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• W2894442776 abstract "The short chain dehydrogenase/reductase superfamily (SDR) is a large family of NAD(P)H-dependent enzymes found in all kingdoms of life. SDRs are particularly well-represented in plants, playing diverse roles in both primary and secondary metabolism. In addition, some plant SDRs are also able to catalyse a reductive cyclisation reaction critical for the biosynthesis of the iridoid backbone that contains a fused 5 and 6-membered ring scaffold. Mining the EST database of Plantago major, a medicinal plant that makes iridoids, we identified a putative 5β-progesterone reductase gene, PmMOR (P. major multisubstrate oxido-reductase), that is 60% identical to the iridoid synthase gene from Catharanthus roseus. The PmMOR protein was recombinantly expressed and its enzymatic activity assayed against three putative substrates, 8-oxogeranial, citral and progesterone. The enzyme demonstrated promiscuous enzymatic activity and was able to not only reduce progesterone and citral, but also to catalyse the reductive cyclisation of 8-oxogeranial. The crystal structures of PmMOR wild type and PmMOR mutants in complex with NADP+ or NAD+ and either 8-oxogeranial, citral or progesterone help to reveal the substrate specificity determinants and catalytic machinery of the protein. Site-directed mutagenesis studies were performed and provide a foundation for understanding the promiscuous activity of the enzyme." @default.
• W2894442776 created "2018-10-05" @default.
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• W2894442776 date "2018-10-04" @default.
• W2894442776 modified "2023-10-18" @default.
• W2894442776 title "A multisubstrate reductase from Plantago major: structure-function in the short chain reductase superfamily" @default.
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• W2894442776 doi "https://doi.org/10.1038/s41598-018-32967-1" @default.
• W2894442776 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6172241" @default.
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• W2894442776 hasPublicationYear "2018" @default.
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