Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894425317> ?p ?o ?g. }
- W2894425317 endingPage "85" @default.
- W2894425317 startingPage "65" @default.
- W2894425317 abstract "Fibroblast activation protein-alpha (FAP) is a cell-surface transmembrane-anchored dimeric protease. This unique, constitutively active serine protease has both dipeptidyl aminopeptidase and endopeptidase activities and can hydrolyze the post-proline bond. FAP expression is very low in adult organs but is upregulated by activated fibroblasts in sites of tissue remodeling, including fibrosis, atherosclerosis, arthritis and tumors. To identify the endogenous substrates of FAP, we immortalized primary mouse embryonic fibroblasts (MEFs) from FAP gene knockout embryos and then stably transduced them to express either enzymatically active or inactive FAP. The MEF secretomes were then analyzed using degradomic and proteomic techniques. Terminal amine isotopic labeling of substrates (TAILS)-based degradomics identified cleavage sites in collagens, many other extracellular matrix (ECM) and associated proteins, and lysyl oxidase-like-1, CXCL-5, CSF-1, and C1qT6, that were confirmed in vitro In addition, differential metabolic labeling coupled with quantitative proteomic analysis also implicated FAP in ECM-cell interactions, as well as with coagulation, metabolism and wound healing associated proteins. Plasma from FAP-deficient mice exhibited slower than wild-type clotting times. This study provides a significant expansion of the substrate repertoire of FAP and provides insight into the physiological and potential pathological roles of this enigmatic protease." @default.
- W2894425317 created "2018-10-05" @default.
- W2894425317 creator A5004620625 @default.
- W2894425317 creator A5006947382 @default.
- W2894425317 creator A5025622223 @default.
- W2894425317 creator A5034727603 @default.
- W2894425317 creator A5040587398 @default.
- W2894425317 creator A5041718280 @default.
- W2894425317 creator A5047870401 @default.
- W2894425317 creator A5050843400 @default.
- W2894425317 creator A5052215482 @default.
- W2894425317 creator A5059379839 @default.
- W2894425317 creator A5066922986 @default.
- W2894425317 creator A5067004305 @default.
- W2894425317 creator A5078157555 @default.
- W2894425317 creator A5079119616 @default.
- W2894425317 creator A5085342419 @default.
- W2894425317 creator A5063493885 @default.
- W2894425317 date "2019-01-01" @default.
- W2894425317 modified "2023-10-14" @default.
- W2894425317 title "Identification of Novel Natural Substrates of Fibroblast Activation Protein-alpha by Differential Degradomics and Proteomics" @default.
- W2894425317 cites W1490344763 @default.
- W2894425317 cites W1499600962 @default.
- W2894425317 cites W1522378176 @default.
- W2894425317 cites W1533538364 @default.
- W2894425317 cites W1545804167 @default.
- W2894425317 cites W1789130462 @default.
- W2894425317 cites W1797925867 @default.
- W2894425317 cites W1821971821 @default.
- W2894425317 cites W1880934458 @default.
- W2894425317 cites W1927852927 @default.
- W2894425317 cites W1972764157 @default.
- W2894425317 cites W1974377953 @default.
- W2894425317 cites W1978887710 @default.
- W2894425317 cites W1979338861 @default.
- W2894425317 cites W1988976004 @default.
- W2894425317 cites W1992915466 @default.
- W2894425317 cites W2003236418 @default.
- W2894425317 cites W2003562059 @default.
- W2894425317 cites W2003619028 @default.
- W2894425317 cites W2010812762 @default.
- W2894425317 cites W2014752437 @default.
- W2894425317 cites W2015556811 @default.
- W2894425317 cites W2021231548 @default.
- W2894425317 cites W2023010129 @default.
- W2894425317 cites W2027290833 @default.
- W2894425317 cites W2027440025 @default.
- W2894425317 cites W2027974246 @default.
- W2894425317 cites W2041686750 @default.
- W2894425317 cites W2042568444 @default.
- W2894425317 cites W2045756246 @default.
- W2894425317 cites W2046208657 @default.
- W2894425317 cites W2047764904 @default.
- W2894425317 cites W2051795211 @default.
- W2894425317 cites W2052777370 @default.
- W2894425317 cites W2053963714 @default.
- W2894425317 cites W2055801606 @default.
- W2894425317 cites W2057465207 @default.
- W2894425317 cites W2060410847 @default.
- W2894425317 cites W2065447632 @default.
- W2894425317 cites W2065579911 @default.
- W2894425317 cites W2071601558 @default.
- W2894425317 cites W2074912245 @default.
- W2894425317 cites W2077759978 @default.
- W2894425317 cites W2078253053 @default.
- W2894425317 cites W2081098333 @default.
- W2894425317 cites W2082444360 @default.
- W2894425317 cites W2087766713 @default.
- W2894425317 cites W2087801171 @default.
- W2894425317 cites W2092420477 @default.
- W2894425317 cites W2093439119 @default.
- W2894425317 cites W2100433758 @default.
- W2894425317 cites W2100902863 @default.
- W2894425317 cites W2101545809 @default.
- W2894425317 cites W2112061542 @default.
- W2894425317 cites W2112078820 @default.
- W2894425317 cites W2117135532 @default.
- W2894425317 cites W2120202914 @default.
- W2894425317 cites W2127100488 @default.
- W2894425317 cites W2127197216 @default.
- W2894425317 cites W2128953247 @default.
- W2894425317 cites W2130706354 @default.
- W2894425317 cites W2146103026 @default.
- W2894425317 cites W2146512944 @default.
- W2894425317 cites W2152863656 @default.
- W2894425317 cites W2164118220 @default.
- W2894425317 cites W2164963986 @default.
- W2894425317 cites W2166041923 @default.
- W2894425317 cites W2167297718 @default.
- W2894425317 cites W2171431555 @default.
- W2894425317 cites W2171918098 @default.
- W2894425317 cites W2179373669 @default.
- W2894425317 cites W2181676094 @default.
- W2894425317 cites W2191178383 @default.
- W2894425317 cites W2207283323 @default.
- W2894425317 cites W2261682429 @default.
- W2894425317 cites W2282926206 @default.
- W2894425317 cites W2292257555 @default.