FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894525817> ?p ?o ?g. }

• W2894525817 abstract "In the postpartum cow, early diagnosis of uterine disease is currently problematic due to the lack of reliable, non-invasive diagnostic methods. Cervico-vaginal mucus (CVM) is an easy to collect potentially informative source of biomarkers for the diagnosis and prognosis of uterine disease in cows. Here, we report an improved method for processing CVM from postpartum dairy cows for the measurement of immune biomarkers. CVM samples were collected from the vagina using gloved hand during the first two weeks postpartum and processed with buffer alone or buffer containing different concentrations of the reducing agents recommended in standard protocols: Dithiothriotol (DTT) or N-Acetyl-L-Cysteine (NAC). Total protein was measured using the bicinchoninic acid (BCA) assay; interleukin 6 (IL-6), IL-8 and α1-acid glycoprotein (AGP) were measured by ELISA. We found that use of reducing agents to liquefy CVM affects protein yield and the accuracy of biomarker detection. Our improved protocol results in lower protein yields but improved detection of cytokines and chemokines. Using our modified method to measure AGP in CVM we found raised levels of AGP at seven days postpartum in CVM from cows that went on to develop endometritis. We conclude that processing CVM without reducing agents improves detection of biomarkers that reflect uterine health in cattle. We propose that measurement of AGP in CVM during the first week postpartum may identify cows at risk of developing clinical endometritis." @default.
• W2894525817 created "2018-10-05" @default.
• W2894525817 creator A5002853430 @default.
• W2894525817 creator A5030502584 @default.
• W2894525817 creator A5033778987 @default.
• W2894525817 creator A5054395909 @default.
• W2894525817 creator A5091548602 @default.
• W2894525817 date "2018-09-29" @default.
• W2894525817 modified "2023-09-30" @default.
• W2894525817 title "Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle" @default.
• W2894525817 cites W1568072008 @default.
• W2894525817 cites W1873207210 @default.
• W2894525817 cites W1910326421 @default.
• W2894525817 cites W1968645444 @default.
• W2894525817 cites W1972719386 @default.
• W2894525817 cites W1973664201 @default.
• W2894525817 cites W1980143203 @default.
• W2894525817 cites W1987569414 @default.
• W2894525817 cites W1998409355 @default.
• W2894525817 cites W2028405416 @default.
• W2894525817 cites W2037202483 @default.
• W2894525817 cites W2040462374 @default.
• W2894525817 cites W2040560816 @default.
• W2894525817 cites W2052541235 @default.
• W2894525817 cites W2057318645 @default.
• W2894525817 cites W2076552777 @default.
• W2894525817 cites W2104453489 @default.
• W2894525817 cites W2106286901 @default.
• W2894525817 cites W2108511142 @default.
• W2894525817 cites W2112989452 @default.
• W2894525817 cites W2114819640 @default.
• W2894525817 cites W2123402700 @default.
• W2894525817 cites W2123742019 @default.
• W2894525817 cites W2123884323 @default.
• W2894525817 cites W2126272683 @default.
• W2894525817 cites W2138579860 @default.
• W2894525817 cites W2159666172 @default.
• W2894525817 cites W2741457362 @default.
• W2894525817 doi "https://doi.org/10.1186/s12917-018-1619-5" @default.
• W2894525817 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6162890" @default.
• W2894525817 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30268128" @default.
• W2894525817 hasPublicationYear "2018" @default.
• W2894525817 type Work @default.
• W2894525817 sameAs 2894525817 @default.
• W2894525817 citedByCount "9" @default.
• W2894525817 countsByYear W28945258172020 @default.
• W2894525817 countsByYear W28945258172021 @default.
• W2894525817 countsByYear W28945258172022 @default.
• W2894525817 countsByYear W28945258172023 @default.
• W2894525817 crossrefType "journal-article" @default.
• W2894525817 hasAuthorship W2894525817A5002853430 @default.
• W2894525817 hasAuthorship W2894525817A5030502584 @default.
• W2894525817 hasAuthorship W2894525817A5033778987 @default.
• W2894525817 hasAuthorship W2894525817A5054395909 @default.
• W2894525817 hasAuthorship W2894525817A5091548602 @default.
• W2894525817 hasBestOaLocation W28945258171 @default.
• W2894525817 hasConcept C126322002 @default.
• W2894525817 hasConcept C18903297 @default.
• W2894525817 hasConcept C2775846227 @default.
• W2894525817 hasConcept C2777312293 @default.
• W2894525817 hasConcept C2777910003 @default.
• W2894525817 hasConcept C2779234561 @default.
• W2894525817 hasConcept C2781197716 @default.
• W2894525817 hasConcept C3018745865 @default.
• W2894525817 hasConcept C42407357 @default.
• W2894525817 hasConcept C54355233 @default.
• W2894525817 hasConcept C55493867 @default.
• W2894525817 hasConcept C71924100 @default.
• W2894525817 hasConcept C86803240 @default.
• W2894525817 hasConceptScore W2894525817C126322002 @default.
• W2894525817 hasConceptScore W2894525817C18903297 @default.
• W2894525817 hasConceptScore W2894525817C2775846227 @default.
• W2894525817 hasConceptScore W2894525817C2777312293 @default.
• W2894525817 hasConceptScore W2894525817C2777910003 @default.
• W2894525817 hasConceptScore W2894525817C2779234561 @default.
• W2894525817 hasConceptScore W2894525817C2781197716 @default.
• W2894525817 hasConceptScore W2894525817C3018745865 @default.
• W2894525817 hasConceptScore W2894525817C42407357 @default.
• W2894525817 hasConceptScore W2894525817C54355233 @default.
• W2894525817 hasConceptScore W2894525817C55493867 @default.
• W2894525817 hasConceptScore W2894525817C71924100 @default.
• W2894525817 hasConceptScore W2894525817C86803240 @default.
• W2894525817 hasFunder F4320312041 @default.
• W2894525817 hasFunder F4320320831 @default.
• W2894525817 hasIssue "1" @default.
• W2894525817 hasLocation W28945258171 @default.
• W2894525817 hasLocation W28945258172 @default.
• W2894525817 hasLocation W28945258173 @default.
• W2894525817 hasLocation W28945258174 @default.
• W2894525817 hasLocation W28945258175 @default.
• W2894525817 hasLocation W28945258176 @default.
• W2894525817 hasLocation W28945258177 @default.
• W2894525817 hasLocation W28945258178 @default.
• W2894525817 hasOpenAccess W2894525817 @default.
• W2894525817 hasPrimaryLocation W28945258171 @default.
• W2894525817 hasRelatedWork W1987215768 @default.
• W2894525817 hasRelatedWork W1991129960 @default.
• W2894525817 hasRelatedWork W1995188757 @default.
• W2894525817 hasRelatedWork W2016679923 @default.
• W2894525817 hasRelatedWork W2102428589 @default.

• next