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• W2894426566 abstract "O-linked beta-N-acetylglucosamine (O-GlcNAc) is an abundant and essential intracellular form of protein glycosylation in animals and plants. In humans, dysregulation of O-GlcNAcylation occurs in a wide range of diseases, including cancer, diabetes and neurodegeneration. Since its discovery more than thirty years ago, great strides have been made in understanding central aspects of O-GlcNAc signaling, including identifying thousands of its substrates and characterizing the enzymes that govern it. However, while many O-GlcNAcylated proteins have been reported, only a small subset of these change their glycosylation status in response to a typical stimulus or stress. Identifying the functionally important O-GlcNAcylation changes in any given signaling context remains a significant challenge in the field. To address this need, we leveraged chemical biology and quantitative mass spectrometry methods to create a new glycoproteomics workflow for profiling stimulus-dependent changes in O-GlcNAcylated proteins. In proof-of-principle experiments, we used this new workflow to interrogate changes in O-GlcNAc substrates in mammalian protein trafficking pathways. Interestingly, our results revealed dynamic O-GlcNAcylation of COPgamma1, an essential component of the coat protein I (COPI) complex that mediates Golgi protein trafficking. Moreover, we detected eleven O-GlcNAc moieties on COPgamma1 and found that this modification is reduced by a model secretory stress that halts COPI trafficking. Our results suggest that O-GlcNAcylation may regulate the mammalian COPI system, analogous to its previously reported roles in other protein trafficking pathways. More broadly, our glycoproteomics workflow is applicable to myriad systems and stimuli, empowering future studies of O-GlcNAc in a host of biological contexts." @default.
• W2894426566 created "2018-10-05" @default.
• W2894426566 creator A5010334212 @default.
• W2894426566 creator A5027125366 @default.
• W2894426566 creator A5054443109 @default.
• W2894426566 creator A5055915667 @default.
• W2894426566 creator A5061016015 @default.
• W2894426566 creator A5075691739 @default.
• W2894426566 date "2018-10-15" @default.
• W2894426566 modified "2023-10-02" @default.
• W2894426566 title "A Novel Glycoproteomics Workflow Reveals Dynamic O-GlcNAcylation of COPγ1 as a Candidate Regulator of Protein Trafficking" @default.
• W2894426566 cites W1504722292 @default.
• W2894426566 cites W1554689987 @default.
• W2894426566 cites W1579403622 @default.
• W2894426566 cites W1582195464 @default.
• W2894426566 cites W1593869902 @default.
• W2894426566 cites W1874398063 @default.
• W2894426566 cites W1878616527 @default.
• W2894426566 cites W1895538144 @default.
• W2894426566 cites W1902362655 @default.
• W2894426566 cites W1941254014 @default.
• W2894426566 cites W1964541142 @default.
• W2894426566 cites W1965355119 @default.
• W2894426566 cites W1968652549 @default.
• W2894426566 cites W1973630895 @default.
• W2894426566 cites W1974515407 @default.
• W2894426566 cites W1982922156 @default.
• W2894426566 cites W1984118759 @default.
• W2894426566 cites W1990349821 @default.
• W2894426566 cites W1993052072 @default.
• W2894426566 cites W1995490800 @default.
• W2894426566 cites W1996310767 @default.
• W2894426566 cites W1996930443 @default.
• W2894426566 cites W2001156696 @default.
• W2894426566 cites W2002085911 @default.
• W2894426566 cites W2002860607 @default.
• W2894426566 cites W2004373930 @default.
• W2894426566 cites W2005672588 @default.
• W2894426566 cites W2006296741 @default.
• W2894426566 cites W2007072101 @default.
• W2894426566 cites W2007550555 @default.
• W2894426566 cites W2008801986 @default.
• W2894426566 cites W2013020574 @default.
• W2894426566 cites W2017548268 @default.
• W2894426566 cites W2017614262 @default.
• W2894426566 cites W2019642182 @default.
• W2894426566 cites W2020044636 @default.
• W2894426566 cites W2021597697 @default.
• W2894426566 cites W2029152767 @default.
• W2894426566 cites W2029439166 @default.
• W2894426566 cites W2029489645 @default.
• W2894426566 cites W2032811916 @default.
• W2894426566 cites W2033608921 @default.
• W2894426566 cites W2034457197 @default.
• W2894426566 cites W2035011858 @default.
• W2894426566 cites W2038422914 @default.
• W2894426566 cites W2040714286 @default.
• W2894426566 cites W2043274355 @default.
• W2894426566 cites W2043528950 @default.
• W2894426566 cites W2044873332 @default.
• W2894426566 cites W2046236732 @default.
• W2894426566 cites W2047096855 @default.
• W2894426566 cites W2047865754 @default.
• W2894426566 cites W2050149304 @default.
• W2894426566 cites W2050554863 @default.
• W2894426566 cites W2051246945 @default.
• W2894426566 cites W2052135200 @default.
• W2894426566 cites W2059725389 @default.
• W2894426566 cites W2060744329 @default.
• W2894426566 cites W2061011762 @default.
• W2894426566 cites W2063971860 @default.
• W2894426566 cites W2067486037 @default.
• W2894426566 cites W2070859810 @default.
• W2894426566 cites W2071520055 @default.
• W2894426566 cites W2071962567 @default.
• W2894426566 cites W2072235312 @default.
• W2894426566 cites W2073750692 @default.
• W2894426566 cites W2073897773 @default.
• W2894426566 cites W2074437836 @default.
• W2894426566 cites W2078330387 @default.
• W2894426566 cites W2078959581 @default.
• W2894426566 cites W2080425738 @default.
• W2894426566 cites W2081446575 @default.
• W2894426566 cites W2081500927 @default.
• W2894426566 cites W2083510841 @default.
• W2894426566 cites W2089198803 @default.
• W2894426566 cites W2090211150 @default.
• W2894426566 cites W2091910468 @default.
• W2894426566 cites W2096306761 @default.
• W2894426566 cites W2096443403 @default.
• W2894426566 cites W2096849837 @default.
• W2894426566 cites W2098687851 @default.
• W2894426566 cites W2100617913 @default.
• W2894426566 cites W2101298061 @default.
• W2894426566 cites W2104496885 @default.
• W2894426566 cites W2105548732 @default.
• W2894426566 cites W2106412012 @default.
• W2894426566 cites W2107089340 @default.
• W2894426566 cites W2107312415 @default.
• W2894426566 cites W2107601138 @default.

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