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• W2894771333 endingPage "3016" @default.
• W2894771333 startingPage "3016" @default.
• W2894771333 abstract "Eukaryotic lysyl-tRNA synthetases (LysRS) have an N-terminal appended tRNA-interaction domain (RID) that is absent in their prokaryotic counterparts. This domain is intrinsically disordered and lacks stable structures. The disorder-to-order transition is induced by tRNA binding and has implications on folding and subsequent assembly into multi-tRNA synthetase complexes. Here, we expressed and purified RID from human LysRS (hRID) in Escherichia coli and performed a detailed mutagenesis of the appended domain. hRID was co-purified with nucleic acids during Ni-affinity purification, and cumulative mutations on critical amino acid residues abolished RNA binding. Furthermore, we identified a structural ensemble between disordered and helical structures in non-RNA-binding mutants and an equilibrium shift for wild-type into the helical conformation upon RNA binding. Since mutations that disrupted RNA binding led to an increase in non-functional soluble aggregates, a stabilized RNA-mediated structural transition of the N-terminal appended domain may have implications on the functional organization of human LysRS and multi-tRNA synthetase complexes in vivo." @default.
• W2894771333 created "2018-10-12" @default.
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• W2894771333 date "2018-10-03" @default.
• W2894771333 modified "2023-09-27" @default.
• W2894771333 title "Nucleic Acid-Dependent Structural Transition of the Intrinsically Disordered N-Terminal Appended Domain of Human Lysyl-tRNA Synthetase" @default.
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• W2894771333 doi "https://doi.org/10.3390/ijms19103016" @default.
• W2894771333 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6213541" @default.
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• W2894771333 hasPublicationYear "2018" @default.
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