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- W2894699814 abstract "Abstract Cytoplasmic calcium transients relay cellular signals on timescales from milliseconds to hours, and the dynamic nature of calcium signals has slowed functional genomic screening for cellular calcium effectors. Here, we present a new strategy to identify calcium handling genes via a pooled knockdown employing the calcium-sensitive photo-switchable fluorescent protein, CaMPARI. This assay, cal-Seq, enabled identification of regulators for both cellular desensitization and histamine induced calcium signaling including GPR99, a leukotriene binding receptor, with implications in asthma treatment." @default.
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- W2894699814 date "2018-10-04" @default.
- W2894699814 modified "2023-09-23" @default.
- W2894699814 title "Pooled functional genomic screens for intracellular calcium effectors" @default.
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- W2894699814 doi "https://doi.org/10.1101/434910" @default.
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