Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894792117> ?p ?o ?g. }
- W2894792117 endingPage "449" @default.
- W2894792117 startingPage "441" @default.
- W2894792117 abstract "Proteins harboring peroxisome-targeting signal type-2 (PTS2) are recognized in the cytosol by mobile PTS2 receptor Pex7p and associate with a longer isoform Pex5pL of the PTS1 receptor. Trimeric PTS2 protein-Pex7p-Pex5pL complexes are translocated to peroxisomes in mammalian cells. However, it remains unclear whether Pex5pL and Pex7p are sufficient cytosolic components in transporting of PTS2 proteins to peroxisomes. Here, we construct a semi-intact cell import system to define the cytosolic components required for the peroxisomal PTS2 protein import and show that the PTS2 pre-import complexes comprising Pex7p, Pex5p, and Hsc70 isolated from the cytosol of pex14 Chinese hamster ovary cell mutant ZP161 is import-competent. PTS2 reporter proteins are transported to peroxisomes by recombinant Pex7p and Pex5pL in semi-intact cells devoid of the cytosol. Furthermore, PTS2 proteins are translocated to peroxisomes in the presence of a non-hydrolyzable ATP analogue, adenylyl imidodiphosphate, and N-ethylmaleimide, suggesting that ATP-dependent chaperones including Hsc70 are dispensable for PTS2 protein import. Taken together, we suggest that Pex7p and Pex5pL are the minimal cytosolic factors in the transport of PTS2 proteins to peroxisomes." @default.
- W2894792117 created "2018-10-12" @default.
- W2894792117 creator A5016823828 @default.
- W2894792117 creator A5035276490 @default.
- W2894792117 creator A5053489078 @default.
- W2894792117 date "2019-03-01" @default.
- W2894792117 modified "2023-10-13" @default.
- W2894792117 title "The cytosolic peroxisome-targeting signal (PTS)-receptors, Pex7p and Pex5pL, are sufficient to transport PTS2 proteins to peroxisomes" @default.
- W2894792117 cites W1589268957 @default.
- W2894792117 cites W1969836693 @default.
- W2894792117 cites W1969909007 @default.
- W2894792117 cites W1973185815 @default.
- W2894792117 cites W1976257663 @default.
- W2894792117 cites W1982255172 @default.
- W2894792117 cites W1987049546 @default.
- W2894792117 cites W1998843625 @default.
- W2894792117 cites W2006120283 @default.
- W2894792117 cites W2006605310 @default.
- W2894792117 cites W2008344765 @default.
- W2894792117 cites W2010813706 @default.
- W2894792117 cites W2014492708 @default.
- W2894792117 cites W2016619307 @default.
- W2894792117 cites W2025104020 @default.
- W2894792117 cites W2033150452 @default.
- W2894792117 cites W2037267510 @default.
- W2894792117 cites W2038731887 @default.
- W2894792117 cites W2043414371 @default.
- W2894792117 cites W2045328094 @default.
- W2894792117 cites W2047561285 @default.
- W2894792117 cites W2054725287 @default.
- W2894792117 cites W2060624652 @default.
- W2894792117 cites W2061772847 @default.
- W2894792117 cites W2073972553 @default.
- W2894792117 cites W2076526497 @default.
- W2894792117 cites W2081869877 @default.
- W2894792117 cites W2089720495 @default.
- W2894792117 cites W2090022626 @default.
- W2894792117 cites W2100174362 @default.
- W2894792117 cites W2104042107 @default.
- W2894792117 cites W2105818941 @default.
- W2894792117 cites W2107528894 @default.
- W2894792117 cites W2115992493 @default.
- W2894792117 cites W2121077965 @default.
- W2894792117 cites W2130310848 @default.
- W2894792117 cites W2130350654 @default.
- W2894792117 cites W2132762612 @default.
- W2894792117 cites W2140309519 @default.
- W2894792117 cites W2141199733 @default.
- W2894792117 cites W2145637864 @default.
- W2894792117 cites W2147615420 @default.
- W2894792117 cites W2149312404 @default.
- W2894792117 cites W2155571141 @default.
- W2894792117 cites W2170566884 @default.
- W2894792117 cites W2171631681 @default.
- W2894792117 cites W2413107236 @default.
- W2894792117 cites W4235648474 @default.
- W2894792117 cites W4243791482 @default.
- W2894792117 doi "https://doi.org/10.1016/j.bbamcr.2018.10.006" @default.
- W2894792117 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30296498" @default.
- W2894792117 hasPublicationYear "2019" @default.
- W2894792117 type Work @default.
- W2894792117 sameAs 2894792117 @default.
- W2894792117 citedByCount "4" @default.
- W2894792117 countsByYear W28947921172020 @default.
- W2894792117 countsByYear W28947921172022 @default.
- W2894792117 countsByYear W28947921172023 @default.
- W2894792117 crossrefType "journal-article" @default.
- W2894792117 hasAuthorship W2894792117A5016823828 @default.
- W2894792117 hasAuthorship W2894792117A5035276490 @default.
- W2894792117 hasAuthorship W2894792117A5053489078 @default.
- W2894792117 hasConcept C12296475 @default.
- W2894792117 hasConcept C127078168 @default.
- W2894792117 hasConcept C144647389 @default.
- W2894792117 hasConcept C170493617 @default.
- W2894792117 hasConcept C175656101 @default.
- W2894792117 hasConcept C181199279 @default.
- W2894792117 hasConcept C205668238 @default.
- W2894792117 hasConcept C41625074 @default.
- W2894792117 hasConcept C55493867 @default.
- W2894792117 hasConcept C559089031 @default.
- W2894792117 hasConcept C86803240 @default.
- W2894792117 hasConcept C95444343 @default.
- W2894792117 hasConcept C98539663 @default.
- W2894792117 hasConceptScore W2894792117C12296475 @default.
- W2894792117 hasConceptScore W2894792117C127078168 @default.
- W2894792117 hasConceptScore W2894792117C144647389 @default.
- W2894792117 hasConceptScore W2894792117C170493617 @default.
- W2894792117 hasConceptScore W2894792117C175656101 @default.
- W2894792117 hasConceptScore W2894792117C181199279 @default.
- W2894792117 hasConceptScore W2894792117C205668238 @default.
- W2894792117 hasConceptScore W2894792117C41625074 @default.
- W2894792117 hasConceptScore W2894792117C55493867 @default.
- W2894792117 hasConceptScore W2894792117C559089031 @default.
- W2894792117 hasConceptScore W2894792117C86803240 @default.
- W2894792117 hasConceptScore W2894792117C95444343 @default.
- W2894792117 hasConceptScore W2894792117C98539663 @default.
- W2894792117 hasFunder F4320309785 @default.
- W2894792117 hasFunder F4320309807 @default.