Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894829776> ?p ?o ?g. }
- W2894829776 abstract "Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efficacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested flavoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable differences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specific ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications." @default.
- W2894829776 created "2018-10-12" @default.
- W2894829776 creator A5002529655 @default.
- W2894829776 creator A5013903576 @default.
- W2894829776 creator A5023198743 @default.
- W2894829776 creator A5036184011 @default.
- W2894829776 creator A5036391421 @default.
- W2894829776 creator A5037481887 @default.
- W2894829776 creator A5038665783 @default.
- W2894829776 creator A5043556804 @default.
- W2894829776 creator A5075523221 @default.
- W2894829776 creator A5078064862 @default.
- W2894829776 creator A5086587711 @default.
- W2894829776 creator A5091850237 @default.
- W2894829776 date "2018-10-09" @default.
- W2894829776 modified "2023-10-18" @default.
- W2894829776 title "An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria" @default.
- W2894829776 cites W128291326 @default.
- W2894829776 cites W1439626848 @default.
- W2894829776 cites W1498884303 @default.
- W2894829776 cites W1501355593 @default.
- W2894829776 cites W1531576924 @default.
- W2894829776 cites W1820385459 @default.
- W2894829776 cites W1853926988 @default.
- W2894829776 cites W1857647405 @default.
- W2894829776 cites W1862187459 @default.
- W2894829776 cites W1902000402 @default.
- W2894829776 cites W1964376228 @default.
- W2894829776 cites W1964772492 @default.
- W2894829776 cites W1966160892 @default.
- W2894829776 cites W1972936647 @default.
- W2894829776 cites W1978824717 @default.
- W2894829776 cites W1981206315 @default.
- W2894829776 cites W1987531894 @default.
- W2894829776 cites W1988824102 @default.
- W2894829776 cites W1989732798 @default.
- W2894829776 cites W1990540842 @default.
- W2894829776 cites W1991383594 @default.
- W2894829776 cites W1991901212 @default.
- W2894829776 cites W1992451364 @default.
- W2894829776 cites W2002144817 @default.
- W2894829776 cites W2008900126 @default.
- W2894829776 cites W2009734791 @default.
- W2894829776 cites W2010983045 @default.
- W2894829776 cites W2011087372 @default.
- W2894829776 cites W2011995125 @default.
- W2894829776 cites W2013404730 @default.
- W2894829776 cites W2013878893 @default.
- W2894829776 cites W2018964965 @default.
- W2894829776 cites W2020065174 @default.
- W2894829776 cites W2026596261 @default.
- W2894829776 cites W2027282106 @default.
- W2894829776 cites W2027516320 @default.
- W2894829776 cites W2027908623 @default.
- W2894829776 cites W2030566353 @default.
- W2894829776 cites W2031680497 @default.
- W2894829776 cites W2033950520 @default.
- W2894829776 cites W2042630054 @default.
- W2894829776 cites W2043281094 @default.
- W2894829776 cites W2044941847 @default.
- W2894829776 cites W2045631041 @default.
- W2894829776 cites W2047881665 @default.
- W2894829776 cites W2048599236 @default.
- W2894829776 cites W2049619102 @default.
- W2894829776 cites W2050106587 @default.
- W2894829776 cites W2052107807 @default.
- W2894829776 cites W2052751103 @default.
- W2894829776 cites W2053068822 @default.
- W2894829776 cites W2058203897 @default.
- W2894829776 cites W2058964632 @default.
- W2894829776 cites W2060081618 @default.
- W2894829776 cites W2065098039 @default.
- W2894829776 cites W2067492027 @default.
- W2894829776 cites W2068410503 @default.
- W2894829776 cites W2069795071 @default.
- W2894829776 cites W2072665697 @default.
- W2894829776 cites W2075795099 @default.
- W2894829776 cites W2076813288 @default.
- W2894829776 cites W2084471538 @default.
- W2894829776 cites W2085019719 @default.
- W2894829776 cites W2087073963 @default.
- W2894829776 cites W2093088311 @default.
- W2894829776 cites W2103824933 @default.
- W2894829776 cites W2109037644 @default.
- W2894829776 cites W2118590734 @default.
- W2894829776 cites W2130665679 @default.
- W2894829776 cites W2132012821 @default.
- W2894829776 cites W2132417094 @default.
- W2894829776 cites W2134701031 @default.
- W2894829776 cites W2136956732 @default.
- W2894829776 cites W2141402655 @default.
- W2894829776 cites W2147119806 @default.
- W2894829776 cites W2150019542 @default.
- W2894829776 cites W2151734986 @default.
- W2894829776 cites W2154405539 @default.
- W2894829776 cites W2154799707 @default.
- W2894829776 cites W2154802301 @default.
- W2894829776 cites W2165723638 @default.
- W2894829776 cites W2167233919 @default.
- W2894829776 cites W2208561385 @default.