Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894425910> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2894425910 endingPage "407" @default.
- W2894425910 startingPage "402" @default.
- W2894425910 abstract "The composition of cerebrospinal fluid (CSF) is an invaluable parameter in better understanding of cellular and molecular processes within the mammalian brain. However, the collection of significant volumes of clean CSF can be technically challenging in studies with laboratory mice. Over the past five decades, several approaches have been developed to maximize the quantity and quality of CSF samples, either from live or euthanized animals. Due to the small amounts collected, samples from single mice were often pooled or diluted to meet volume requirements of automated counters and multiple assays.This paper reviews previous work on CSF collection in mice, thus providing methodological background for the current post-mortem procedure. This modified cisternal puncture method involves the use of a peristaltic pump for consistent and slow intracardiac perfusion, as well as a loupe headset with a custom-made glass pipette for piercing a single hole in the atlanto-occipital membrane during repeated CSF draws. Sample cleanness is verified by comparing the colour of the glass pipette and the bottom of centrifuged PCR vial against a white background.With three trained experimenters, the entire procedure (including anesthesia) takes ∼11-13 min and often results in the collection of up to 40 μl of clean CSF from males of different murine strains. Properly staggered collections allow processing of relatively large cohorts of mice per day.This modification of previously employed methods can be used in studies that require tightly-timed collections of larger volumes of undiluted, tissue-free CSF and/or individual data records." @default.
- W2894425910 created "2018-10-05" @default.
- W2894425910 creator A5030442312 @default.
- W2894425910 date "2019-01-01" @default.
- W2894425910 modified "2023-10-16" @default.
- W2894425910 title "Cerebrospinal fluid collection in laboratory mice: Literature review and modified cisternal puncture method" @default.
- W2894425910 cites W1481551714 @default.
- W2894425910 cites W1588685142 @default.
- W2894425910 cites W1817515827 @default.
- W2894425910 cites W1966601221 @default.
- W2894425910 cites W2007160112 @default.
- W2894425910 cites W2033750371 @default.
- W2894425910 cites W2043307304 @default.
- W2894425910 cites W2062279867 @default.
- W2894425910 cites W2063355653 @default.
- W2894425910 cites W2065894043 @default.
- W2894425910 cites W2070630574 @default.
- W2894425910 cites W2120222847 @default.
- W2894425910 cites W2159462479 @default.
- W2894425910 cites W2472182922 @default.
- W2894425910 cites W2773427194 @default.
- W2894425910 doi "https://doi.org/10.1016/j.jneumeth.2018.09.025" @default.
- W2894425910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30273638" @default.
- W2894425910 hasPublicationYear "2019" @default.
- W2894425910 type Work @default.
- W2894425910 sameAs 2894425910 @default.
- W2894425910 citedByCount "16" @default.
- W2894425910 countsByYear W28944259102020 @default.
- W2894425910 countsByYear W28944259102021 @default.
- W2894425910 countsByYear W28944259102022 @default.
- W2894425910 countsByYear W28944259102023 @default.
- W2894425910 crossrefType "journal-article" @default.
- W2894425910 hasAuthorship W2894425910A5030442312 @default.
- W2894425910 hasConcept C121332964 @default.
- W2894425910 hasConcept C134519083 @default.
- W2894425910 hasConcept C136229726 @default.
- W2894425910 hasConcept C142724271 @default.
- W2894425910 hasConcept C147789679 @default.
- W2894425910 hasConcept C151552171 @default.
- W2894425910 hasConcept C153294291 @default.
- W2894425910 hasConcept C185592680 @default.
- W2894425910 hasConcept C2779651940 @default.
- W2894425910 hasConcept C43617362 @default.
- W2894425910 hasConcept C71924100 @default.
- W2894425910 hasConcept C72092618 @default.
- W2894425910 hasConceptScore W2894425910C121332964 @default.
- W2894425910 hasConceptScore W2894425910C134519083 @default.
- W2894425910 hasConceptScore W2894425910C136229726 @default.
- W2894425910 hasConceptScore W2894425910C142724271 @default.
- W2894425910 hasConceptScore W2894425910C147789679 @default.
- W2894425910 hasConceptScore W2894425910C151552171 @default.
- W2894425910 hasConceptScore W2894425910C153294291 @default.
- W2894425910 hasConceptScore W2894425910C185592680 @default.
- W2894425910 hasConceptScore W2894425910C2779651940 @default.
- W2894425910 hasConceptScore W2894425910C43617362 @default.
- W2894425910 hasConceptScore W2894425910C71924100 @default.
- W2894425910 hasConceptScore W2894425910C72092618 @default.
- W2894425910 hasFunder F4320321802 @default.
- W2894425910 hasFunder F4320334506 @default.
- W2894425910 hasLocation W28944259101 @default.
- W2894425910 hasLocation W28944259102 @default.
- W2894425910 hasOpenAccess W2894425910 @default.
- W2894425910 hasPrimaryLocation W28944259101 @default.
- W2894425910 hasRelatedWork W2049275484 @default.
- W2894425910 hasRelatedWork W2050237276 @default.
- W2894425910 hasRelatedWork W2067556967 @default.
- W2894425910 hasRelatedWork W2142352611 @default.
- W2894425910 hasRelatedWork W2215150368 @default.
- W2894425910 hasRelatedWork W2588034663 @default.
- W2894425910 hasRelatedWork W2748952813 @default.
- W2894425910 hasRelatedWork W2894425910 @default.
- W2894425910 hasRelatedWork W2899084033 @default.
- W2894425910 hasRelatedWork W2740168187 @default.
- W2894425910 hasVolume "311" @default.
- W2894425910 isParatext "false" @default.
- W2894425910 isRetracted "false" @default.
- W2894425910 magId "2894425910" @default.
- W2894425910 workType "article" @default.