FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894594054> ?p ?o ?g. }

• W2894594054 abstract "As inducible protein expression plasmids available for mycobacterial species are limited, here we demonstrate the utility of an acid-inducible promoter driving gene and subsequent protein expression of a difficult to express protein. We wanted to assess the use of an acid inducible promoter expression system to produce proteins in a mycobacterial system, specifically proteins that when overexpressed interfere with mycobacterial cell growth. Overexpression of those types of proteins would require a tightly regulated promoter system. We employed the Mycobacterium tuberculosis lipF minimal acid-inducible promoter (mpr) which had previously been shown to be upregulated by acidic stress >100 X and to have a low basal level of expression in the absence of acidic stress. It is active from pH 4.3 up to pH 6.4 making this an acid range that is compatible with mycobacterial growth or survival and active at acidic ranges encountered in vivo within acidified phagosomes of macrophages. We therefore cloned the M. tuberculosis gene Rv3488, whose product had been unable to be expressed constitutively, into a plasmid downstream of the lipF mpr promoter and overexpressed this gene in the presence of acidic pH in Mycobacterium smegmatis. Sustained overexpression of the gene leads to inhibition of replication of mycobacterial cells as well as inhibition of carotenoid pigment synthesis, while short-term overexpression leads to appropriate protein expression." @default.
• W2894594054 created "2018-10-12" @default.
• W2894594054 creator A5007744522 @default.
• W2894594054 creator A5010066326 @default.
• W2894594054 creator A5030158342 @default.
• W2894594054 date "2018-11-01" @default.
• W2894594054 modified "2023-10-17" @default.
• W2894594054 title "Over expression of the constitutive expression refractory Mycobacterium tuberculosis Rv3488 protein with an acid-inducible expression system" @default.
• W2894594054 cites W1507498672 @default.
• W2894594054 cites W1964476936 @default.
• W2894594054 cites W1965794026 @default.
• W2894594054 cites W1978280219 @default.
• W2894594054 cites W1981262518 @default.
• W2894594054 cites W1983940758 @default.
• W2894594054 cites W1989883893 @default.
• W2894594054 cites W2004896223 @default.
• W2894594054 cites W2007097682 @default.
• W2894594054 cites W2021296798 @default.
• W2894594054 cites W2022472102 @default.
• W2894594054 cites W2032370182 @default.
• W2894594054 cites W2043538786 @default.
• W2894594054 cites W2049261534 @default.
• W2894594054 cites W2058004698 @default.
• W2894594054 cites W2059135418 @default.
• W2894594054 cites W2059535796 @default.
• W2894594054 cites W2063576137 @default.
• W2894594054 cites W2065550443 @default.
• W2894594054 cites W2066958748 @default.
• W2894594054 cites W2068046091 @default.
• W2894594054 cites W2069224592 @default.
• W2894594054 cites W2076129479 @default.
• W2894594054 cites W2078290231 @default.
• W2894594054 cites W2081661863 @default.
• W2894594054 cites W2092833729 @default.
• W2894594054 cites W2106759423 @default.
• W2894594054 cites W2110530857 @default.
• W2894594054 cites W2119784689 @default.
• W2894594054 cites W2123359143 @default.
• W2894594054 cites W2137814522 @default.
• W2894594054 cites W2139531099 @default.
• W2894594054 cites W2163999241 @default.
• W2894594054 cites W2168742687 @default.
• W2894594054 cites W353001910 @default.
• W2894594054 doi "https://doi.org/10.1016/j.mimet.2018.09.021" @default.
• W2894594054 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30287353" @default.
• W2894594054 hasPublicationYear "2018" @default.
• W2894594054 type Work @default.
• W2894594054 sameAs 2894594054 @default.
• W2894594054 citedByCount "0" @default.
• W2894594054 crossrefType "journal-article" @default.
• W2894594054 hasAuthorship W2894594054A5007744522 @default.
• W2894594054 hasAuthorship W2894594054A5010066326 @default.
• W2894594054 hasAuthorship W2894594054A5030158342 @default.
• W2894594054 hasConcept C101762097 @default.
• W2894594054 hasConcept C104317684 @default.
• W2894594054 hasConcept C116010114 @default.
• W2894594054 hasConcept C119113125 @default.
• W2894594054 hasConcept C142724271 @default.
• W2894594054 hasConcept C150194340 @default.
• W2894594054 hasConcept C153911025 @default.
• W2894594054 hasConcept C161478664 @default.
• W2894594054 hasConcept C165864922 @default.
• W2894594054 hasConcept C167625842 @default.
• W2894594054 hasConcept C22744801 @default.
• W2894594054 hasConcept C2777204207 @default.
• W2894594054 hasConcept C2777975735 @default.
• W2894594054 hasConcept C2780374374 @default.
• W2894594054 hasConcept C2781069245 @default.
• W2894594054 hasConcept C33161422 @default.
• W2894594054 hasConcept C40767141 @default.
• W2894594054 hasConcept C523546767 @default.
• W2894594054 hasConcept C54355233 @default.
• W2894594054 hasConcept C55493867 @default.
• W2894594054 hasConcept C71924100 @default.
• W2894594054 hasConcept C79879829 @default.
• W2894594054 hasConcept C86803240 @default.
• W2894594054 hasConceptScore W2894594054C101762097 @default.
• W2894594054 hasConceptScore W2894594054C104317684 @default.
• W2894594054 hasConceptScore W2894594054C116010114 @default.
• W2894594054 hasConceptScore W2894594054C119113125 @default.
• W2894594054 hasConceptScore W2894594054C142724271 @default.
• W2894594054 hasConceptScore W2894594054C150194340 @default.
• W2894594054 hasConceptScore W2894594054C153911025 @default.
• W2894594054 hasConceptScore W2894594054C161478664 @default.
• W2894594054 hasConceptScore W2894594054C165864922 @default.
• W2894594054 hasConceptScore W2894594054C167625842 @default.
• W2894594054 hasConceptScore W2894594054C22744801 @default.
• W2894594054 hasConceptScore W2894594054C2777204207 @default.
• W2894594054 hasConceptScore W2894594054C2777975735 @default.
• W2894594054 hasConceptScore W2894594054C2780374374 @default.
• W2894594054 hasConceptScore W2894594054C2781069245 @default.
• W2894594054 hasConceptScore W2894594054C33161422 @default.
• W2894594054 hasConceptScore W2894594054C40767141 @default.
• W2894594054 hasConceptScore W2894594054C523546767 @default.
• W2894594054 hasConceptScore W2894594054C54355233 @default.
• W2894594054 hasConceptScore W2894594054C55493867 @default.
• W2894594054 hasConceptScore W2894594054C71924100 @default.
• W2894594054 hasConceptScore W2894594054C79879829 @default.
• W2894594054 hasConceptScore W2894594054C86803240 @default.
• W2894594054 hasFunder F4320322601 @default.

• next