FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894621070> ?p ?o ?g. }

• W2894621070 abstract "Abstract Primordial germ cells (PGCs), the embryonic precursors of the sperm and egg, are used for the introduction of genetic modifications into avian genome. Introduction of small defined sequences using genome editing has not been demonstrated in bird species. Here, we compared oligonucleotide-mediated HDR using wild type SpCas9 (SpCas9-WT) and high fidelity SpCas9-HF1 in PGCs and show that many loci in chicken PGCs can be precise edited using donors containing CRISPR/Cas9-blocking mutations positioned in the protospacer adjacent motif (PAM). However, targeting was more efficient using SpCas9-HF1 when mutations were introduced only into the gRNA target sequence. We subsequently employed an eGFP-to-BFP conversion assay, to directly compare HDR mediated by SpCas9-WT and SpCas9-HF1 and discovered that SpCas9-HF1 increases HDR while reducing INDEL formation. Furthermore, SpCas9-HF1 increases the frequency of single allele editing in comparison to SpCas9-WT. We used SpCas9-HF1 to demonstrate the introduction of monoallelic and biallelic point mutations into the FGF20 gene and generate clonal populations of edited PGCs with defined homozygous and heterozygous genotypes. Our results demonstrate the use of oligonucleotide donors and high fidelity CRISPR/Cas9 variants to perform precise genome editing with high efficiency in PGCs." @default.
• W2894621070 created "2018-10-12" @default.
• W2894621070 creator A5032821975 @default.
• W2894621070 creator A5066083669 @default.
• W2894621070 creator A5086655876 @default.
• W2894621070 creator A5088678747 @default.
• W2894621070 date "2018-10-11" @default.
• W2894621070 modified "2023-10-16" @default.
• W2894621070 title "High fidelity CRISPR/Cas9 increases precise monoallelic and biallelic editing events in primordial germ cells" @default.
• W2894621070 cites W137643597 @default.
• W2894621070 cites W1968458490 @default.
• W2894621070 cites W1970894097 @default.
• W2894621070 cites W1976391205 @default.
• W2894621070 cites W1977709885 @default.
• W2894621070 cites W1984064827 @default.
• W2894621070 cites W1984919526 @default.
• W2894621070 cites W1991502013 @default.
• W2894621070 cites W1993000387 @default.
• W2894621070 cites W1998356880 @default.
• W2894621070 cites W2000046950 @default.
• W2894621070 cites W2007594606 @default.
• W2894621070 cites W2008147758 @default.
• W2894621070 cites W2010405210 @default.
• W2894621070 cites W2010711320 @default.
• W2894621070 cites W2014639769 @default.
• W2894621070 cites W2018462357 @default.
• W2894621070 cites W2024626278 @default.
• W2894621070 cites W2033493060 @default.
• W2894621070 cites W2036176224 @default.
• W2894621070 cites W2037322891 @default.
• W2894621070 cites W2039751785 @default.
• W2894621070 cites W2042647460 @default.
• W2894621070 cites W2045435533 @default.
• W2894621070 cites W2052192736 @default.
• W2894621070 cites W2055653287 @default.
• W2894621070 cites W2056204122 @default.
• W2894621070 cites W2056211814 @default.
• W2894621070 cites W2062756621 @default.
• W2894621070 cites W2075679174 @default.
• W2894621070 cites W2077659966 @default.
• W2894621070 cites W2091518382 @default.
• W2894621070 cites W2096448447 @default.
• W2894621070 cites W2099627146 @default.
• W2894621070 cites W2100303227 @default.
• W2894621070 cites W2105464583 @default.
• W2894621070 cites W2106070783 @default.
• W2894621070 cites W2108966286 @default.
• W2894621070 cites W2112444085 @default.
• W2894621070 cites W2116534141 @default.
• W2894621070 cites W2131569286 @default.
• W2894621070 cites W2133386058 @default.
• W2894621070 cites W2140380023 @default.
• W2894621070 cites W2155775409 @default.
• W2894621070 cites W2157205823 @default.
• W2894621070 cites W2158570164 @default.
• W2894621070 cites W2162098634 @default.
• W2894621070 cites W2162438177 @default.
• W2894621070 cites W2162573011 @default.
• W2894621070 cites W2162603028 @default.
• W2894621070 cites W2168588359 @default.
• W2894621070 cites W2170039745 @default.
• W2894621070 cites W2173835926 @default.
• W2894621070 cites W2177540144 @default.
• W2894621070 cites W2188223075 @default.
• W2894621070 cites W2202899490 @default.
• W2894621070 cites W2224398381 @default.
• W2894621070 cites W2260480416 @default.
• W2894621070 cites W2289992440 @default.
• W2894621070 cites W2297811838 @default.
• W2894621070 cites W2304268185 @default.
• W2894621070 cites W2315885991 @default.
• W2894621070 cites W2341245738 @default.
• W2894621070 cites W2343876552 @default.
• W2894621070 cites W2402498701 @default.
• W2894621070 cites W2414904000 @default.
• W2894621070 cites W2462730547 @default.
• W2894621070 cites W2481994947 @default.
• W2894621070 cites W2515633032 @default.
• W2894621070 cites W2517892442 @default.
• W2894621070 cites W2549900461 @default.
• W2894621070 cites W2550769902 @default.
• W2894621070 cites W2557406575 @default.
• W2894621070 cites W2571413046 @default.
• W2894621070 cites W2577940559 @default.
• W2894621070 cites W2586599127 @default.
• W2894621070 cites W2614952490 @default.
• W2894621070 cites W2623580087 @default.
• W2894621070 cites W2624718752 @default.
• W2894621070 cites W2726053863 @default.
• W2894621070 cites W2727344742 @default.
• W2894621070 cites W2763878102 @default.
• W2894621070 cites W2768226911 @default.
• W2894621070 cites W2783501849 @default.
• W2894621070 cites W2800594509 @default.
• W2894621070 cites W2806820493 @default.
• W2894621070 cites W2807198924 @default.
• W2894621070 cites W4231033628 @default.
• W2894621070 cites W4245053291 @default.
• W2894621070 doi "https://doi.org/10.1038/s41598-018-33244-x" @default.
• W2894621070 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6181960" @default.

• next