Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894835548> ?p ?o ?g. }
- W2894835548 endingPage "372" @default.
- W2894835548 startingPage "372" @default.
- W2894835548 abstract "Haloferax mediterranei produces C50 carotenoids that have strong antioxidant properties. The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditions to maximize C50 carotenoids production by haloarchaea. The effects of temperature (15–50 °C), pH (4−10), and salinity (5–28% NaCl (w/v)) on the growth and carotenoid content of H. mediterranei were analyzed using the RSM approach. Growth was determined by measuring the turbidity at 600 nm. To determine the carotenoid content, harvested cells were lysed by freeze/thawing, then re-suspended in acetone and the total carotenoid content determined by measuring the absorbance at 494 nm. The analysis of carotenoids was performed by an HPLC system coupled with mass spectrometry. The results indicated the theoretical optimal conditions of 36.51 or 36.81 °C, pH of 8.20 or 8.96, and 15.01% or 12.03% (w/v) salinity for the growth of haloarchaea (OD600 = 12.5 ± 0.64) and production of total carotenoids (3.34 ± 0.29 mg/L), respectively. These conditions were validated experimentally for growth (OD600 = 13.72 ± 0.98) and carotenoid production (3.74 ± 0.20 mg/L). The carotenoid profile showed four isomers of bacterioruberin (89.13%). Our findings suggest that the RSM approach is highly useful for determining optimal conditions for large-scale production of bacterioruberin by haloarchaea." @default.
- W2894835548 created "2018-10-12" @default.
- W2894835548 creator A5015067739 @default.
- W2894835548 creator A5020060705 @default.
- W2894835548 creator A5025812919 @default.
- W2894835548 creator A5030585003 @default.
- W2894835548 creator A5041328917 @default.
- W2894835548 creator A5066291071 @default.
- W2894835548 creator A5082836040 @default.
- W2894835548 creator A5084147656 @default.
- W2894835548 creator A5091551158 @default.
- W2894835548 date "2018-10-09" @default.
- W2894835548 modified "2023-10-18" @default.
- W2894835548 title "Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology" @default.
- W2894835548 cites W1709969568 @default.
- W2894835548 cites W1948725849 @default.
- W2894835548 cites W1965229960 @default.
- W2894835548 cites W1995631091 @default.
- W2894835548 cites W2002498973 @default.
- W2894835548 cites W2007207750 @default.
- W2894835548 cites W2039192484 @default.
- W2894835548 cites W2053479365 @default.
- W2894835548 cites W2067458052 @default.
- W2894835548 cites W2073186739 @default.
- W2894835548 cites W2080848279 @default.
- W2894835548 cites W2084340897 @default.
- W2894835548 cites W2091567961 @default.
- W2894835548 cites W2106144475 @default.
- W2894835548 cites W2107034381 @default.
- W2894835548 cites W2110517513 @default.
- W2894835548 cites W2116566710 @default.
- W2894835548 cites W2127406851 @default.
- W2894835548 cites W2153389493 @default.
- W2894835548 cites W2153394912 @default.
- W2894835548 cites W2161741112 @default.
- W2894835548 cites W2169894432 @default.
- W2894835548 cites W2237477864 @default.
- W2894835548 cites W2292286035 @default.
- W2894835548 cites W2320409770 @default.
- W2894835548 cites W2743631750 @default.
- W2894835548 cites W2782884141 @default.
- W2894835548 cites W2807342767 @default.
- W2894835548 cites W4229547445 @default.
- W2894835548 doi "https://doi.org/10.3390/md16100372" @default.
- W2894835548 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6213265" @default.
- W2894835548 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30304770" @default.
- W2894835548 hasPublicationYear "2018" @default.
- W2894835548 type Work @default.
- W2894835548 sameAs 2894835548 @default.
- W2894835548 citedByCount "30" @default.
- W2894835548 countsByYear W28948355482018 @default.
- W2894835548 countsByYear W28948355482019 @default.
- W2894835548 countsByYear W28948355482020 @default.
- W2894835548 countsByYear W28948355482021 @default.
- W2894835548 countsByYear W28948355482022 @default.
- W2894835548 countsByYear W28948355482023 @default.
- W2894835548 crossrefType "journal-article" @default.
- W2894835548 hasAuthorship W2894835548A5015067739 @default.
- W2894835548 hasAuthorship W2894835548A5020060705 @default.
- W2894835548 hasAuthorship W2894835548A5025812919 @default.
- W2894835548 hasAuthorship W2894835548A5030585003 @default.
- W2894835548 hasAuthorship W2894835548A5041328917 @default.
- W2894835548 hasAuthorship W2894835548A5066291071 @default.
- W2894835548 hasAuthorship W2894835548A5082836040 @default.
- W2894835548 hasAuthorship W2894835548A5084147656 @default.
- W2894835548 hasAuthorship W2894835548A5091551158 @default.
- W2894835548 hasBestOaLocation W28948355481 @default.
- W2894835548 hasConcept C150077022 @default.
- W2894835548 hasConcept C185592680 @default.
- W2894835548 hasConcept C2779926853 @default.
- W2894835548 hasConcept C28781525 @default.
- W2894835548 hasConcept C31903555 @default.
- W2894835548 hasConcept C43617362 @default.
- W2894835548 hasConcept C523546767 @default.
- W2894835548 hasConcept C54355233 @default.
- W2894835548 hasConcept C80642116 @default.
- W2894835548 hasConcept C86803240 @default.
- W2894835548 hasConceptScore W2894835548C150077022 @default.
- W2894835548 hasConceptScore W2894835548C185592680 @default.
- W2894835548 hasConceptScore W2894835548C2779926853 @default.
- W2894835548 hasConceptScore W2894835548C28781525 @default.
- W2894835548 hasConceptScore W2894835548C31903555 @default.
- W2894835548 hasConceptScore W2894835548C43617362 @default.
- W2894835548 hasConceptScore W2894835548C523546767 @default.
- W2894835548 hasConceptScore W2894835548C54355233 @default.
- W2894835548 hasConceptScore W2894835548C80642116 @default.
- W2894835548 hasConceptScore W2894835548C86803240 @default.
- W2894835548 hasFunder F4320326262 @default.
- W2894835548 hasIssue "10" @default.
- W2894835548 hasLocation W28948355481 @default.
- W2894835548 hasLocation W28948355482 @default.
- W2894835548 hasLocation W28948355483 @default.
- W2894835548 hasLocation W28948355484 @default.
- W2894835548 hasLocation W28948355485 @default.
- W2894835548 hasLocation W28948355486 @default.
- W2894835548 hasLocation W28948355487 @default.
- W2894835548 hasOpenAccess W2894835548 @default.
- W2894835548 hasPrimaryLocation W28948355481 @default.