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• W2894941214 abstract "Prior to harvest, maize kernels are invaded by a diverse population of fungal organisms that comprise the microbiome of the grain mass. Poor post-harvest practices and improper drying can lead to the growth of mycotoxigenic storage fungi and deterioration of grain quality. Hermetic storage bags are a low-cost technology for the preservation of grain during storage, which has seen significant adoption in many regions of Sub-Saharan Africa. This study explored the use of high-throughput DNA sequencing of the fungal Internal Transcribed Spacer 2 (ITS2) region for characterization of the fungal microbiome before and after three months of storage in hermetic and non-hermetic (woven) bags in the United States and Kenya. Analysis of 1,377,221 and 3,633,944 ITS2 sequences from the US and Kenya, respectively, resulted in 183 and 164 operational taxonomic units (OTUs). Taxonomic assignment of these OTUs revealed 63 and 34 fungal genera in the US and Kenya samples, respectively, many of which were not detected by traditional plating methods. The most abundant genus was Fusarium, which was identified in all samples. Storage fungi were detected in the grain mass prior to the storage experiments and increased in relative abundance within the woven bags. The results also indicate that storage location had no effect on the fungal microbiome of grain stored in the United States, while storage bag type led to significant changes in fungal composition. The fungal microbiome of the Kenya grain also underwent significant changes in composition during storage and fungal diversity increased during storage regardless of bag type. Our results indicated that extraction of DNA from ground kernels is sufficient for identifying the fungi associated with the maize. The results also indicated that bag type was the most important factor influencing changes in fungal microbiome during storage." @default.
• W2894941214 created "2018-10-12" @default.
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• W2894941214 date "2018-10-02" @default.
• W2894941214 modified "2023-10-17" @default.
• W2894941214 title "Changes in the Fungal Microbiome of Maize During Hermetic Storage in the United States and Kenya" @default.
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• W2894941214 doi "https://doi.org/10.3389/fmicb.2018.02336" @default.
• W2894941214 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6176044" @default.
• W2894941214 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30333810" @default.
• W2894941214 hasPublicationYear "2018" @default.
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