Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894923276> ?p ?o ?g. }
- W2894923276 abstract "Abstract Transposon Directed Insertion Sequencing (TraDIS) is a high-throughput method for mapping insertion sites in large libraries of transposon mutants. The Himar1 ( mariner ) transposon is ideal for generating near-saturating mutant libraries, especially in AT-rich chromosomes, as the requirement for integration is a TA dinucleotide. In this study, we generated two novel mariner vectors, pTsodCPC9 and pTlacPC9 (differing only in the promoter driving expression of the transposase gene), in order to facilitate TraDIS identification of conditionally essential genes in Actinobacillus pleuropneumoniae and other bacteria. Using the pTlacPC9 vector, we have generated, for the first time, saturating mariner mutant libraries in both A. pleuropneumoniae and Pasteurella multocida that showed a near random distribution of insertions around the respective chromosomes. A preliminary screen of 5000 mutants each identified 8 and 15 genes, respectively, that are required for growth under anaerobic conditions." @default.
- W2894923276 created "2018-10-12" @default.
- W2894923276 creator A5026819067 @default.
- W2894923276 creator A5030671607 @default.
- W2894923276 creator A5034559903 @default.
- W2894923276 creator A5037861078 @default.
- W2894923276 creator A5046262151 @default.
- W2894923276 creator A5047037920 @default.
- W2894923276 creator A5054000844 @default.
- W2894923276 creator A5055901471 @default.
- W2894923276 creator A5068855554 @default.
- W2894923276 creator A5071675129 @default.
- W2894923276 creator A5079165359 @default.
- W2894923276 creator A5085081570 @default.
- W2894923276 creator A5085432762 @default.
- W2894923276 creator A5087922615 @default.
- W2894923276 date "2018-10-03" @default.
- W2894923276 modified "2023-09-23" @default.
- W2894923276 title "Rationally designed mariner vectors to allow functional genomic analysis of Actinobacillus pleuropneumoniae and other bacteria by transposon-directed insertion-site sequencing (TraDIS)" @default.
- W2894923276 cites W1568951356 @default.
- W2894923276 cites W1741795223 @default.
- W2894923276 cites W1970330058 @default.
- W2894923276 cites W1971323277 @default.
- W2894923276 cites W1977202686 @default.
- W2894923276 cites W1980214739 @default.
- W2894923276 cites W1980780067 @default.
- W2894923276 cites W1986618222 @default.
- W2894923276 cites W1994050060 @default.
- W2894923276 cites W2005718017 @default.
- W2894923276 cites W2007231114 @default.
- W2894923276 cites W2013902051 @default.
- W2894923276 cites W2014943744 @default.
- W2894923276 cites W2015783955 @default.
- W2894923276 cites W2018452208 @default.
- W2894923276 cites W2021735313 @default.
- W2894923276 cites W2029664323 @default.
- W2894923276 cites W2030924818 @default.
- W2894923276 cites W2045788063 @default.
- W2894923276 cites W2094819697 @default.
- W2894923276 cites W2096093282 @default.
- W2894923276 cites W2100009016 @default.
- W2894923276 cites W2100513000 @default.
- W2894923276 cites W2103911077 @default.
- W2894923276 cites W2105546078 @default.
- W2894923276 cites W2115523105 @default.
- W2894923276 cites W2116443594 @default.
- W2894923276 cites W2120852847 @default.
- W2894923276 cites W2120902911 @default.
- W2894923276 cites W2129879961 @default.
- W2894923276 cites W2136961240 @default.
- W2894923276 cites W2137369730 @default.
- W2894923276 cites W2139301092 @default.
- W2894923276 cites W2151451423 @default.
- W2894923276 cites W2152412632 @default.
- W2894923276 cites W2155790181 @default.
- W2894923276 cites W2158985540 @default.
- W2894923276 cites W2161733098 @default.
- W2894923276 cites W2163813592 @default.
- W2894923276 cites W2164466125 @default.
- W2894923276 cites W2169916860 @default.
- W2894923276 cites W2767189590 @default.
- W2894923276 cites W2784788330 @default.
- W2894923276 cites W3211973906 @default.
- W2894923276 cites W4250732031 @default.
- W2894923276 doi "https://doi.org/10.1101/433086" @default.
- W2894923276 hasPublicationYear "2018" @default.
- W2894923276 type Work @default.
- W2894923276 sameAs 2894923276 @default.
- W2894923276 citedByCount "2" @default.
- W2894923276 countsByYear W28949232762021 @default.
- W2894923276 countsByYear W28949232762022 @default.
- W2894923276 crossrefType "posted-content" @default.
- W2894923276 hasAuthorship W2894923276A5026819067 @default.
- W2894923276 hasAuthorship W2894923276A5030671607 @default.
- W2894923276 hasAuthorship W2894923276A5034559903 @default.
- W2894923276 hasAuthorship W2894923276A5037861078 @default.
- W2894923276 hasAuthorship W2894923276A5046262151 @default.
- W2894923276 hasAuthorship W2894923276A5047037920 @default.
- W2894923276 hasAuthorship W2894923276A5054000844 @default.
- W2894923276 hasAuthorship W2894923276A5055901471 @default.
- W2894923276 hasAuthorship W2894923276A5068855554 @default.
- W2894923276 hasAuthorship W2894923276A5071675129 @default.
- W2894923276 hasAuthorship W2894923276A5079165359 @default.
- W2894923276 hasAuthorship W2894923276A5085081570 @default.
- W2894923276 hasAuthorship W2894923276A5085432762 @default.
- W2894923276 hasAuthorship W2894923276A5087922615 @default.
- W2894923276 hasBestOaLocation W28949232761 @default.
- W2894923276 hasConcept C101985253 @default.
- W2894923276 hasConcept C104317684 @default.
- W2894923276 hasConcept C13575015 @default.
- W2894923276 hasConcept C143065580 @default.
- W2894923276 hasConcept C172510086 @default.
- W2894923276 hasConcept C2776796551 @default.
- W2894923276 hasConcept C28693550 @default.
- W2894923276 hasConcept C3017666073 @default.
- W2894923276 hasConcept C4918238 @default.
- W2894923276 hasConcept C51679486 @default.
- W2894923276 hasConcept C523546767 @default.
- W2894923276 hasConcept C54355233 @default.
- W2894923276 hasConcept C70721500 @default.