Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894747434> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2894747434 abstract "Introduction: Cardiosphere-derived cells (CDCs) possess regenerative, immunomodulatory and cardioprotective characteristics when delivered to the heart post-myocardial infarction (MI). CDCs derived from different donors possess variable regenerative potency in a MI model. We previously demonstrated that human CDC-derived extracellular vesicles (CDC-EVs) recapitulate the effects of CDCs in acute and chronic in vivo MI models. We are testing the hypothesis that a distinct cargo profile defines the functional efficacy between CDC-EVs and mesenchymal stem cell-derived EVs (MSC-EVs). Also, we assessed the immunomodulatory role of CDC-EVs derived from potent and non-potent CDCs in comparison with MSC-EVs. Methods: CDCs or MSCs were cultured in serum-free medium for 15 days and the conditioned media was concentrated by ultrafiltration to isolate EVs. The miRNeasy Serum/Plasma kit was used to extract total RNA from EVs followed by small RNA sequencing (NextSeq 500, Illumina) (CDC-EVs, n =12; MSC-EVs, n =4). We performed an in vitro and in vivo macrophage assay to assess the immunomodulatory role of EVs. Results: RNA sequencing analysis of CDC-EVs and MSC-EVs revealed a greater overall abundance of Y-RNA fragments and miRNA in CDC-EVs. Examining the specific Y-RNA classes, we found that MSC-EVs contained an expression profile with lower Y4 (p<0.05) but higher Y5 (p<0.05) Y-RNA fragments. Four miRNAs (miR-146a, miR-151, miR-409, miR-423) were highly enriched in CDC-EVs while miR-10a and miR-4792 were more abundant in MSC-EVs. EVs from potent CDCs showed a stronger upregulation of anti-inflammatory genes in activated macrophages ( in vitro ) and prevented accumulation of macrophages ( in vivo ) when compared with EVs obtained from non-potent CDCs or MSCs. Conclusions: We demonstrated that CDC-EVs contain a unique RNA cargo set that can differentiate CDC-EVs from MSC-EVs. The higher presence of Y-RNA in CDC-EVs as compared to MSC-EVs could be responsible for the ability of CDCs to regulate stem cell activation and tissue regeneration. The highly enriched panel of miRNAs observed in CDC-EVs and the ability to reduce the inflammatory reaction by macrophages, in contrast to MSC-EVs, suggest that they may support some of the functional benefits observed post-MI." @default.
- W2894747434 created "2018-10-12" @default.
- W2894747434 creator A5014169253 @default.
- W2894747434 creator A5024901700 @default.
- W2894747434 creator A5037661247 @default.
- W2894747434 creator A5060760961 @default.
- W2894747434 creator A5069866872 @default.
- W2894747434 creator A5089229037 @default.
- W2894747434 date "2017-11-14" @default.
- W2894747434 modified "2023-10-12" @default.
- W2894747434 title "Abstract 21281: Cardiosphere-Derived Cell and Mesenchymal Stem Cell Extracellular Vesicles Contain Distinct RNA Cargo" @default.
- W2894747434 doi "https://doi.org/10.1161/circ.136.suppl_1.21281" @default.
- W2894747434 hasPublicationYear "2017" @default.
- W2894747434 type Work @default.
- W2894747434 sameAs 2894747434 @default.
- W2894747434 citedByCount "1" @default.
- W2894747434 countsByYear W28947474342020 @default.
- W2894747434 crossrefType "journal-article" @default.
- W2894747434 hasAuthorship W2894747434A5014169253 @default.
- W2894747434 hasAuthorship W2894747434A5024901700 @default.
- W2894747434 hasAuthorship W2894747434A5037661247 @default.
- W2894747434 hasAuthorship W2894747434A5060760961 @default.
- W2894747434 hasAuthorship W2894747434A5069866872 @default.
- W2894747434 hasAuthorship W2894747434A5089229037 @default.
- W2894747434 hasConcept C104317684 @default.
- W2894747434 hasConcept C145059251 @default.
- W2894747434 hasConcept C1491633281 @default.
- W2894747434 hasConcept C153911025 @default.
- W2894747434 hasConcept C198826908 @default.
- W2894747434 hasConcept C202751555 @default.
- W2894747434 hasConcept C207001950 @default.
- W2894747434 hasConcept C28328180 @default.
- W2894747434 hasConcept C2992929900 @default.
- W2894747434 hasConcept C54355233 @default.
- W2894747434 hasConcept C55493867 @default.
- W2894747434 hasConcept C67705224 @default.
- W2894747434 hasConcept C71924100 @default.
- W2894747434 hasConcept C86803240 @default.
- W2894747434 hasConcept C95444343 @default.
- W2894747434 hasConceptScore W2894747434C104317684 @default.
- W2894747434 hasConceptScore W2894747434C145059251 @default.
- W2894747434 hasConceptScore W2894747434C1491633281 @default.
- W2894747434 hasConceptScore W2894747434C153911025 @default.
- W2894747434 hasConceptScore W2894747434C198826908 @default.
- W2894747434 hasConceptScore W2894747434C202751555 @default.
- W2894747434 hasConceptScore W2894747434C207001950 @default.
- W2894747434 hasConceptScore W2894747434C28328180 @default.
- W2894747434 hasConceptScore W2894747434C2992929900 @default.
- W2894747434 hasConceptScore W2894747434C54355233 @default.
- W2894747434 hasConceptScore W2894747434C55493867 @default.
- W2894747434 hasConceptScore W2894747434C67705224 @default.
- W2894747434 hasConceptScore W2894747434C71924100 @default.
- W2894747434 hasConceptScore W2894747434C86803240 @default.
- W2894747434 hasConceptScore W2894747434C95444343 @default.
- W2894747434 hasIssue "suppl_1" @default.
- W2894747434 hasLocation W28947474341 @default.
- W2894747434 hasOpenAccess W2894747434 @default.
- W2894747434 hasPrimaryLocation W28947474341 @default.
- W2894747434 hasRelatedWork W1756493412 @default.
- W2894747434 hasRelatedWork W2328203880 @default.
- W2894747434 hasRelatedWork W2351470921 @default.
- W2894747434 hasRelatedWork W2536437376 @default.
- W2894747434 hasRelatedWork W2587832528 @default.
- W2894747434 hasRelatedWork W2971352022 @default.
- W2894747434 hasRelatedWork W3004427646 @default.
- W2894747434 hasRelatedWork W3088353119 @default.
- W2894747434 hasRelatedWork W4321378868 @default.
- W2894747434 hasRelatedWork W4361215266 @default.
- W2894747434 hasVolume "136" @default.
- W2894747434 isParatext "false" @default.
- W2894747434 isRetracted "false" @default.
- W2894747434 magId "2894747434" @default.
- W2894747434 workType "article" @default.