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- W2894935582 abstract "Human pluripotent stem cells (hPSCs) emerged as an important tool to investigate human development and disease. These studies often require genetically engineering hPSCs to stably express a transgene, which remains functional in various hPSC progeny. PiggyBac transposon is a highly effective and technically simple vector system with large cargo space available for permanent gene delivery. This unit describes the use of PiggyBac transposons to genetically engineer hPSCs to introduce conditionally expressed transgene or reporter to effectively monitor gene expression during differentiation. Both methods enable robust generation of stable hPSC lines within 1 month. © 2018 by John Wiley & Sons, Inc." @default.
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- W2894935582 date "2018-10-03" @default.
- W2894935582 modified "2023-10-17" @default.
- W2894935582 title "Genetic Engineering of Human Pluripotent Stem Cells Using PiggyBac Transposon System" @default.
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- W2894935582 doi "https://doi.org/10.1002/cpsc.63" @default.
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