FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894888949> ?p ?o ?g. }

• W2894888949 endingPage "10485" @default.
• W2894888949 startingPage "10473" @default.
• W2894888949 abstract "Multivalent presentation of ligands on nanoparticles (NPs) is considered a general strategy for enhancing receptor binding and activation through amplification of ligand–receptor interactions within the footprint of the individual NPs. The spatial clustering of ligand-functionalized NPs represents an additional, less well understood mechanism for increasing local ligand–receptor interactions, especially for receptors that form higher-order assemblies, such as the epidermal growth factor (EGF) receptor (EGFR). To shed light on the interplay between ligand density (i.e., multivalency) and NP clustering in signal amplification, we apply EGF-functionalized 72 ± 1 nm gold nanoparticles (NP-EGF) with known ligand loading (10–200 EGF/NP) as quantifiable and experimentally tractable units of EGFR activation and characterize the NP-mediated amplification of EGFR phosphorylation as a function of both EGF surface density and NP-EGF clustering for two cancer cell lines (HeLa and MDA-MB-468). The measurements confirm a strong multivalent amplification of EGFR phosphorylation through NP-EGF on the cellular level that results in EGF-loading-dependent maximum EGFR phosphorylation levels. A microscopic analysis of NP-EGF-induced EGFR phosphorylation reveals a heterogeneous spatial distribution of EGFR activation across the cell surface. Clustering of multivalent NP-EGF on sub-diffraction-limited length scales is found to result in a local enhancement of EGFR phosphorylation in signaling “hot spots” from where the signal can spread laterally in an EGF-independent fashion. Increasing EGF loadings of the NP enhances NP-EGF clustering and intensifies EGFR phosphorylation. These observations suggest that NP-EGF clustering and the associated local enhancement of ligand–receptor interactions are intrinsic components of the multivalent amplification of phosphorylation for the heterogeneously distributed EGFR through NP-EGF." @default.
• W2894888949 created "2018-10-12" @default.
• W2894888949 creator A5052563154 @default.
• W2894888949 creator A5067330655 @default.
• W2894888949 date "2018-10-05" @default.
• W2894888949 modified "2023-10-05" @default.
• W2894888949 title "Ligand Density and Nanoparticle Clustering Cooperate in the Multivalent Amplification of Epidermal Growth Factor Receptor Activation" @default.
• W2894888949 cites W1556801875 @default.
• W2894888949 cites W1972326799 @default.
• W2894888949 cites W1973549619 @default.
• W2894888949 cites W1973682361 @default.
• W2894888949 cites W1978870288 @default.
• W2894888949 cites W1979884986 @default.
• W2894888949 cites W1984048831 @default.
• W2894888949 cites W1986526693 @default.
• W2894888949 cites W1995592923 @default.
• W2894888949 cites W1995929412 @default.
• W2894888949 cites W1996139192 @default.
• W2894888949 cites W2001395998 @default.
• W2894888949 cites W2002429753 @default.
• W2894888949 cites W2004950702 @default.
• W2894888949 cites W2013301709 @default.
• W2894888949 cites W2016118559 @default.
• W2894888949 cites W2020343586 @default.
• W2894888949 cites W2022050193 @default.
• W2894888949 cites W2026772781 @default.
• W2894888949 cites W2028415754 @default.
• W2894888949 cites W2029733869 @default.
• W2894888949 cites W2033342041 @default.
• W2894888949 cites W2035697316 @default.
• W2894888949 cites W2041060455 @default.
• W2894888949 cites W2048134161 @default.
• W2894888949 cites W2050922236 @default.
• W2894888949 cites W2052731133 @default.
• W2894888949 cites W2054165134 @default.
• W2894888949 cites W2055079936 @default.
• W2894888949 cites W2058668004 @default.
• W2894888949 cites W2062560208 @default.
• W2894888949 cites W2062706311 @default.
• W2894888949 cites W2062855968 @default.
• W2894888949 cites W2063170675 @default.
• W2894888949 cites W2068389027 @default.
• W2894888949 cites W2070782703 @default.
• W2894888949 cites W2071522285 @default.
• W2894888949 cites W2074929394 @default.
• W2894888949 cites W2077663689 @default.
• W2894888949 cites W2078314122 @default.
• W2894888949 cites W2078998802 @default.
• W2894888949 cites W2079116168 @default.
• W2894888949 cites W2080374932 @default.
• W2894888949 cites W2083219195 @default.
• W2894888949 cites W2087950086 @default.
• W2894888949 cites W2088575565 @default.
• W2894888949 cites W2096406493 @default.
• W2894888949 cites W2097097666 @default.
• W2894888949 cites W2097649748 @default.
• W2894888949 cites W2098421622 @default.
• W2894888949 cites W2098684433 @default.
• W2894888949 cites W2108524724 @default.
• W2894888949 cites W2109538480 @default.
• W2894888949 cites W2110039009 @default.
• W2894888949 cites W2111309386 @default.
• W2894888949 cites W2113125756 @default.
• W2894888949 cites W2113681314 @default.
• W2894888949 cites W2121830147 @default.
• W2894888949 cites W2125782175 @default.
• W2894888949 cites W2126044151 @default.
• W2894888949 cites W2126818937 @default.
• W2894888949 cites W2128645999 @default.
• W2894888949 cites W2135858378 @default.
• W2894888949 cites W2143668880 @default.
• W2894888949 cites W2150737519 @default.
• W2894888949 cites W2153231341 @default.
• W2894888949 cites W2160234058 @default.
• W2894888949 cites W2163322383 @default.
• W2894888949 cites W2164565944 @default.
• W2894888949 cites W2167279371 @default.
• W2894888949 cites W2171083652 @default.
• W2894888949 cites W2179854480 @default.
• W2894888949 cites W2219921058 @default.
• W2894888949 cites W2250126128 @default.
• W2894888949 cites W2269746046 @default.
• W2894888949 cites W2273495465 @default.
• W2894888949 cites W2293347166 @default.
• W2894888949 cites W2298212394 @default.
• W2894888949 cites W2319906704 @default.
• W2894888949 cites W2321443019 @default.
• W2894888949 cites W2325657998 @default.
• W2894888949 cites W2327950553 @default.
• W2894888949 cites W2328674117 @default.
• W2894888949 cites W2330658771 @default.
• W2894888949 cites W2543947893 @default.
• W2894888949 cites W2552997266 @default.
• W2894888949 cites W2600590737 @default.
• W2894888949 cites W2771253477 @default.
• W2894888949 cites W4211075026 @default.
• W2894888949 cites W4256503849 @default.
• W2894888949 doi "https://doi.org/10.1021/acsnano.8b06141" @default.

• next