W2894420996 cites W2071749819 @default.
• W2894420996 cites W2072105385 @default.
• W2894420996 cites W2073350644 @default.
• W2894420996 cites W2075813184 @default.
• W2894420996 cites W2076184693 @default.
• W2894420996 cites W2078558208 @default.
• W2894420996 cites W2079932405 @default.
• W2894420996 cites W2081617495 @default.
• W2894420996 cites W2090881230 @default.
• W2894420996 cites W2094281214 @default.
• W2894420996 cites W2094782385 @default.
• W2894420996 cites W2095930993 @default.
• W2894420996 cites W2096186501 @default.
• W2894420996 cites W2099915768 @default.
• W2894420996 cites W2101975376 @default.
• W2894420996 cites W2106369798 @default.
• W2894420996 cites W2110875913 @default.
• W2894420996 cites W2114476704 @default.
• W2894420996 cites W2119875500 @default.
• W2894420996 cites W2123561365 @default.
• W2894420996 cites W2124433979 @default.
• W2894420996 cites W2128407129 @default.
• W2894420996 cites W2131824470 @default.
• W2894420996 cites W2144688507 @default.
• W2894420996 cites W2150069017 @default.
• W2894420996 cites W2151549812 @default.
• W2894420996 cites W2151562469 @default.
• W2894420996 cites W2155085170 @default.
• W2894420996 cites W2157861699 @default.
• W2894420996 cites W2162816788 @default.
• W2894420996 cites W2166494447 @default.
• W2894420996 cites W2166640436 @default.
• W2894420996 cites W2169707043 @default.
• W2894420996 cites W2169821755 @default.
• W2894420996 cites W2171258458 @default.
• W2894420996 cites W2229032859 @default.
• W2894420996 cites W2396047437 @default.
• W2894420996 cites W2461965332 @default.
• W2894420996 cites W2480300184 @default.
• W2894420996 cites W2480709584 @default.
• W2894420996 cites W2525507785 @default.
• W2894420996 cites W2530453716 @default.
• W2894420996 cites W2548124098 @default.
• W2894420996 cites W2768268160 @default.
• W2894420996 cites W2775755225 @default.
• W2894420996 cites W2793640207 @default.
• W2894420996 cites W30334872 @default.
• W2894420996 cites W70671871 @default.
• W2894420996 doi "https://doi.org/10.1093/nar/gky862" @default.
• W2894420996 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6294498" @default.
• W2894420996 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30257008" @default.
• W2894420996 hasPublicationYear "2018" @default.
• W2894420996 type Work @default.
• W2894420996 sameAs 2894420996 @default.
• W2894420996 citedByCount "9" @default.

• next