FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894443893> ?p ?o ?g. }

• W2894443893 endingPage "1552" @default.
• W2894443893 startingPage "1543" @default.
• W2894443893 abstract "Background: The oxygen transfer rate (OTR) and the biomass concentration are two important parameters describing a microbial fermentation. It has been shown before that from the course of these parameters over time information on metabolic burden during heterologous protein production can be obtained. While online monitoring in large fermenters is ubiquitously established, it is still not a common practice in small‐scale cultures. Nevertheless, several techniques like the Respiration Activity MOnitoring System (RAMOS) device for online monitoring of the OTR in shake flasks and the BioLector device for measuring scattered light (ScL) representing biomass in microtiter plates have been developed. Results: A new microtiter plate‐based method is presented that reveals how online derived ScL signals can be transformed into signals that are proportional to the courses of OTR over time for Escherichia coli . The transformed signal is obtained by simply taking the first derivative of ScL (dScL/dt). The proportionality of both parameters is successfully validated for the strains E. coli BL21(DE3) and Tuner(DE3) expressing cellulases and the fluorescent protein FbFP, respectively. Relative amounts of produced heterologous proteins are predicted exclusively based on the course of the transformed ScL signal. A variety of induction conditions with varying inducer concentration and induction time were investigated with this method. Conclusion: The presented method based on ScL measurement allows for high‐throughput online determination of signals proportional to OTR courses. They enable the interpretation of physiological states and offer the possibility to predict the recombinant protein production in E. coli . © 2018 American Institute of Chemical Engineers Biotechnol. Prog ., 34:1543–1552, 2018" @default.
• W2894443893 created "2018-10-05" @default.
• W2894443893 creator A5005733952 @default.
• W2894443893 creator A5022603631 @default.
• W2894443893 creator A5038521981 @default.
• W2894443893 creator A5084728461 @default.
• W2894443893 date "2018-09-24" @default.
• W2894443893 modified "2023-10-18" @default.
• W2894443893 title "Prediction of recombinant protein production by <i>Escherichia coli</i> derived online from indicators of metabolic burden" @default.
• W2894443893 cites W1971481340 @default.
• W2894443893 cites W1975993749 @default.
• W2894443893 cites W1981454413 @default.
• W2894443893 cites W1984442965 @default.
• W2894443893 cites W1990785657 @default.
• W2894443893 cites W2010774343 @default.
• W2894443893 cites W2041733552 @default.
• W2894443893 cites W2045439749 @default.
• W2894443893 cites W2050648925 @default.
• W2894443893 cites W2052180068 @default.
• W2894443893 cites W2055059689 @default.
• W2894443893 cites W2073134373 @default.
• W2894443893 cites W2081294502 @default.
• W2894443893 cites W2081544371 @default.
• W2894443893 cites W2090084497 @default.
• W2894443893 cites W2090884527 @default.
• W2894443893 cites W2092255762 @default.
• W2894443893 cites W2101020258 @default.
• W2894443893 cites W2103158467 @default.
• W2894443893 cites W2104470545 @default.
• W2894443893 cites W2106943551 @default.
• W2894443893 cites W2110039658 @default.
• W2894443893 cites W2116253414 @default.
• W2894443893 cites W2116904877 @default.
• W2894443893 cites W2118898625 @default.
• W2894443893 cites W2119563557 @default.
• W2894443893 cites W2133903524 @default.
• W2894443893 cites W2140018967 @default.
• W2894443893 cites W2142408930 @default.
• W2894443893 cites W2145907180 @default.
• W2894443893 cites W2149079511 @default.
• W2894443893 cites W2150637735 @default.
• W2894443893 cites W2150686726 @default.
• W2894443893 cites W2157086343 @default.
• W2894443893 cites W2158856418 @default.
• W2894443893 cites W2165183373 @default.
• W2894443893 cites W2205744264 @default.
• W2894443893 cites W2269749921 @default.
• W2894443893 cites W2280388944 @default.
• W2894443893 cites W2338430451 @default.
• W2894443893 cites W2416065856 @default.
• W2894443893 cites W2513079084 @default.
• W2894443893 cites W2534742249 @default.
• W2894443893 cites W2545950570 @default.
• W2894443893 cites W2558650716 @default.
• W2894443893 cites W2569823987 @default.
• W2894443893 cites W2607703196 @default.
• W2894443893 cites W2626491980 @default.
• W2894443893 cites W2769232385 @default.
• W2894443893 doi "https://doi.org/10.1002/btpr.2704" @default.
• W2894443893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30248250" @default.
• W2894443893 hasPublicationYear "2018" @default.
• W2894443893 type Work @default.
• W2894443893 sameAs 2894443893 @default.
• W2894443893 citedByCount "16" @default.
• W2894443893 countsByYear W28944438932019 @default.
• W2894443893 countsByYear W28944438932020 @default.
• W2894443893 countsByYear W28944438932021 @default.
• W2894443893 countsByYear W28944438932022 @default.
• W2894443893 countsByYear W28944438932023 @default.
• W2894443893 crossrefType "journal-article" @default.
• W2894443893 hasAuthorship W2894443893A5005733952 @default.
• W2894443893 hasAuthorship W2894443893A5022603631 @default.
• W2894443893 hasAuthorship W2894443893A5038521981 @default.
• W2894443893 hasAuthorship W2894443893A5084728461 @default.
• W2894443893 hasConcept C100544194 @default.
• W2894443893 hasConcept C104317684 @default.
• W2894443893 hasConcept C115540264 @default.
• W2894443893 hasConcept C139149728 @default.
• W2894443893 hasConcept C147789679 @default.
• W2894443893 hasConcept C168170006 @default.
• W2894443893 hasConcept C185592680 @default.
• W2894443893 hasConcept C186060115 @default.
• W2894443893 hasConcept C2225953 @default.
• W2894443893 hasConcept C2778665413 @default.
• W2894443893 hasConcept C2780115692 @default.
• W2894443893 hasConcept C31903555 @default.
• W2894443893 hasConcept C3675279 @default.
• W2894443893 hasConcept C40767141 @default.
• W2894443893 hasConcept C43617362 @default.
• W2894443893 hasConcept C547475151 @default.
• W2894443893 hasConcept C55493867 @default.
• W2894443893 hasConcept C59822182 @default.
• W2894443893 hasConcept C6557445 @default.
• W2894443893 hasConcept C70721500 @default.
• W2894443893 hasConcept C86803240 @default.
• W2894443893 hasConceptScore W2894443893C100544194 @default.
• W2894443893 hasConceptScore W2894443893C104317684 @default.
• W2894443893 hasConceptScore W2894443893C115540264 @default.

• next