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- W2894493467 abstract "ObjectiveTo explore a new approach to isolate the umbilical cord-mesenchymal stem cells (UC-MSCs) from a whole umbilical cord.MethodsSingle enzyme was used to digest the whole umbilical cord, and passaged and cultured, draw the growth curve, cell cycle and cell wall antigen on the surface by flow cytometry. In particular inducing system, the adherent cells were induced into adipocytes and osteoblast.ResultsThe mesenchymal stem cells (MSCs) were derived from the whole umbilical cord. The growth curve showed that the cell doubling time was (24.15 ± 0.49) h and the percentage of G1 phase cells was 82.66%. The adherent cells expressed the CD93, CD105, CD44, CD29 and CD73, and did not express the CD34, CD45, CD31 and human leukocyte antigen DR (HLA-DR). UC-MSCs could differentiate into adipocytes and osteoblast.ConclusionsSingle enzyme approach is a good method to obtain UC-MSCs from whole human umbilical cord, and it provides a theoretical basis for the establishment of MSCs bank and clinical application.Key words: Umbilical cord; Mesenchymal stem cells; Enzyme digestion" @default.
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- W2894493467 date "2017-11-05" @default.
- W2894493467 modified "2023-09-26" @default.
- W2894493467 title "Isolation and characterization of mesenchymal stem cells from whole human umbilical cord with a single enzyme approach" @default.
- W2894493467 doi "https://doi.org/10.3760/cma.j.issn.1673-4904.2017.11.002" @default.
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